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Phenotypical determinants of stem cell subpopulations derived from human umbilical cord blood


, : Phenotypical determinants of stem cell subpopulations derived from human umbilical cord blood. Biomedica 26(1): 51-60

Human umbilical cord blood (HUCB) is a common source of hematopoietic progenitor cells; however, coexpression of CD34, CD38 and HLA DR antigens and of embryonic antigens (SSEA-4 and CD13) have not been described in HUCB. The current study attempted to identify in HUCB the presence of these antigens. Cord blood samples were obtained from 80 women with normal pregnancies, who were attending the gynecology-obstetrics service of the San Ignacio Hospital, Bogotá (Colombia). The antigens were identified with a FACS Calibur BDO flow cytometer and a cocktail of monoclonal antibodies. The population of CD34+ cells in HUCB was higher than 0.2% (p = 0.001). The levels of additional cell subpopulations were as follows: CD34+/CD38-/HLA-DR--0.015%-0.023%, CD34+/CD38+/HLA-DR--0.019%-0.30%, CD34+/CD38-/HLA-DR(+)--0.043%-0.068% and CD34+/CD38+/HLA-DR--0.24%-0.51%. Eight samples of the eighty were positive for embryonic markers. The most frequent phenotype in HUCB was CD34+ CD38+ HLA DR+. This is the first time that SSEA-4 and CD13 antigens have been reported in human umbilical cord blood cells.

US$29.90

PMID: 16929903


Other references

Jacobson, L.O., 1954: Modification of radiation injury. Whole-body exposure to ionizing radiations in the lethal or midlethal range produces widespread destruction of certain tissues, e.g., blood-forming tissues, gastro-intestinal tract, and gonads. Death may be related to injury of one or more tissues...

Hatai, K., 1937: A note on the geological significance of the habitat of certain marine invertebrates. Bulletin of the Biogeographical Society Tokyo, 7: 47-55

Yang, D.; Kedei, N.; Li, L.; Tao, J.; Velasquez, J.F.; Michalowski, A.M.; Tóth, Bázs.I.; Marincsák, R.; Varga, A.; Bíró, Tás.; Yuspa, S.H.; Blumberg, P.M., 2010: RasGRP3 contributes to formation and maintenance of the prostate cancer phenotype. RasGRP3 mediates the activation of the Ras signaling pathway that is present in many human cancers. Here, we explored the involvement of RasGRP3 in the formation and maintenance of the prostate cancer phenotype. RasGRP3 expression was elevated in...

Hwang C.A.; Draughon F.A.; Lee B.J.; Loveday H.D.; Riemann M.J., 1991: Effects of ingredients on the survival of campylobacter jejuni in processed turkey ham. Journal of Food Protection 54(10): 817

Waite, R., 1968: The composition of the milk of young and of old cows. In the 1st part of this investigation, 12 cows from the Institute herd were sampled at intervals of 3 wk throughout lactation; of these, 4 were in their 1st lactation, and the others in 6th-llth. In the 2nd part, 4 cows in each of 8 commercial her...

Zhenchevskiĭ, R.A., 1969: Peritoneal injury and the healing of its defects. Eksperimental'naia Khirurgiia i Anesteziologiia 14(6): 19-21

Estell, R.E.; Fredrickson, E.L.; Tellez, M.R.; Havstad, K.M.; Shupe, W.L.; Anderson, D.M.; Remmenga, M.D., 1998: Effects of volatile compounds on consumption of alfalfa pellets by sheep. We examined the effects of six volatile compounds on alfalfa pellet consumption by lambs. In each experiment, 45 lambs were individually fed alfalfa pellets sprayed with a selected compound (camphor, limonene, cis-jasmone, beta-caryophyllene, born...

Gill, S.J.; Gaud, H.T.; Barisas, B.G., 1980: Calorimetric studies of carbon monoxide and inositol hexaphosphate binding to hemoglobin A. Heats of CO and IHP binding to hemoglobin A have been determined under a variety of buffer and pH conditions. From these data heats of ion binding linked to hemoglobin oxygenation have been estimated. For IHP binding to deoxyhemoglobin the buffer-...

Kincheloe, S., 1989: Sul-Po-Magneed/use/value. The product Sul-Po-Mag (potassium magnesium sulfate) is widely used on temperate and tropical crops as a source of available sulfur, magnesium and potassium. This naturally occurring mineral contains 22% K2O, 18% MgO and 22% sulfur, all in the sul...

Montagut, G., 1963: The evolution of nitrogenous fertilizers in soil. Trial on bare soil in Martinique.. Urea, NH4NO3, (NH4)2SO4 or guano was applied at 250 g/m2. The N of plots receiving NH4NO3was most mobile. Urea was rapidly converted to NO3 with only moderate amounts of NH4. Guano gave very little more N than in the control plots. Urea was the be...