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Reovirus outer capsid protein micro1 induces apoptosis and associates with lipid droplets, endoplasmic reticulum, and mitochondria

, : Reovirus outer capsid protein micro1 induces apoptosis and associates with lipid droplets, endoplasmic reticulum, and mitochondria. Journal of Virology 80(17): 8422-8438

The mechanisms by which reoviruses induce apoptosis have not been fully elucidated. Earlier studies identified the mammalian reovirus S1 and M2 genes as determinants of apoptosis induction. However, no published results have demonstrated the capacities of the proteins encoded by these genes to induce apoptosis, either independently or in combination, in the absence of reovirus infection. Here we report that the mammalian reovirus micro1 protein, encoded by the M2 gene, was sufficient to induce apoptosis in transfected cells. We also found that micro1 localized to lipid droplets, endoplasmic reticulum, and mitochondria in both transfected cells and infected cells. Two small regions encompassing amphipathic alpha-helices within a carboxyl-terminal portion of micro1 were necessary for efficient induction of apoptosis and association with lipid droplets, endoplasmic reticulum, and mitochondria in transfected cells. Induction of apoptosis by micro1 and its association with lipid droplets and intracellular membranes in transfected cells were abrogated when micro1 was coexpressed with sigma3, with which it is known to coassemble. We propose that micro1 plays a direct role in the induction of apoptosis in infected cells and that this property may relate to the capacity of micro1 to associate with intracellular membranes. Moreover, during reovirus infection, association with sigma3 may regulate apoptosis induction by micro1.


PMID: 16912293

DOI: 10.1128/JVI.02601-05

Other references

Main; Wiebusch; Holle; Welge, 1986: New quasi-Landau structure of highly excited atoms: The hydrogen atom. Physical Review Letters 57(22): 2789-2792

Watters, V.A.; Grant, J.P., 1997: Use of electrocardiogram to position right atrial catheters during surgery. This study evaluated the accuracy of placing right atrial catheters using an electrocardiographic (ECG) technique. Placement of right atrial catheters for vascular access is a common operative procedure. Accurate placement is essential for proper...

Guthrie, P.B.; Neuhoff, V.; Osborne, N.N., 1975: Dopamine, noradrenaline, octopamine and tyrosine-hydroxylase in the gastropod Helix pomatia. Comparative Biochemistry and Physiology. C: Comparative Pharmacology 52(2): 109-111

Elia, M., 2001: Measurement of total body water in patients with short-bowel syndrome. Nutrition 17(5): 424-425

Paris Jack F., 1974: Coastal zone mapping from ERTS-1 data using computer-aided techniques. Pages 515-528 1974

Wolford, E.R., 1955: Significance of the presumptive coliform test as applied to orange juice. Approximately 76% of the soluble solids of orange juice is carbohydrate consisting of sucrose, dextrose and levulose. When lactose broth is inoculated with orange juice 2/3 of the sugars are other than lactose, and gas in such tubes could have res...

Klostermeyer, E.C.; Rasmussen, W., 1956: A counting plate for sampling mite populations. A simplified method for determining mite populations on herbaceous crops is described using a shaker to separate insect from mite populations and a counting plate with cross strips for aliquoting the sample.

Koh, T.W.; Hooper, J.; Kemp, M.; Ferdinand, F.D.; Gibson, D.G.; Pepper, J.R., 1999: Intraoperative release of troponin T in coronary venous and arterial blood and its relation to recovery of left ventricular function and oxidative metabolism following coronary artery surgery. To investigate the intraoperative release of troponin T during uncomplicated coronary artery surgery and to determine its relation to ischaemic time and to recovery of left ventricular function and oxidative metabolism. A prospective observational...

Santa Teresa I.; Carbo A.; Capote R.; Casquet C., 1983: Geometry of the depths of the Orgaz Granite from gravimetric data. Studia Geologica, Universidad de Salamanca 18: 237-250

Fletcher, S.M.; Stephens, D.M., 1987: An evaluation of anti-A and anti-B monoclonal antibodies for ELISA grouping of blood and body fluid stains. Revue Francaise de Transfusion et Immuno-Hematologie 30(5): 421-428