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Optical biosensor study of interaction between trypsin and trypsin inhibitor

, : Optical biosensor study of interaction between trypsin and trypsin inhibitor. Biomeditsinskaia Khimiia 51(6): 617-625

The formation of complexes between trypsin (T) and soybean tripsin inhibitor (STI) was analyzed, using a two-channel IAsys+ optical biosensor. The temperature dependence of complex formation (with its major parameters--the association rate constants (k(on)), the dissociation rate constants (k(off)) and the equilibrium constants (Kp)) was determined. The equilibrium constants obtained well correlate with those obtained by other methods. The association rate constant for the binding of T/STI complex increased with temperature, while the dissociation rate constant practically remained unchanged. The association and dissociation rate constants activation parameters were calculated from their concentration dependence. Based on the temperature dependence the activation energy, enthalpy and entropy of complex formation were calculated. It was shown that the entropy component plays a key role in T/STI interaction.


PMID: 16521823

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Cai, L.; Yuan, M-Qing.; Liu, F.; Jian, J.; Chen, G-Qiang., 2009: Enhanced production of medium-chain-length polyhydroxyalkanoates (PHA) by PHA depolymerase knockout mutant of Pseudomonas putida KT2442. Pseudomonas putida KT2442 is a medium-chain-length polyhydroxyalkanoates (PHA) producer. One of the main shortages in the production of PHA has been the intracellular PHA degradation caused by its endogenous PHA depolymerase. The aim of this study...

Tsai, M-Shan.; Jiang, I-Min.; Huang, C-Yen.; Shih, C-Chi., 2003: Reorientational optical nonlinearity of nematic liquid-crystal cells near the nematic-isotropic phase transition temperature. We address the reorientational optical nonlinearity of homogeneously aligned neamtic liquid-crystal (NLC) cells. The propagation of light in NLC cells depend strongly on temperature. At a temperature approaching the clearing point, an undulating b...

Menshutkin V.V.; Golikov A.N., 1971: Simulation of a gastropod mollusk population with the aid of the digital computer. Okeanologiya 11(4): 695-699

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Krasnov, M.M., 1980: New technic of implantation of a modified extrapupillary iris-lens into the posterior chamber before cataract extraction. Vestnik Oftalmologii: 10-13

Gel'man, Z.E.; Tollens, B.C., 1978: Bernhard Tollens and his influence on research into carbohydrates in Russia. Ambix 25(1): 56-62

Carroll, C.H.; Carroll, M.C., 2003: Nebulizing assembly for infants. A nebulizing assembly for infants for delivering inhalable medication to infants and toddlers. The nebulizing assembly for infants includes a mask with a perimeter edge adapted to be positioned such that the user's nose and mouth are within a...

Hughes, S.; Reynolds, B.; Brittain, S.A.; Hudson, J.A.; Freeman, C., 1998: Temporal trends in bromide release following rewetting of a naturally drained gully mire. Recently, there has been interest in the occurrence of bromide (Br-) in natural waters since it has been demonstrated that Br-, in association with humic substances in raw waters, is readily incorporated into haloacetic acids in the form of organi...

Marsh, N.; Askew, D.; Beer, K.; Gerke, M.; Muller, D.; Reichman, C., 1995: Relative contributions of voluntary apnoea, exposure to cold and face immersion in water to diving bradycardia in humans. 1. Diving or face immersion bradycardia is a well recognized but incompletely understood reflex which occurs in man and other mammals. 2. In order to investigate the contributions made by voluntary apnoea, face immersion in water and cold exposure...

Sodomann C.P.; Schmidt M., 1971: In vitro studies on the effect of the migration inhibition factor of lymphocytes on peripheral blood monocytes and granulocytes in man. Medizinische Welt 25: 1062-1063