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The use of newly developed real-time PCR for the rapid identification of bacteria in culture-negative osteomyelitis

, : The use of newly developed real-time PCR for the rapid identification of bacteria in culture-negative osteomyelitis. Joint, Bone, Spine 73(6): 745-747

We report a case of a culture-negative osteomyelitis in which our newly developed real-time polymerase chain reaction (PCR) could differentiate Staphylococcus aureus from Staphylococcus epidermidis. This is the first report that described the application of this novel assay to an orthopedics clinical sample. This assay may be useful for other clinical culture-negative cases in a combination with a broad-spectrum assay as a rapid microorganism identification method.


PMID: 16650790

DOI: 10.1016/j.jbspin.2005.11.014

Other references

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Nitanda, A.; Yasunami, N.; Tokumo, K.; Fujii, H.; Hirai, T.; Nishio, H., 2005: Contribution of the peripheral 5-HT 2A receptor to mechanical hyperalgesia in a rat model of neuropathic pain. We investigated the effect of 5-HT receptor antagonists on mechanical hyperalgesia observed in a neuropathic pain rat model prepared by chronic constriction injury of the sciatic nerve. NAN-190, a 5-HT 1A receptor antagonist, (-)-pindolol, a 5-HT...

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Schnell, W., 1954: The suitability of maize hybrids and maize varieties for cultivation (in Western Germany). There has recently been a renewed interest in Western Germany in the cultivation of maize, particularly for fodder. This paper gives the most important results of variety trials, carried out during 1950-2, with seed of known pedigree provided by F...

Thomas-Schwan-Tschöpe, 1998: "Social marketing" is no longer a concept but an awareness. Pflege Aktuell 52(1): 38-39

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Mcgovern, W.L.; Mckibben, G.H.; Cross, W.H.; Essig, H.W.; Lindig, O.H., 1976: Boll weevil square ingestion and utilization studies. Laboratory-reared .male. boll weevils, Anthonomus grandis Boheman, ate .apprx. 13 mg of squares per day though field-collected weevils ate only 10 mg/day; the field weevils were 9% more efficient in the utilization of squares. Laboratory weevils u...

Rapała, K.; Chaberek, Sławomir.; Truszczyńska, A.; Lukawski, Sław.; Walczak, P., 2009: Digital computed tomography affords new measurement possibilities in lumbar stenosis. The problem of lumbar stenosis has been analysed at length in the literature as the number of patients with low back pain of this aetiology continues to increase. The aim of this study was:1. to carry out a prospective study assessing the dimensio...