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Quantification of stratum corneum removal by adhesive tape stripping by total protein assay in 96-well microplates

, : Quantification of stratum corneum removal by adhesive tape stripping by total protein assay in 96-well microplates. Skin Research and Technology 11(2): 97-101

Examination of stratum corneum (SC) content with tape stripping and a colorimetric method is increasingly used. We examined the possible use of microplates in tandem with a colorimetric method to examine SC removed with tape stripping. As a corollary to this examination, the homogeneity of tape strips was examined. The commonly used Lowry assay was adapted for 96-well plates. Tapes were divided into four regions and sample disks of 5 mm diameter were taken from each and analyzed for SC mass using the adapted Lowry assay. Homogeneity of SC removal over different areas across a tape strip is limited. Quantification of SC by means of a 96-well microplate-based colorimetric method is feasible and shortens the time of analysis. However, when using D-Squame tape disks, SC removal on a limited area of the tape is not predictive for SC removal on the entire tape as removal is inhomogenous. Therefore, SC protein extraction should be performed on a large enough area, eventually on the entire tape when quantifying SC mass removed by tape stripping.


PMID: 15807806

DOI: 10.1111/j.1600-0846.2005.00103.x

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