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Taxonomic relationship among seven species of groupers (genus Epinephelus; family Serranidae) as revealed by RAPD fingerprinting

, : Taxonomic relationship among seven species of groupers (genus Epinephelus; family Serranidae) as revealed by RAPD fingerprinting. Marine Biotechnology 6(3): 229-237

Phenotypic identification of groupers of the genus Epinephelus is based on color patterns and a suite of morphologic characters. However, these characters often show intraspecific variations and differences between juveniles and adults of the same species. The present work is an attempt to study and ratify the status of Epinephelus spp. using random amplified polymorphic DNA (RAPD) analysis based on samples drawn from southeast and southwest coasts of India. The RAPD fingerprints generated in Epinephelus diacanthus, E. areolatus, E. chlorostigma, E. bleekeri, E. coioides, E. tauvina, and E. malabaricus with 4 primers (OPA 01, OPA 07, OPF 08, and OPF 10) were consistent, reproducible, and yielded species-specific diagnostic markers in all the species. A total of 59 RAPD loci in the size range of 70 to 4500 bp were produced from all 4 arbitrary primers. An unweighted pair-group method with arithmetic mean (UPGMA) dendogram was constructed based on genetic distance values to show the genetic relationships among the 7 species. All the individuals of each species formed monophyletic species clusters. The mean intraspecies genetic distance value (0.305) was significantly lower than the interspecies value (0.365). Epinephelus malabaricus was most distantly related to E. diacanthus and E. bleekeri. The genetic relationship was very close among E. coioides, E. tauvina, and E. malabaricus and also between E. chlorostignia and E. bleekeri. Within-species genetic polymorphism was highest in E. chlorostigma (49.15%) and lowest in E. tauvina (25.42%).


PMID: 15136913

DOI: 10.1007/s10126-003-0021-9

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Schaafsma, A.W.; Baufeld, P.; Ellis, C.R., 1999: Influence of cropping practices on corn rootworm in Canada as a basis for assessment of the potential impact of Diabrotica virgifera in Germany. Monitoring of populations of adult corn rootworms (Diabrotica virgifera and D. barberi) in Ontario (Canada), and surveys of insecticide use against rootworms, showed that populations declined in the last 10 years and that the proportion of the mai...

Weir, J., 1925: Brachiopoda, Lamellibranchiata, Gastropoda and Belemnites in Wyllie, B K N and Smellie, W R On the collection of Fossils and Rocks from Somaliland. Monographs of the Geological Department of the Hunterian Museum Glasgow University I: I 6: 80-85

Botwinick, C.Y.; Quartermain, D.; Freedman, L.S.; Hallock, M.F., 1977: Some characteristics of amnesia induced by FLA-63 an inhibitor of dopamine beta hydroxylase. The amnesic effects of FLA-63, a potent dopamine-beta-hydroxylase (DBH) inhibitor, were investigated in a food motivated spatial discrimination task. Groups of C57BL/6J mice were injected with either 5 mg/kg, 15 mg/kg, 25 mg/kg, 35 mg/kg or physio...

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Rees, A.; Elbl, F.; Solinger, R.; Gattinella, J.; Lambert, L.; Cook, L., 1981: Detecting ductal shunting in premature infants by range-gated Doppler echocardiography. Range-gated pulsed Doppler echocardiography has been reported to be a useful noninvasive bedside technic for detecting ductal left-to-right shunting in premature infants. We studied 30 premature infants with a birth weight of less than 1,500 gm, u...

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