geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Prolonged storage effects on the articular cartilage of fresh human osteochondral allografts


, : Prolonged storage effects on the articular cartilage of fresh human osteochondral allografts. Journal of Bone and Joint Surgery. American Volume 85-A(11): 2111-2120

Fresh osteochondral allograft transplantation is a well-established technique for the treatment of cartilage defects of the knee. It is believed that the basic paradigm of the technique is that the transplantation of viable chondrocytes maintains the articular cartilage matrix over time. Allograft tissue is typically transplanted up to forty-two days after the death of the donor, but it is unknown how the conditions and duration of storage affect the properties of fresh human osteochondral allografts. This study examined the quality of human allograft cartilage as a function of storage for a duration of one, seven, fourteen, and twenty-eight days. We hypothesized that chondrocyte viability, chondrocyte metabolic activity, and the biochemical and biomechanical properties of articular cartilage would remain unchanged after storage for twenty-eight days. Sixty osteochondral plugs were harvested from ten fresh human femoral condyles within forty-eight hours after the death of the donor and were stored in culture medium at 4 degrees C. At one, seven, fourteen, and twenty-eight days after harvest, the osteochondral plugs were analyzed for (1) viability and viable cell density by confocal microscopy, (2) proteoglycan synthesis by quantification of (35)SO(4) incorporation, (3) glycosaminoglycan content, (4) indentation stiffness, (5) compressive modulus and hydraulic permeability by static and dynamic compression testing, and (6) tensile modulus by equilibrium tensile testing. Chondrocyte viability and viable cell density remained unchanged after storage for seven and fourteen days (p > 0.7) and then declined at twenty-eight days (p < 0.001). Proteoglycan synthesis remained unchanged at seven days (p > 0.1) and then declined at fourteen days (p < 0.01) and twenty-eight days (p < 0.001). No significant differences were detected in glycosaminoglycan content (p > 0.8), indentation stiffness (p > 0.4), compressive modulus (p > 0.05), permeability (p > 0.3), or equilibrium tensile modulus after storage for twenty-eight days (p > 0.9). These data demonstrate that fresh human osteochondral allograft tissue stored for more than fourteen days undergoes significant decreases in chondrocyte viability, viable cell density, and metabolic activity, with preservation of glycosaminoglycan content and biomechanical properties. The cartilage matrix is preserved during storage for twenty-eight days, but the chondrocytes necessary to maintain the matrix after transplantation decreased over that time-period.

US$29.90

PMID: 14630839


Other references

Ahmad, S.; Seshadri, M., 2005: A hemizygous repeat polymorphism of one STR in four population groups of South India. Journal of Forensic Sciences 50(6): 1489-1490

Rossi J.J.; IImura Y., 1984: Fluctuations in the pattern of expression of yeast actin escherichia coli lac z gene fusions. Journal of Cellular Biochemistry Supplement (8 PART B): 145

Lee R S.; Mcdonagh P.F., 1985: The effect of serotonin on pial microvascular tone. Federation Proceedings 44(3): 433

Gu, Y.; Chen, X.; Liu, Z.; Zhou, X.; Zhang, Y., 2014: Effect of inoculum sources on the anaerobic digestion of rice straw. The aim of this study was to evaluate the effect of different inoculum sources on the rice straw anaerobic digestion. Six different digestates (DM, SM, CM, MS, AGS and PS) were applied as inoculums and their effects were evaluated in batch reactor...

Arita, T.; Miyazaki, K., 1987: Intestinal absorption and the mechanism of ionized drugs. Yakugaku Zasshi 107(4): 249-261

Stranlund, J.K.; Ben-Haim, Y., 2007: Price-based vs. quantity-based environmental regulation under Knightian uncertainty: an info-gap robust satisficing perspective. Conventional wisdom among environmental economists is that the relative slopes of the marginal social benefit and marginal social cost functions determine whether a price-based or quantity-based environmental regulation leads to higher expected s...

Dolan, T.A.; Peek, C.W.; Stuck, A.E.; Beck, J.C., 1998: Three-year changes in global oral health rating by elderly dentate adults. The single item global rating is an economical way of summarizing the state of a person's oral health, and thus is a potentially valuable oral health outcome measure. This research describes the nature, magnitude, and direction of changes in...

Zhou, M.; Gu, L.; Yeager, A.M.; Findley, H.W., 1998: Expression and function of Fas in acute lymphoblastic leukemia cell lines with p53 mutation. Proceedings of the American Association for Cancer Research Annual Meeting 39: 581, March

Shoji, F.; Yamashita, N.; Inoue, Y.; Kozuma, Y.; Toyokawa, G.; Hirai, F.; Ito, K.; Tagawa, T.; Okamoto, T.; Maehara, Y., 2017: Surgical Resection and Outcome of Synchronous and Metachronous Primary Lung Cancer in Breast Cancer Patients. Label="BACKGROUND/AIM">Women with breast cancer are at increased risk of subsequent primary malignancies, specifically lung cancer. The aim of this study was to report the frequency of lung cancer in patients with breast cancer, and p...

Campos, A.; Amor, A.; González, Mía.Angeles., 2002: Presentation of keywords by means of interactive drawings. One of the main outstanding problems in keyword mnemotechnics is whether this technique is more effective when the subjects generate their own keywords, or when the keywords are supplied by the experimenter. Both methods have advantages and disadv...