geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

A new safe, simple and successful vitrification method for bovine and human blastocysts


, : A new safe, simple and successful vitrification method for bovine and human blastocysts. Reproductive Biomedicine Online 17(3): 360-367

This study examined a new method for vitrification of blastocysts that is safe, simple and easy to learn and use. Current vitrification techniques have shortcomings that include the use of dimethyl sulphoxide, one of the more toxic cryoprotectants, and minute containers that are difficult to handle and are usually open to contamination. Cell handling and loading times are very short, which allows no room for user-associated errors and increases the difficulty of the procedure. This study describes a method of vitrification without these shortcomings. Human and bovine blastocysts were exposed to a series of three cryoprotectant solutions and loaded into a 0.25 ml sterile straw, heat sealed at both ends and vitrified. This technique allowed sufficient time for cryoprotectant exposure, loading, sealing and vitrification. Research blastocysts were thawed, cultured for 24 h, and stained for cell viability. The majority survived and on average had few lysed cells. In clinical studies from three different centres, 81.4% of vitrified blastocysts were intact after thawing. Out of 43 transfers with 76 blastocysts replaced, 44.7% implanted, 43.4% yielded a fetal heart beat, and a total of 32 babies have been delivered or are ongoing. The overall clinical pregnancy per transfer rate was 60.4%. The high survival rates and clinical pregnancy rates obtained with this new, safe and easy-to-use vitrification procedure are encouraging.

US$29.90

PMID: 18765006


Other references

Hayden, K.M.; Zandi, P.P.; Lyketsos, C.G.; Tschanz, J.T.; Norton, M.C.; Khachaturian, A.S.; Pieper, C.F.; Welsh-Bohmer, K.A.; Breitner, J.C.S., 2005: Apolipoprotein E genotype and mortality: findings from the Cache County Study. OBJECTIVES: To evaluate the association between apolipoprotein E (apo E) epsilon 4 and mortality, the population attributable risk for mortality with epsilon 4, and relative contributions of cardiovascular disease (CVD) and Alzheimer's diseas...

Fass J.; Klose K.C.; Raguse T., 1986: Computed tomography as an aid to differential diagnosis and determination of indication for surgery in inflammatory bowel disease. Langenbecks Archiv fuer Chirurgie 369: 802

Buda, R.; Pagliazzi, G.; Castagnini, F.; Cavallo, M.; Giannini, S., 2016: Treatment of Osteochondritis Dissecans of the Talus in Skeletally Immature Population: A Critical Analysis of the Available Evidence. Juvenile osteochondritis dissecans of the talus (JOCDT) is a subchondral bone alteration and a partially or completely detached osteochondral fragment, affecting skeletally immature patients. A review of the English literature on PubMed was conduc...

Yang YanYan; Zhang GaiPing; Deng RuiGuang; Liu LiYan; Howard, C.J.; Sop, P., 1995: Preparation and identification of monoclonal antibodies to bovine IgG Fc receptors. The methods for preparation and identification of monoclonal antibodies to cell surface molecules were established using positive expressing cells and fluorescence activated flow cytometry. COS7 cells were transfected with bovine IgG1Fc receptor (...

Yoshihara, S.; Suzuki, F.; Fujita, H.; Geng, X.X.; Ikeuchi, M., 2001: Novel putative photoreceptor and regulatory genes Required for the positive phototactic movement of the unicellular motile cyanobacterium Synechocystis sp. PCC 6803. Synechocystis: sp. PCC 6803 is a unicellular motile cyanobacterium, which shows positive or negative phototaxis on agar plates under lateral illumination. By gene disruption in a substrain showing of positive phototaxis, it was demonstrated that m...

Ryadchikov, V.G.; Zakharova, N.V.; Uletova, N.P., 1981: Silage from low-lignin maize for feeding young bulls. Silage prepared from a five-line hybrid, (BC3Sg25 X BC2G23) X [(A554 X W153R) X W117], and its counterpart with bm3bm3 for brown midrib was tested using Krasnodar 334 as a control. Weight gain in feeding tests was 15.9% greater with the bm3 silage...

Louizidou, P.; Thessalou-Legaki, M.; Zenetos, A., 2007: First record of the Red Sea immigrant Chrysallida fischeri (Hornung and Mermod, 1925) (Gastropoda: Pyramidellidae) from Greek waters. The Red Sea mollusc Chrysallida fischeri (Hornung and Mermod, 1925) was collected in 2005 off Makri, Rhodes, Greece. This is the first record of the species in the Aegean Sea. The presence of both live specimens and shells suggest that C. fischeri...

Panchenko I.V., 1994: Paleogeodynamic origin of the ophiolitic complexes on the Arctic margin of the Russian Far East. Pages 88-89 1994

Munari, M.; Zucchetta, P.; Carollo, C.; Gallo, F.; D.N.rdin, M.; Marzola, M.Cristina.; Ferretti, S.; Facco, E., 2005: Confirmatory tests in the diagnosis of brain death: comparison between SPECT and contrast angiography. Cerebral blood flow tests have increasingly been advocated for the confirmation of brain death (BD). Four-vessel angiography has been considered the most reliable investigation in the diagnosis of BD for >30 yrs, but it is invasive. (99m)Tc-HMP...

Blagosklonov, K.N., 1968: The nesting trees of the woodpeckers in the various types of the forest. Ornitologiya, 9: 95-102