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Phosphorylation of Xenopus Cdc25C at Ser285 interferes with ability to activate a DNA damage replication checkpoint in pre-midblastula embryos


, : Phosphorylation of Xenopus Cdc25C at Ser285 interferes with ability to activate a DNA damage replication checkpoint in pre-midblastula embryos. Cell Cycle 2(3): 263-266

We have recently demonstrated that negative regulation of human Cdc25 protein phosphatases by phosphorylation at their 14-3-3 site can be antagonized through phosphorylation at an adjacent site in the -2 position.1 Based on structural homology for different Cdc25 phosphatases, a similar regulatory pathway also could be conserved in Xenopus embryos, where cell cycle checkpoints are not operational prior to the Midblastula Transition (MBT). Here, we demonstrate that before MBT, XeCdc25C is phosphorylated on Ser285, an analogous site to Ser214 in human Cdc25C or Ser307 Cdc25B.(1) Phosphorylation of Ser285 prevents subsequent inhibitory phosphorylation of XeCdc25C on Ser287, thus maintaining XeCdc25C in an active form. Mutation of Ser285 to alanine allows the reconstitution of a DNA damage replication checkpoint. This effect is completely dependent on Ser287 phosphorylation as additional mutation of Ser287 to alanine fully reversed the cell cycle inhibitory effect of Ser285A XeCdc25C. We propose that phosphorylation of XeCdc25C Ser285 may account for the lack of a DNA replication checkpoint in cleaving Xenopus embryos prior to the MBT.

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PMID: 12775939


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Daenen, B.; Preidler, K.W.; Padmanabhan, S.; Brossmann, J.; Tyson, R.; Goodwin, D.W.; Bergman, G.; Resnick, D., 1997: Symptomatic herniation pits of the femoral neck: anatomic and clinical study. The purpose of this study was to investigate the pathogenesis of herniation pits of the femoral neck, which are frequently considered a normal variant, and to describe the imaging abnormalities of such pits in three symptomatic patients. The chang...

Sanaee Jahromi, S.; Depeweg, H.; Feyen, J., 2000: Water delivery performance in the Doroodzan irrigation scheme, Iran. Delivery performance ratio was used to assess the water delivery performance in an irrigation district in the Doroodzan Irrigation System in Iran. The measurements were applied to three selected irrigation canals and their tertiary outlets during...

Yadav, B.S.; Khan, F.H.; Yadav, M.C., 1995: Genetic studies on persistency of first lactation in murrah buffaloes. Indian Veterinary Medical Journal 19(4): 291-292

Clyde, W.A., 1968: An experimental model for human mycoplasma disease. Yale Journal of Biology and Medicine 40(5-6): 436-443

Hogan, M.J.; Kimura, S.J.; Lewis, A.; Zweigart, P.A., 1957: Early and delayed ocu lar manifestations of congenital toxoplasmosis. Transactions of the American Ophthalmological Society 55: 275-93; Discussion 293-6

Block, B.M.; Jones, S.W., 1997: Delayed rectifier current of bullfrog sympathetic neurons: ion-ion competition, asymmetrical block and effects of ions on gating. 1. The delayed rectifier (DR) K+ channel pore was probed using different permeant and blocking ions applied intra- and extracellularly. Currents were recorded from bullfrog sympathetic neurons using whole-cell patch-clamp techniques. 2. With intra...

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Wilson, B.D.; Lippmann, M.L., 1993: Amiodarone pulmonary toxicity in the rat is associated with increased lavage immunoglobulin and alveolar macrophages primed for increased interleukin-1 secretion. To determine whether a hypersensitivity reaction played a role in amiodarone-induced pulmonary inflammation and affected humoral immunity, we examined pulmonary immunoglobulin (Ig) levels, alveolar macrophage release of interleukin-1 (IL-1)-like a...

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