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5-lipoxygenase and cyclooxygenase-2 in porcine parasitic bronchopneumonia: immunohistochemical and biochemical investigations


, : 5-lipoxygenase and cyclooxygenase-2 in porcine parasitic bronchopneumonia: immunohistochemical and biochemical investigations. Journal of Comparative Pathology 142(2-3): 139-146

Eicosanoids are products of arachidonic acid metabolism and have numerous biological roles. The present study aimed to investigate the role of 5-lipoxygenase (5-LOX)- and cyclooxygenase-2 (COX-2)- dependent enzymatic pathways in the pathogenesis of porcine parasitic bronchopneumonia caused by Metastrongylus spp. Pulmonary tissue samples from healthy control and parasitized pigs were processed for histopathological, immunohistochemical and biochemical investigations. In control animals, immunohistochemistry demonstrated that 5-LOX and COX-2 expression was almost exclusively limited to the bronchiolar epithelial cells. Parasitized pigs had greater 5-LOX- and COX-2- specific immunoreactivity, involving a wide range of cell types within foci of granulomatous and eosinophilic bronchopneumonia. Biochemical investigations demonstrated the presence of 5-LOX (and the related product Leukotriene B(4)) and COX-2 (and the related product prostaglandin E(2); PGE(2)) in all tissues under study. COX-2 activity and PGE(2) concentration were significantly higher in diseased lungs compared with normal healthy controls. These findings demonstrate that 5-LOX and COX-2 are differentially expressed in normal versus lungworm-infected lungs and therefore suggest that both biochemical pathways are likely to be involved in the pathogenesis of porcine parasitic bronchopneumonia.

US$19.90

PMID: 19906385

DOI: 10.1016/j.jcpa.2009.09.004


Other references

Rhee, C.M.; Unruh, M.; Chen, J.; Kovesdy, C.P.; Zager, P.; Kalantar-Zadeh, K., 2014: Infrequent dialysis: a new paradigm for hemodialysis initiation. Nearly a half-century ago, the thrice-weekly hemodialysis schedule was empirically established as a means to provide an adequate dialysis dose while also treating the greatest number of end-stage renal disease (ESRD) patients using limited resourc...

Pearson, W.H.; Elston, R.A.; Bienert, R.W.; Drum, A.S.; Antrim, L.D., 1999: Why did the Prince William Sound, Alaska, Pacific herring fisheries collapse in 1993 and 1994? Review of hypotheses. Following record harvests of Pacific herring (Clupea pallasi) in Prince William Sound, Alaska, in the 3 years after the Exxon Valdez oil spill, the fishery failed in 1993. The hypotheses advanced to explain this dramatic 1993 decline occur in thre...

Iordanov, B.I., 1962: A case of pre-exanthematous varicellous encephalomyelitis (encephalitis pre-exanthemosa varicellosa). Suvremenna Meditsina 13(4): 43-45

Burton, R.I.; Eaton, R.G., 1973: Common hand injuries in the athlete. Orthopedic Clinics of North America 4(3): 809-838

Schouman M.; Amer M.; Ntshaykolo B.; Constantinides C., 1990: Evaluation and management of masculine impotence. Semaine des Hopitaux 66(5): 230-234

Fraga, C.N. de, 2004: Bulbophyllum boudetiana (Orchidaceae), a new species from the Brazilian Atlantic forest, Espirito Santo. Bulbophyllum boudetiana sp. nov., a new species from Espirito Santo, Brazil, that grows in saxicolous vegetation mats on an inselberg in the Brazilian Atlantic forest, is described, illustrated, and compared with related species. This new species...

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Beiderbeck, Rolf., 2005: Dual aseptic culture of gall-inducing arthropods and their host plants. To increase our knowledge on gall etiology and the regulation of gene expression and subordinate processes in gall-building tissues of plants, methods to generate galls throughout the year are indispensable. One promising method is dual aseptic cu...

DasGupta, R.; Fuchs, E., 1999: Multiple roles for activated LEF/TCF transcription complexes during hair follicle development and differentiation. LEF/TCF DNA-binding proteins act in concert with activated beta -catenin, the product of Wnt signaling, to transactivate downstream target genes. To probe the role of activated LEF/TCF transcription factor complexes in hair follicle morphogenesis...

Ascone, L.; Jaya, E.S.; Lincoln, T.M., 2016: The effect of unfavourable and favourable social comparisons on paranoid ideation: An experimental study. Low social rank is associated with paranoia, but there is a lack of evidence for causality. We tested the effects of social comparisons on negative affect and paranoia with an online social rank paradigm, and whether striving to avoid inferiority...