geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Prooxidant action of rhodizonic acid: transition metal-dependent generation of reactive oxygen species causing the formation of 8-hydroxy-2'-deoxyguanosine formation in DNA


, : Prooxidant action of rhodizonic acid: transition metal-dependent generation of reactive oxygen species causing the formation of 8-hydroxy-2'-deoxyguanosine formation in DNA. Toxicology in Vitro 20(6): 910-914

Rhodizonic acid, a six-membered cyclic hydroxyquinone, produced reactive oxygen species as a complex with transition metals. Addition of rhodizonic acid with ferrous ion caused an inactivation of aconitase the most sensitive enzyme to oxidative stress in permeabilized yeast cells. The iron-dependent inactivation of aconitase implies the rhodizonic acid/iron-mediated generation of reactive oxygen species. Spectrophotometric analysis of the interaction of rhodizonic acid with FeSO4 showed that addition of superoxide dismutase could inhibit the oxidation of rhodizonic acid, suggesting that reactive oxygen species produced from rhodizonic acid is superoxide radical. Rhodizonic acid further acted as a prooxidant causing a copper-dependent DNA damage. Treatment of DNA from plasmid pBR322 and calf thymus with rhodizonic acid plus copper caused strand scission and the formation of 8-hydroxy-2'-deoxyguanosine in DNA. Addition of catalase protected DNA from the rhodizonic acid-mediated strand scission, indicating that hydroxyl radical may participate in the DNA damage. Rhodizonic acid also showed a potent copper-reducing activity. These results indicate that copper ion reduced by rhodizonic acid may participate in the formation of superoxide radical that converts to hydrogen peroxide and hydroxyl radical. Other cyclic hydroxyquinones such as four-membered squaric acid and five-membered croconic acid did not show any prooxidant and reducing effects. Cytotoxic effects of tetrahydroquinone the precursor of rhodizonic acid may be related to the prooxidant properties of rhodizonic acid formed in cells.

US$19.90

PMID: 16504460

DOI: 10.1016/j.tiv.2006.01.009


Other references

D.K.oon H.; Kwant R.; Hara T., 1991: Density dependent growth responses in two clonal herbs the influence of physiological integration. Bulletin of the Ecological Society of America 72(2 SUPPL): 98

Blazek J.; Syka J., 1978: Indirect determination of packing density and proximity of particles and non-clastic minerals in sediments. Casopis pro Mineralogii a Geologii 23(3): 225-232

Doucet, I.; Filipescu, H., 1974: Breeding for oil content and quality in linseed. Work carried out on the inheritance of quality characters and methods of breeding for quality is summarized. Analysis of 126 F6 hybrids in Romania revealed 69% with 1.36% more oil than the control. Lines with oil contents 2-27%, 8-31%, 11-19% and...

Delben, J.Aparecida.; Goiato, M.Coelho.; Pellizzer, E.Piza.; Magro Filho, O., 2013: Planning for immediate loading of implant-supported prostheses: literature review. The aim of this study was to present the factors that influence planning for immediate loading of implants through a literature review for treatment success. Research was conducted in the PubMed database including the key words immediate implant l...

Simons, M.D., 1985: Transfer of field resistance to Puccinia coronata from Avena sterilis to cultivated oats by backcrossing. Fourteen A. sterilis strs., all lacking seedling resistance to P. coronata, were crossed and backcrossed once and twice with the susceptible cv. Clinton. Lines derived from F1, BC1 and BC2 plants were selected for cultivated plant type and exposed...

Ten Have, M.; Lamers, F.; Wardenaar, K.; Beekman, A.; de Jonge, P.; van Dorsselaer, S.; Tuithof, M.; Kleinjan, M.; de Graaf, R., 2016: The identification of symptom-based subtypes of depression: A nationally representative cohort study. In recent years, researchers have used various techniques to elucidate the heterogeneity in depressive symptoms. This study seeks to resolve the extent to which variations in depression reflect qualitative differences between symptom categories an...

Kariev, T.M.; Ibragimov, M.A., 1983: Lobectomy in patients with diffuse forms of pulmonary tuberculosis. Grudnaia Khirurgiia: 45-47

Lau, O.-Wah; Luk, S.-Fai, 1994: Spectrophotometric method for the determination of ethanol in beverages and beer samples using cerium (IV) as reagent. A simple and rapid (15 min) spectrophotometric method for the determination of subpercentage ethanol in beverages is based on the colour reaction between ethanol and Ce(IV). Other alcohols and reducing substances interfere, but may be minimized by...

Roella, C.; Falaschi, F., 1960: New orientations in practical cardiotherapy. The results of an associated coronarydilator-coenzyme-vitamin therapy. Minerva Cardioangiologica 8: 399-410

V.I.Burnashev, B.A.Burnasheva, 2013: Absolute spectrophotometry of 22 vulpeculae during the eclipse of 1990. The results of broadband photometric observations of 22 Vulpeculae (QS Vulpeculae) between 1984 and 1994 have been considered and unified to produce a composite light curve. The spectral types of the binary components and their contributions to th...