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Molecular-genetic polymorphisms of cultivars of common hops (Humulus lupulus L.) using ISSR-PCR analysis

, : Molecular-genetic polymorphisms of cultivars of common hops (Humulus lupulus L.) using ISSR-PCR analysis. Genetika 39(11): 1484-1489

Genetic diversity among 26 Russian and European cultivars of the common hop (Humulus lupulus L.) was studied using the ISSR-PCR technique. Twenty-one primers used provided amplification of 183 DNA fragments, 106 of which (57.9%) were found to be polymorphic. The ISSR markers, specific for certain cultivars were revealed. Based on the coefficient of dissimilarity values, cluster analysis was performed and a dendrogram was constructed, on which most of the hop cultivars formed two clusters according to their origin. Advantages of the ISSR-PCR analysis in selective studies aimed at the classification and identification of common hop cultivars are discussed.


PMID: 14714462

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