+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Infliximab treatment influences the serological expression of matrix metalloproteinase (MMP)-2 and -9 in Crohn's disease

, : Infliximab treatment influences the serological expression of matrix metalloproteinase (MMP)-2 and -9 in Crohn's disease. Inflammatory Bowel Diseases 13(6): 693-702

Matrix metalloproteinases (MMPs) are actively involved in the pathogenesis of Crohn's disease (CD). We assessed the effect of the anti-tumor necrosis factor-alpha (TNF-alpha) monoclonal antibody infliximab on the in vitro and in vivo expression of MMP-2 and MMP-9 in CD. Infliximab-treated fistulizing (n = 10) or active disease (n = 7) CD patients, from an in-house study, and fistulizing CD patients (n = 42) and active CD patients (n = 24) from 2 placebo controlled studies were evaluated for serum MMP levels and clinical response. Biopsies were evaluated immunohistochemically for the MMPs. Whole blood cultures stimulated with lipopolysaccharide (LPS)/infliximab were evaluated for MMP mRNA and protein levels. Serum MMP-2 levels in CD patients increased during follow-up, similarly in responders and nonresponders, by infliximab. Immunohistochemistry showed no clear MMP-2 change in biopsies. Serum MMP-9 levels, however, showed a consistent pattern of decrease in most CD patients, particularly in those responding, and MMP-9-positive polymorphonuclear leukocytes in biopsies also decreased by infliximab. LPS stimulation of whole blood increased the MMP-9 levels in plasma significantly in CD patients and controls, but infliximab had no effect on the secretion. Long-term LPS stimulation raised leukocyte MMP-9 mRNA levels 16-fold and infliximab inhibited this induction by 80%. Infliximab treatment increases MMP-2 and decreases MMP-9 in serum of patients with CD, the latter also in the intestine, which extends and confirms our previous ex vivo explants observations. However, these changes were not strictly associated with the response to treatment. The enhanced leukocyte MMP-9 expression in CD seems to be regulated by TNF-alpha.


PMID: 17243139

DOI: 10.1002/ibd.20100

Other references

Handsfield, H.H.; Lukehart, S.A.; Sell, S.; Norris, S.J.; Holmes, K.K., 1983: Demonstration of Treponema pallidum in a cutaneous gumma by indirect immunofluorescence. Treponema pallidum was demonstrated in a cutaneous, tertiary syphilitic lesion by indirect immunofluorescence microscopy, but not by darkfield microscopy illumination or silver stain. The numerous organisms observed by this method may help explain...

Nybonde, T.; Mortensson, W.; Robertson, B., 1983: Villonodular synovitis in the hip joint diagnosed by arthrography. Report of a case. Villonodular synovitis in the hip joint of a girl aged 13 years diagnosed by means of arthrography is reported. The value of arthrography in examination of obscure hip joint complaints is emphasized.

Schink, W., 1975: Local traumatic injury of the soft tissue and internal fixation. The influence of local traumatic injury of the soft tissue on the decision as to whether surgical therapy is indicated and on the method of internal fixation selected for a fresh, closed fracture is discussed. General descriptions of injuries to t...

Y.Huilong, 1987: The material source and the properties of ore-forming fluids of a stratabound antimony deposit in carbonate rocks at Xujiashan, Hubei. The Xujiashan sedimentary-reworked stratabound antimony deposit occurs in early Sinian marine carbonate rocks. Mineralization was controlled by the strata and structures. Stratoid, lenticular, and vein ore bodies are commonly observed. delta (supe...

Evangelista, P.; Evangelista, G., 2011: Dyspareunia associated with a pelvic digit. A pelvic digit is a rare congenital anomaly and often an incidental radiographic finding. Here we present the first reported case of a 57-year-old Caucasian female with a pelvic digit and a chief complaint of dyspareunia. Radiographic studies and...

Stallones, R.A., 1980: To advance epidemiology. Annual Review of Public Health 1: 69-82

Jarczok, K., 1981: Paroxysmal nocturnal hemoglobinuria - a defect of the bone marrow stem cells. Polski Tygodnik Lekarski 36(17): 609-610

Noh, E.Joo.; Jang, E.Ryoung.; Jeong, G.; Lee, Y.Mie.; Min, C.Ki.; Lee, J-Soo., 2005: Methyl CpG-binding domain protein 3 mediates cancer-selective cytotoxicity by histone deacetylase inhibitors via differential transcriptional reprogramming in lung cancer cells. Historic deacetylase inhibitors (HDI) have been reported to inhibit the growth and survival of cancer cells while leaving normal cells untouched. However, the mechanisms underlying this selective cell death are poorly understood. Gene expression a...

Bennett, W.D.; Ivins, S.; Alexis, N.E.; Wu, J.; Bromberg, P.A.; Brar, S.S.; Travlos, G.; London, S.J., 2016: Effect of Obesity on Acute Ozone-Induced Changes in Airway Function, Reactivity, and Inflammation in Adult Females. We previously observed greater ozone-induced lung function decrements in obese than non-obese women. Animal models suggest that obesity enhances ozone-induced airway reactivity and inflammation. In a controlled exposure study, we compared the acut...

Zhao, M.; Yang, L.; Wei, Y.; Xiong, X.; Zhou, Y.; Zhai, S-di., 2011: A case report of monitoring on carbamazepine in breast feeding woman. To evaluate the safety of oral cabamazepine during breast milk feeding. The carbamazepine concentration in breast milk of one epilepsy maternal patient was assayed by high performance liquid chromatography, and the literature was reviewed to find...