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Targeting of hematopoietic progenitor cells with MR contrast agents

, : Targeting of hematopoietic progenitor cells with MR contrast agents. Radiology 228(3): 760-767

To label human hematopoietic progenitor cells with various magnetic resonance (MR) imaging contrast agents and to obtain 1.5-T MR images of them. Hematopoietic progenitor cells, labeled with ferumoxides, ferumoxtran, magnetic polysaccharide nanoparticles-transferrin, P7228 liposomes, and gadopentetate dimeglumine liposomes underwent MR imaging with T1- and T2-weighted spin-echo and fast field-echo sequences. Data were analyzed by measuring MR signal intensities and R1 and R2* relaxation rates of labeled cells and nonlabeled control cells. Mean quantitative data for the various contrast agent groups were assessed for significant differences compared with control cells by means of the Scheffe test. As a standard of reference, MR imaging data were compared with electron microscopic and spectrometric data. For all contrast agents, intracellular cytoplasm uptake was demonstrated with electron microscopy and was quantified with spectrometry. When compared with nonlabeled control cells, progenitor cells labeled with iron oxides showed significantly (P <.05) increased R2*. Cells labeled with gadopentetate dimeglumine liposomes showed significantly increased R1. Detection thresholds were 5 x 10(5) cells for gadopentetate dimeglumine liposomes and ferumoxtran, 2.5 x 10(5) cells for ferumoxides and P7228 liposomes, and 1 x 10(5) cells for magnetic polysaccharide nanoparticles-transferrin. Hematopoietic progenitor cells can be labeled with MR contrast agents and can be depicted with a standard 1.5-T MR imager.


PMID: 12881578

DOI: 10.1148/radiol.2283020322

Other references

Ennis, H.L., 1971: Interaction of vernamycin A with Escherichia coli ribosomes. Biochemistry 10(7): 1265-1270

Jordan, R.E.; Nakada, M.T.; Mascelli, M.A.; Mace, K.F.; Nedelman, M.A.; Barnathan, E.S.; Weisman, H.F., 1998: Abciximab affords early and sustained reperfusion by reduced-dose reteplase in a primate model of severe arterial injury. Circulation 98(17 SUPPL ): I526, Oct 27

Haruki, N.; Tsang, W.; Thavendiranathan, P.; Woo, A.; Tomlinson, G.; Logan, A.G.; Bradley, T.Douglas.; Floras, J.S., 2016: Sleep Apnea and Left Atrial Phasic Function in Heart Failure With Reduced Ejection Fraction. The study aim was to determine whether phasic left atrial (LA) function of patients with heart failure with reduced ejection fraction differs between those with obstructive sleep apnea (OSA) and central sleep apnea (CSA). Participation in the Adap...

Qu, L.; Tian, X.; Wang, C.; Sun, C.; Chen, J., 2016: Salinovum rubellum gen. nov., sp. nov., isolated from sediment of a saltern. An aerobic, Gram-reaction-negative, oxidase-positive and catalase-positive strain, YCWB25T, was isolated from saltern farm sediment in Jimo-Daqiao of Qingdao, east coast of China. This strain had an absolute requirement for NaCl and was able to gr...

Wright, C.I.; McMullin, K.; Martis, B.; Fischer, Håkan.; Rauch, S.L., 2005: Brain correlates of negative visuospatial priming in healthy children. Inhibitory mechanisms that begin to develop in childhood are essential for efficient and goal-directed behaviors. These inhibitory mechanisms may go awry in several childhood-onset neuropsychiatric disorders, such as Tourette syndrome, obsessive-c...

Haas, H.; Dorfman, M.L.; Sacks, T.G., 1971: T-strains of mycoplasma and nongonococcal urethritis. British Journal of Venereal Diseases 47(2): 131-134

Christopher, M.M.; Harvey, J.W., 1992: Specialized hematology tests. In conclusion, many specialized tests for the evaluation of anemia and RBC abnormalities can readily be performed in-house or by commercial laboratories. Tests include RBC indices, examination of blood smears (for morphological changes, infectious...

Winkler, S.R.; Munoz-Ruiz, L., 1995: Mechanism of action of mannitol. Surgical Neurology 43(1): 59-59

Kaulenas, Ms, 1971: Cold-induced run-off ribosomes of Acheta domesticus. Cricket “run-off” ribosomes have a lower Mg2+ optimum and are approximately six times more active than other monomers in poly U-directed protein synthesis. Both differences can be eliminated by washing “run-off” ribosomes with 0.5 M NH4Cl....

Rilov, G.; Crowder, L.B.; Mcclellan, C.M., 2001: Ecological effects of the hurricanes of 1999 Spatial and temporal responses of the Pamlico Sound fish community. Ecological Society of America Annual Meeting Abstracts 86: 190