geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Study on the interaction of bovine serum albumin with bamphenol blue in acidic solution by spectrophotometry


, : Study on the interaction of bovine serum albumin with bamphenol blue in acidic solution by spectrophotometry. Guang Pu Xue Yu Guang Pu Fen Xi 23(2): 342-344

On the basis of the difference between the absorption spectra of free and bound BPB, the interaction of bamphenol blue (BPB) with bovine serum albumin (BSA) in acidic solution was investigated by a spectrophotometric method. Solutions containing 5.0 x 10(-6) mol.L-1 of dye concentration and various BSA concentrations from 0.5 x 10(-6) to 3.8 x 10(-6) mol.L-1 were prepared in pH 2.79 Britton-robinson buffer, and the absorbances were measured at 605 nm. The result calculated from the above experiments show that the ratio between the average number of dye molecules bound per protein molecule and the concentration of free BPB is approximately constant. So the interaction of BSA and BPB in the experimental conditions selected in this paper can be regarded as a kind of phase distribution. A simple double reciprocal equation for the estimation of apparent binding constant and the maximum binding number was easily derived. The linear regression between the reciprocal of the observed absorbance change at 605 nm due to the addition of BSA to a fixed quantity of BPB (delta A-1) and the reciprocal of the concentration of BSA (CP-1) was made. All of the regression coefficients for the lines of delta A-1 versus CP-1 obtained from various pH values, BPB concentrations and ion strengths were better than 0.99. Through the ratio of intercept and slop, both the apparent binding constants and the maximum binding numbers were calculated for various experimental conditions.

US$29.90

PMID: 12961890


Other references

Borden J.H.; Devlin D.R.; Miller D.R., 1992: Synomones of sympatric species deter attack by the pine engraver ips pini coleoptera scolytidae. The capture of pine engravers, Ips pini (Say), in ipdienol-baited, multiple-funnel traps in British Columbia [Canada] was significantly reduced when devices releasing ipsenol or verbenone were placed in the traps. These results suggest that ipseno...

Hayashi, Y.; Akiyama, S.; Miyaji, A.; Sekiguchi, Y.; Sakamoto, Y.; Shiga, A.; Koyama, T-Ru.; Motokura, K.; Baba, T., 2016: Experimental and computational studies of the roles of MgO and Zn in talc for the selective formation of 1,3-butadiene in the conversion of ethanol. NlmCategory="UNASSIGNED">The one-step conversion of ethanol to 1,3-butadiene was performed using talc containing Zn (talc/Zn) as a catalyst. The influence of the MgO and Zn in the talc on the formation rate and selectivity for 1,3-but...

Koller, B.; Neuhann, T.F.; Neuhann, I.M., 2014: Keratoplasty in patients with intellectual disability. The aim was to report the outcome of keratoplasty in patients with intellectual disability (ID). All patients with ID who had undergone keratoplasty at the Eye Department, Red Cross Hospital, Munich, between January 1, 1991, and December 31, 2010,...

Bao, W.; Pan, F.; Chen, L.; Su, G.; Gao, X.; Li, Y.; Sun, Q.; Sun, J.; He, K.; Song, H., 2014: The PI3K/AKT Pathway and FOXO3a Transcription Factor Mediate High Glucose-Induced Apoptosis in Neonatal Rat Ventricular Myocytes. PI3K/AKT pathway plays major roles in regulating cardiomyocyte metabolism. The roles of PI3K/AKT pathway and FOXO3a in mediating high glucose-induced apoptosis in cardiomyocytes remain unclear. In this experimental study, we investigated the mecha...

Prizant, E.; Kogut, M., 1975: In vivo and in vitro binding of dihydrostreptomycin to Escherichia coli ribosomes. Febs Letters 50(1): 37-42

Ghilarov, M.S.; Semenova, L.M., 1978: Digestive system of soil insect larvae with different feeding habits. A histological comparison of the digestive tracts of many soil-dwelling species of saprophagous, phytophagous and predacious insect larvae in the laboratory in the USSR showed that the structure and texture of the intestine is adapted to the feedi...

Takaishi M.; Sadamoto K.; Takami S.; Ishioka S.; Hozawa S.; Yamakido M., 1990: Changes of various serum protein levels between acute and convalescent phases in patients with respiratory infections. We determined serum levels of .alpha.1-antitrypsin, .alpha.1-acid glycoprotein, ceruloplasmin, haptoglobin, .alpha.2-HS glycoprotein, C3 and fibronectin in 22 patients with acute pneumonia, 10 patients with mycoplasma pneumonia and 8 patients with...

Torre, P.; Dreisbach, L.E.; Kopke, R.; Jackson, R.; Balough, B., 2008: Risk factors for distortion product otoacoustic emissions in young men with normal hearing. The purpose of this study was to evaluate the possible effects of risk factors on distortion product otoacoustic emissions (DPOAEs) in young adult men with normal hearing. Four hundred thirty-six United States Marine recruit men (mean age = 19.2 y...

Ozkan, Y.; Savaşer, A.; Ozalp, Y.; Işimer, A., 2000: Dissolution properties of different designed and formulated salbutamol tablet dosage forms. Pharmaceutical availability or in vitro availability is one of the aspects of drug bioavailability. Dissolution can be described best as a tool that can provide valuable information about the availability of a drug product. Dissolution test was pe...

Bahouth, S.W.; Sowinski, K.M.; Lima, J.J., 2001: Regulation of human beta(1)-adrenergic receptors and their mRNA in neuroepithelioma SK-N-MC cells: effects of agonist, forskolin, and protein kinase A. We determined the effect of long-term exposure to beta-agonists on beta(1)-adrenergic receptors (beta(1)-AR) in human neuroepithelioma SK-N-MC cells because earlier studies have indicated that beta(1)-AR in this cell line are resistant to agonist-...