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Inducible expression of reconstructed human caspase-6 gene in Hela cells

, : Inducible expression of reconstructed human caspase-6 gene in Hela cells. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 19(3): 209-211

To observe the pro-apoptotic effect of RCasp-6 gene in Hela cells. RCasp-6 gene was amplified by PCR and cloned into the pIND vector. Hela cells were transfected with pIND-RCasp-6 and then inducced with ecdyson analogue. Expression of target gene was detected by immunocytochemical staining. Changes of the morphology and growth of Hela cells subjected to transfection of target gene were observed by HE staining and viable cell counting. RCasp-6 gene was cloned by PCR and its eukaryotic expression vector was successfully reconstructed. Expression of RCasp-6 gene in the transfected Hela cells leads to the morphological changes of the cells. Many of the transfected Hela cells shrunk and some cells were died. The expression of reconstructed human caspase-6 gene can efficiently accelerate death of Hela cells.


PMID: 15155071

Other references

Yuan, B.; Shin, J-Won.; Bernstein, E.R., 2016: Dynamics and fragmentation of van der Waals and hydrogen bonded cluster cations: (NH3)n and (NH3BH3)n ionized at 10.51 eV. A 118 nm laser is employed as a high energy, single photon (10.51 eV/photon) source for study of the dynamics and fragmentation of the ammonia borane (NH3BH3) cation and its cluster ions through time of flight mass spectrometry. The behavior of am...

Trevisol, D.J.; Moreira, L.B.; Kerkhoff, A.; Fuchs, S.C.; Fuchs, Fávio.D., 2011: Health-related quality of life and hypertension: a systematic review and meta-analysis of observational studies. Most, but not all cross-sectional surveys have identified lower quality of life among patients with hypertension. We summarized the estimates for this association in a systematic review and in a meta-analysis of cross-sectional studies. Studies th...

Mendoza-Alvarez, H.; Alvarez-Gonzalez, R., 2001: Regulation of p53 sequence-specific DNA-binding by covalent poly(ADP-ribosyl)ation. We have characterized the covalent poly(ADP-ribosyl)-ation of p53 using an in vitro reconstituted system. We used recombinant wild type p53, recombinant poly(ADP-ribose) polymerase-1 (PARP-1) (EC, and betaNAD+. Our results show that the...

Jones F.W.; Vozoff K., 1978: The calculation of magnetotelluric quantities for three-dimensional conductivity inhomogeneities. Three-dimensional numerical modeling is applied to magnetotelluric principal axis and tipper analysis. Calculations of the impedance, rotation angles, and tipper azimuths are made at the surface of a conducting half-space which contains perturbed...

Reig, Núria.; van der Goot, F.Gisou., 2006: About lipids and toxins. Many mono or multicellular organisms secrete soluble proteins, referred to as protein toxins, which alter the behavior of foreign, or target cells, possibly leading to their death. These toxins affect either the cell membrane by forming pores or m...

Takeuchi I.; Hamada Y.; Okamura K., 1989: Synthesis and crystal structure of naphtho 2 1 b 1 5 naphthyridine. A modified Skraup reaction was carried out on 2-aminobenzo [f] quinoline (1), in the presence of sulfo-mix, using ferrous sulfate and boric acid. This reaction gave the linear type product, naphtho-[2,1-b] [1, 5]naphthyridine (2), but not the angu...

Karen Acuff; Bill Broening; Kevin Crofton; Andrew Fix; Elizabeth Julien; Jay Nash; Ann Richard; Sarah Tozer; Chihae Yang, 2008: Conversion of Developmental Neurotoxicity (DNT) information into a structure-searchable relational database. Neurotoxicology and Teratology 30(3): 252-0

Venturi, F., 1972: Introduction to the study of the presumable mechanical functon of alar colourings and maculations of Diptera. Bollettino 31 79-107

Stünzi, H., 1965: Lung cancer from the viewpoint of comparative pathology. Schweizerische Medizinische Wochenschrift 95(51): 1744-1748

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