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Influence of age on liver phospholipide metabolism of mice

, : Influence of age on liver phospholipide metabolism of mice. Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine 106: 194-196

The influence of age upon radioactive turnover and chromatographic separation of the individual phospholipids was studied in female mice. Radioactive P32 was administered, animals sacrificed, liver removed after 6 hours. From pooled liver homogenates, inorganic soluble P, phospholipid P, N and radioactivity were determined. The phospholipids were chromatographed on silicic acid columns, eluted with chloroform-methanol, and radioactivity and P determined on each fraction. There was an increase in concentration of [mu]g phospholipid P/mg liver N for the 8 to 60 day old mice after which the concentration remained unchanged with age. There was little change in % of total lipid P for the various phospholipid fractions with respect to age. However, the relative specific activities of each phospholipid fraction were greater in the younger mice.


PMID: 13695553

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