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Proteome analysis for the identification of in vivo estrogen-regulated proteins in bone

, : Proteome analysis for the identification of in vivo estrogen-regulated proteins in bone. Proteomics 5(18): 4936-4945

Estrogen deficiency results in a reduced bone mass, which can be prevented by treatment with estrogens. This study used a proteomic approach for the first time to obtain a global perspective of estrogens' effects on whole-bone proteins. Bone proteome profiles were examined in three groups of mice: (1) sham-operated with normal ovarian functions, (2) ovariectomised and (3) ovariectomised with estrogen replacement therapy. Bone proteins extracted from the humerus were separated by 2-DE and visualised by CBB colloidal staining. Spot detection and quantification was done by image analysis. Differentially expressed proteins were identified by MS and database search, using peptide mass fingerprint and peptide sequence analysis. Differential expression analysis in the three experimental groups showed significant changes for 14 proteins. These included proteins related to bone metabolism, cytoskeleton components and energy metabolic pathways. Our data suggest that some proteins related to cytoskeleton and to energy pathways, such as tropomyosins, aconitase 2 and enolase beta, might be new molecular targets responsive to the effects of estrogen. Differentially expressed proteins identified in this model may offer a useful starting point for elucidating novel aspects of the pleiotropic effects of estrogens on bone.


PMID: 16237733

DOI: 10.1002/pmic.200401325

Other references

Pense, A.M.; Huchon Becel, D., 1993: Resistance to antibiotics in a gerontological center. Journal de Pharmacie Clinique 12(3): 209-213

Riley, M.Beth.; Byar, K., 2004: The rationale for and background of radioimmunotherapy: an emerging therapy for B-cell non-Hodgkin's lymphoma. To present the rationale for and development of radioimmunotherapy (RIT) for non-Hodgkin's lymphoma (NHL), which culminated in the FDA's approval of yttrium 90 (90Y) ibritumomab tiuxetan in February 2002. Published clinical trials of 90Y...

Held, Hans., 1909: Untersuchungen uber den feineren Bau des Ohrlabyrinthes der Wirbeltiere. II. Zur Entwicklungs-geschichte des Cortischen Organs und der Macula acustica bei Saugetieren und Vogeln. Leipzig Abh Ges Wiss math phys Kl, 31 193-294

Katz, D.; Rosenberger, R.F., 1970: The utilisation of galactose by an Aspergillus nidulans mutant lacking galactose phosphate-UDP glucose transferase and its relation to cell wall synthesis. Archiv für Mikrobiologie 74(1): 41-51

Strange, H.H., 1953: Morphology of the fimbria ovarica. Zentralblatt für Gynakologie 75(36): 1401-1412

Sanderson, I.R.; Boyle, S.; Williams, C.B.; Walker-Smith, J.A., 1986: Histological abnormalities in biopsies from macroscopically normal colonoscopies. One hundred and nineteen children with gastrointestinal symptoms underwent colonoscopy during the course of one year. Out of 39 children that were reported as yielding normal results on endoscopic examination, six had disease detectable only by hi...

Pilsbry, H., 1930: A Giffordius pinchoti n g, n sp G corneliae n sp. Nautilus Boston Mass, 43: 142-143

Anonymous, 2016: Hygienic Evaluation Of Territories Of Fergana Valley Recreational Areas. The article is devoted to hygienic monitoring ofrecreational areas of Fergana Valley. The aim is a hygienic evaluation of the Fergana Valley's (FV) mountain green areas on the basis of studies of heavy metals content in soil and indices of th...

Hacek, D.M.; Paule, S.; Small, M.; Gottschall, R.; Thomson, R.; Peterson, L., 2003: Comparison of colistin naladixic acid , mannitol salt agar and phenol mannitol broth with antibiotics for the recovery of Staphylococcus aureus from nasal swabs. Background: Accurate detection of methicillin-resistant S. aureus (MRSA) nasal colonization is needed for infection control programs to reduce MRSA transmission, and of all SA for patient decolonization as part of programs to lower post-surgical o...

Glomb, V., 1980: Arboricidal effectiveness of some preparations. Sbornik Vedeckeho lesnickeho ustavu Vysoke skoly zemedelske v Praze: 3) 71-87