+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

14-3-3sigma controls corneal epithelial cell proliferation and differentiation through the Notch signaling pathway

, : 14-3-3sigma controls corneal epithelial cell proliferation and differentiation through the Notch signaling pathway. Biochemical and Biophysical Research Communications 392(4): 593-598

14-3-3sigma (also called stratifin) is specifically expressed in the stratified squamous epithelium and its function was recently shown to be linked to epidermal stratification and differentiation in the skin. In this study, we investigated its role in corneal epithelium cell proliferation and differentiation. We showed that the 14-3-3sigma mutation in repeated epilation (Er) mutant mice results in a dominant negative truncated protein. Primary corneal epithelial cells expressing the dominant negative protein failed to undergo high calcium-induced cell cycle arrest and differentiation. We further demonstrated that blocking endogenous 14-3-3sigma activity in corneal epithelial cells by overexpressing dominative negative 14-3-3sigma led to reduced Notch activity and Notch1/2 transcription. Significantly, expression of the active Notch intracellular domain overcame the block in epithelial cell differentiation in 14-3-3sigma mutant-expressing corneal epithelial cells. We conclude that 14-3-3sigma is critical for regulating corneal epithelial proliferation and differentiation by regulating Notch signaling activity.


PMID: 20100467

DOI: 10.1016/j.bbrc.2010.01.084

Other references

Klusacek, J.; Kvapil, O., 1968: Comparison of fattening ability and carcass characters of Czechoslovak Improved White and Landrace pigs, based on evaluation of genealogical lines. Records were compared of 1304 progeny groups of 498 boars from 69 Czechoslovak Improved White (C.I.W.) lines and of 224 progeny groups by 72 boars from 23 Landrace lines. In C.I.W. and Landrace pigs resp., number of days from birth to 90 kg live w...

Kim, M.; Salley, S.O.; Ng, K.Y.S., 2008: Transesterification of Glycerides Using a Heterogeneous Resin Catalyst Combined with a Homogeneous Catalyst. Energy & Fuels 22(6): 3594-3599

Martin, L.; Hagar, S., 1990: Bird control on containment pond sites. Proceedings Vertebrate Pest Conference: (14th) 307-310

Knol, E.F.; Kuijpers, T.W.; Mul, F.P.; Roos, D., 1993: Stimulation of human basophils results in homotypic aggregation. A response independent of degranulation. For a better insight into the mechanisms determining the recruitment of human basophilic granulocytes from the circulation to sites of allergic reactions, we studied the homotypic aggregation of these cells. The aggregation was studied with > 9...

Robertson, L.S.; Haddon, W., 1974: The buzzer-light reminder system and safety belt use. American Journal of Public Health 64(8): 814-815

Matos, D.Leite.; Lima-Costa, M.Fernanda.; Guerra, H.L.; Marcenes, W., 2002: Bambuí Project: an evaluation of private, public and unionized dental services. Since one of the goals of dental services is to reduce the effects of social inequality on dental health, it was assessed whether there are inequalities among those seeking dental care from private, public and unionized services. The study populat...

Shrestha, M.K.; Lalchan, S., 2011: Adrenal gland teratoma in a 40-year-old woman. Teratoma is a germ-cell tumor that commonly affects the gonads. Extragondal teratoma is a rare entity. Teratoma in the region of adrenal gland is a rare and uncommon retroperitoneal tumor; only few cases have been reported. This case report descri...

Phillips, R.J.; Malin, M.C., 1980: Ganymede: a relationship between thermal history and crater statistics. An approach for factoring the effects of a planetary thermal history into a predicted set of crater statistics for an icy satellite is developed and forms the basis for subsequent data inversion studies. The key parameter is a thermal evolution-de...

Harashina, T.; Wakamatsu, K.; Kitazawa, T., 1996: How to harvest a septal chondromucosal graft. A simple technique to facilitate easier harvesting of a septal chondromucosal graft is presented. Turning over the ala (like a swinging door) using a perialar incision greatly helps easy harvesting of the graft under extremely good direct vision.

Girard, G.Illiat, S.Mon, 1953: Isonicotinic acid hydrazide in therapeutics. Revue Medicale de Nancy 78: 898-904