geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Proteins interacting with Saccharomyces cerevisiae type 1 protein phosphatase catalytic subunit identified by single-step affinity purification and mass spectrometry


, : Proteins interacting with Saccharomyces cerevisiae type 1 protein phosphatase catalytic subunit identified by single-step affinity purification and mass spectrometry. Methods in Molecular Biology 365: 235-246

The catalytic subunit of type 1 protein phosphatase (PP1C) interacts with a large number of polypeptides in eukaryotic cells from yeast to man and these regulatory subunits can both modulate the activity of PP1C and target it to different subcellular locations. Thus, PP1 is really a family of protein phosphatases that share a common catalytic subunit, and identifying and characterizing the PP1-associated proteins is therefore critical to understanding the cellular roles of PP1 and its ability to dephosphorylate specific substrates. Here we describe methods for affinity isolation of PP1C-containing protein complexes in the yeast Saccharomyces cerevisiae and the identification of the associated polypeptides by mass spectrometry. The basic method we describe could be easily adapted to study PP1C-associated proteins in other lower or higher eukaryotes and for characterizing the protein-protein interactions of other protein phosphatases in yeast.

US$19.90

PMID: 17200566

DOI: 10.1385/1-59745-267-X:235


Other references

Williams M.E.; Connolly N.K., 1990: What practicing physicians in north carolina usa rate as their most challenging geriatric medicine concerns. We recently surveyed a random sample of 500 physicians in family practice, general practice, and internal medicine in North Carolina, to discover their most challenging geriatric concerns. Using a three-stage survey technique, respondents were ask...

Yafei, C.; Yong, Z.; Xiaoming, Z.; Peng, G.; Hailong, A.; Yuguang, D.; Yingrong, H.; Hui, L.; Yuhong, Z., 2009: Functions of oligochitosan induced protein kinase in tobacco mosaic virus resistance and pathogenesis related proteins in tobacco. Oligochitosan (OC) can regulate plant defense responses in many aspects, but the basic signal transduction pathway is still unclear. In this study, we used transgenic (TG) tobacco (Nicotiana Tabacum var. Samsun NN) as plant material whose oligochi...

Mine, T.; Kataoka, A.; Fujisaki, J.; Sato, E.; Yasuda, H.; Inaba, Y.; Akimoto, K.; Mashima, H.; Ogata, E., 1994: Healing of chronic gastric ulcer depends on gastric mucosal prostaglandin synthesis. In the present study, the role of endogenous prostanoid synthesis in gastric mucosa in the healing of chronic gastric ulcers was investigated. Nineteen patients were divided into two groups in accordance with healing state after one month of treat...

Cochran, P.A., 1983: Life history notes. Sauria. Eumeces septentrionalis (northern prairie skink). Agonistic behaviour. Herpetological Review 14.4: 119

Keoghan, J.M.; Tossell, W.E., 1974: Forage yield potential of contrasting birdsfoot trefoil genotypes. There were significant genotype differences in herbage yield and yield components in each regrowth and for the whole season when contrasting Lotus corniculatus genotypes and Medicago sativa were grown under high-plant density, simulated-sward cond...

Ponassi, A.; Morra, L.; Caristo, G.; Parodi, G.B.; Biassoni, P.; Sacchetti, C., 1983: Disorders of granulopoiesis in patients with untreated Graves' disease. Total and differential leukocyte countings, mobilization of the marrow granulocyte reserve with hydrocortisone and growth of blood colony-forming cells (CFU-C) in agar culture have been evaluated in 17 patients with Graves' disease before the...

Anonymous, 1983: Bins & tote boxes. Boxes and bins are replaceable items necessary to the foodservice industry. Food or tote boxes are used to convey food or dirty dishes. Tote boxes don't require lids, save labor by reducing transport, and require replacement often. Tote boxes...

Centron, D.; Roy, P.H., 1997: Analysis of a Serratia marcescens strain that harbors three integrons. Abstracts of the General Meeting of the American Society for Microbiology 97(0): 295

Blasco-Lafarga, C.; Martínez-Navarro, I.; Mateo-March, M., 2014: Is baseline cardiac autonomic modulation related to performance and physiological responses following a supramaximal Judo test?. Little research exists concerning Heart Rate (HR) Variability (HRV) following supramaximal efforts focused on upper-body explosive strength-endurance. Since they may be very demanding, it seems of interest to analyse the relationship among perform...

Milne, J.L.; Coukell, M.B., 1988: Isolation and characterization of a plasma membrane calcium pump from Dictyostelium discoideum. During the aggregation and differentiation of amoebae of Dictyostelium discoideum, changes in free cytosolic Ca2+ appear to regulate a number of physiological processes. To understand the mechanisms regulating free intracellular Ca2+ in this organ...