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Tissue expression of alternatively spliced GFRalpha1, NCAM and RET isoforms and the distinct functional consequence of ligand-induced activation of GFRalpha1 isoforms

, : Tissue expression of alternatively spliced GFRalpha1, NCAM and RET isoforms and the distinct functional consequence of ligand-induced activation of GFRalpha1 isoforms. Brain Research. Molecular Brain Research 139(1): 1-12

Glial-cell-line-derived neurotrophic factor (GDNF) exerts its effect through a multi-component receptor system consisting of GFRalpha1, RET and NCAM. Two highly homologous alternatively spliced GFRalpha1 isoforms (GFRalpha1a and GFRalpha1b) have previously been identified. In this study, isoform specific real-time PCR assays were used to quantify the expression levels of GFRalpha1, RET and NCAM isoforms in murine embryonic and adult tissues. The expression levels of GFRalpha1b were found to be comparable to that of GFRalpha1a in peripheral tissues. However, GFRalpha1a was the predominant isoform expressed in the whole brain. The co-expressions of GFRalpha1 and the co-receptors were developmentally regulated and differentially expressed in some tissues. Microarray analyses of GFRalpha1 isoforms transfected cells stimulated with NTN showed distinct and non-overlapping gene profiles. These observations are consistent with the emerging view that the combinatorial interactions of the spliced isoforms of GFRalpha, RET and NCAM may contribute to the pleiotropic biological responses.


PMID: 15979200

DOI: 10.1016/j.molbrainres.2005.05.016

Other references

Goday, A., 1998: Composition of master formulas for the treatment of obesity. Anales de Medicina Interna 15(5): 287-288

Adlercreutz, H.; Mazur, W.; Bartels, P.; Elomaa, V.; Watanabe, S.; Wähälä, K.; Landström, M.; Lundin, E.; Bergh, A.; Damber, J.E.; Aman, P.; Widmark, A.; Johansson, A.; Zhang, J.X.; Hallmans, G., 2000: Phytoestrogens and prostate disease. Part of a special supplement on the Third International Symposium on the Role of Soy in Preventing and Treating Chronic Disease. Recent studies that indicate that dietary phytoestrogens may have a role as protective compounds with regard to prosta...

Whitaker, J O.; Jr.; Maser, C., 1976: Food habits of fine western Oregon shrews. Northwest Science Cheney, 502: 102-107

Thompson P.I.; Jones T.S.; O'reilly K., 1989: Promotion of hiv prevention outreach activities targeting intravenous drug users in the usa. Morisset, R A (Ed ) Ve Conference Internationale Sur Le Sida: Le Defi Scientifique Et Social; V International Conference on Aids: The Scientific And Social Challenge; Montreal, Quebec, Canada, June 4-9, 1262p International Development Research Centre: Ottawa, Ontario, Canada Illus Paper 849

Kirkegaard, C.; Norlem, N.; Lauridsen, U.B.; Bjorum, N.; Christiansen, C., 1975: Protirelin stimulation test and thyroid function during treatment of depression. Thyroid levels were estimated in 15 patients with endogenous depressions. Before electroconvulsive treatment (ECT), serum thyroxine (T4) and free T4 index values were elevated (P less than .02). After recovery from depression, the levels were norm...

Saran, R.; Gupta, A.K.; Shrivastava, S.N.; Prasad, L.S., 1986: Use of modified Grace's insect medium for the primary isolation of Leishmania donovani donovani in Bihar, India. Grace's insect medium, supplemented with 20% (v/v) heat-inactivated fetal bovine serum (FBS) and 15% defibrinated pooled rabbit blood is statistically shown to be more sensitive than modified NNN medium (P less than 0.05), Grace's insect...

Friedlander, B., 1899: Ueber noch wenig bekannte kosmische Einflusse auf physiologische Vorgange. Verb Berlin Physiol Ges in Arch Physiol, 570-574

Egan, J.; Cutney, C.; Scorza, W.; Cusick, W.; Smulian, J.; Vintzileos, A., 1994: The feasibility of obtaining a five view fetal echocardiogram within ten minutes during a level I or II ultrasound form 16-36 weeks. American Journal of Obstetrics & Gynecology 170(1 PART 2): 367

Beisecker, A.E.; Murden, R.A.; Moore, W.P.; Graham, D.; Nelmig, L., 1996: Attitudes of medical students and primary care physicians regarding input of older and younger patients in medical decisions. This study was designed to determine whether medical students, residents, and fully trained physicians differ in their attitudes toward decision-making input by older and younger patients, whether they believe that physicians should have greater i...

Aksen, F.; Akdag, M.Zulkuf.; Ketani, A.; Yokus, B.; Kaya, A.; Dasdag, S., 2006: Effect of 50-Hz 1-mT magnetic field on the uterus and ovaries of rats (electron microscopy evaluation). The aim of this study was to investigate the effect of extremely low frequency magnetic fields (ELFMF) on the uterus and ovary of rats. Forty-eight female Wistar albino rats were divided into two groups, one for 50 and the other for 100 days of ex...