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Whole-genome analysis to identify type III-secreted effectors


, : Whole-genome analysis to identify type III-secreted effectors. Methods in Molecular Biology 354: 19-34

Many Gram-negative plant and animal pathogens share a common virulence strategy that relies on the specialized type III secretion system. This apparatus is used to secrete virulence factors, called effectors, into the extracellular host environment and directly into the cytoplasm of host cells. Effectors interfere with host signaling and host metabolism to create an optimal environment for pathogen replication. The identification of effectors in plant pathogens was limited for many years to those effectors that elicit strong plant defenses on some hosts. The members of this subset, called avirulence proteins, can be readily identified because they dominantly confer strong defense-inducing properties to a heterologous virulent strain. This chapter describes two methods to identify type III-secreted effectors in plant pathogens independently of their phenotype. The first method consists of an in vivo molecular genetic screen that uses the activity of an avirulence protein to identify effectors without avirulence activity. It should be possible to apply this method to most Gram-negative plant pathogens. The second method consists of a bioinformatic approach applicable to those pathogens for which at least a draft genome sequence is available.

US$19.90

PMID: 17172741

DOI: 10.1385/1-59259-966-4:19


Other references

Axlund, T.W.; Hudson, J.A., 2003: Computed tomography of the normal lumbosacral intervertebral disc in 22 dogs. Computed tomography of the lumbosacral spine was performed on 22 purpose-bred, medium-sized hound dogs between 1 and 6 years of age. Images were evaluated to determine the degree of disc bulge. All dogs were neurologically normal. Dogs were imaged...

Sprague, R.G., 1953: Cushing's syndrome with special reference to bilateral adrenalectomy. Proceedings of the Royal Society of Medicine 46(12): 1070-1077

Cordeiro, T.N.; Schmidt, H.; Madrid, C.; Juárez, A.; Bernadó, P.; Griesinger, C.; García, Jús.; Pons, M., 2012: Indirect DNA readout by an H-NS related protein: structure of the DNA complex of the C-terminal domain of Ler. Ler, a member of the H-NS protein family, is the master regulator of the LEE pathogenicity island in virulent Escherichia coli strains. Here, we determined the structure of a complex between the DNA-binding domain of Ler (CT-Ler) and a 15-mer DNA...

Romaniuk, K.; Tarczynski, S., 1976: Ranide-MSD and ranizole-MSD in treatment of fascioliasis and gastro-intestinal nematodiases of sheep. Sheep naturally infected with Fasciola hepatica and gastro-intestinal nematodes were given orally before feeding 0.33 ml/kg body-weight of either Ranide (rafoxanide) or Ranizole (rafoxanide plus thiabendazole) and the results assessed on faecal ex...

Ng, E.Shu-Ting.; Vijayan, J.; Therimadasamy, A.; Tan, T.Chyan.; Chan, Y.Cheun.; Lim, A.; Wilder-Smith, E., 2010: The added value of preoperative ultrasonography of the ulnar nerve: an observational study. Muscle & Nerve 42(4): 613-614

Chand T., 1987: Desorption of copper as studied by different extractants. Desorption of copper by different extracting reagents was studied to evaluate their extracting ability in sierozem soils of Haryana (India). The extracting power of the different extractant was in the order: 0.02 M EDTA > citrate-EDTA > 0.1N...

Moreadith, R.W.; Lehninger, A.L., 1984: The pathways of glutamate and glutamine oxidation by tumor cell mitochondria. Role of mitochondrial NAD(P)+-dependent malic enzyme. Little evidence has been available on the oxidative pathways of glutamine and glutamate, the major respiratory substrates of cancer cells. Glutamate formed from glutamine by phosphate-dependent glutaminase undergoes quantitative transamination by...

Noikura Y.; Baba Y.; Chuman Y., 1975: Relationship between hyper uricemia and glucose tolerance. Igaku Kenkyu 45(1): (rECD

Sherwood, P.R.; Given, B.A.; Given, C.W.; Schiffman, R.F.; Murman, D.L.; Lovely, M.; von Eye, A.; Rogers, L.R.; Remer, S., 2006: Predictors of distress in caregivers of persons with a primary malignant brain tumor. The purpose of this cross-sectional, descriptive study was to identify predictors of distress for family caregivers of persons with a primary malignant brain tumor (PMBT). The effect of the care recipient's functional, cognitive, and neuropsy...

Yamagishi, H.; Yoshizako, F.; Sato, K., 1966: Characteristics of DNA molecules extracted from bacteriophages 80 and 80pt. [phi] 80 DNA prepared by a mild phenol extraction method had a buoyant density in a CsCl gradient of 1.710 g cm-3 and a thermal denaturation temperature of 90.5[degree]. The buoyant density and thermal denaturation temperature of [phi] 80pt1 DNA w...