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A recombinant West Nile virus envelope protein vaccine candidate produced in Spodoptera frugiperda expresSF+ cells


, : A recombinant West Nile virus envelope protein vaccine candidate produced in Spodoptera frugiperda expresSF+ cells. Vaccine 27(2): 213-222

In this study, a recombinant truncated West Nile virus envelope protein antigen (rWNV-E) was produced in serum-free cultures of the expresSF+ insect cell line via baculovirus infection. This production system was selected based on its use in the production of candidate human and animal vaccine antigens. A defined fermentation and purification process for the rWNV-E antigen was established to control for purity and immunogenicity of each protein batch. The material formulated with aluminum hydroxide was stable for greater than 8months at 4 degrees C. The recombinant vaccine candidate was evaluated for immunogenicity and protective efficacy in several animal models. In mouse and hamster WNV challenge models, the vaccine candidate induced viral protection that correlated with anti-rWNV-E immunogenicity and WNV neutralizing antibody titers. The rWNV-E vaccine candidate was used to boost horses previously immunized with the Fort Dodge inactivated WNV vaccine and also to induce WNV neutralizing titers in naïve foals that were at least 14weeks of age. Furthermore, the vaccine candidate was found safe when high doses were injected into rats, with no detectable treatment-related clinical adverse effects. These observations demonstrate that baculovirus-produced rWNV-E can be formulated with aluminum hydroxide to produce a stable and safe vaccine which induces humoral immunity that can protect against WNV infection.

US$19.90

PMID: 18996430

DOI: 10.1016/j.vaccine.2008.10.046


Other references

Fritz, J.S.; Prazee, R.T.; Latwesen, G.L., 1970: Column chromatographic separation of gallium, indium and thallium. Distribution ratios are given for the extraction of gallium-(III), indium(III) and thallium(III) from aqueous solutions of hydrobromic acid into di-isopropy ether and isobutyl methyl ketone. Based on the results obtained, a scheme is presented for...

Ibrahima Sow, Robert K.Dixon, Jie Pan…, 2014: Financing for innovative technologies and best practices to reduce persistent organic pollutants. Persistent Organic Pollutants (POPs) threaten human health and the global environment. Recognizing their dangers many countries began to limit or ban POPs production, use, and release in the 1990s. Eventually the Stockholm Convention on POPs, was...

Tan, Y-xia.; Chen, T.; Zhong, S-qing.; Li, Y-min.; Chen, Y-yu.; Qiu, X-yan.; Mo, X-ning.; Song, Q-sheng.; Gan, Q-ni.; Gu, Y-ying.; Mo, H-ying.; Luo, W.; He, X-ming.; Liu, M-chang.; Zhong, N-shan., 2006: Expression and significance of severe acute respiratory syndrome associated coronavirus (SARS-CoV)-X4 protein in lungs of SARS patients. To investigate the significance of severe acute respiratory syndrome associated coronavirus (SARS-CoV)-X4 protein expression in lungs of patients with SARS. Pathological features of the lungs from 4 SARS patients were examined and the expression o...

Philippe L.; Dubos B.; L.M.nn R., 1987: Cytochemical study of the action of stereum hirsutum on the walls of vine wood. Biology of the Cell (Paris) 60(1): 15A

Zhang, X.F.; Luo, T.Y., 2016: Association between the FGB gene polymorphism and ischemic stroke: a meta-analysis. To clarify the relationship between the β-fibrinogen (FGB) genetic polymorphism (-148C>T) and ischemic stroke, we identified studies by searching PubMed, EMBASE, and the Chinese National Knowledge Infrastructure (CKNI) databases. Data from eli...

Vereshchagin N.K., 1977: The Berelekh mammoth cemetery. Trudy Zoologicheskogo Instituta 72: 5-50

Schwartz, D.A.; Loftus, E.V.Jr; Tremaine, W.J.; Panaccione, R.; Harmsen, W.S.ott; Zinsmeister, A.R.; Sandborn, W.J., 2002: The natural history of fistulizing Crohn's disease in Olmstead County, Minnesota. Background and Aims: Little is known about the cumulative incidence and natural history of fistulas in Crohn's disease in the community. Methods: The medical records of all Olmsted County, Minnesota residents who were diagnosed with Crohn&apo...

Valeri C.R.; Valeri D.A.; Gray A.; Melaragno A.; Dennis R.C.; Emerson C.P., 1982: Red blood cell concentrates stored at 4 celsius for 35 days in citrate phosphate dextrose adenine 1 citrate phosphate dextrose adenine 2 or citrate phosphate dextrose adenine 3 anti coagulant preservative biochemically modified and frozen and stored in the poly vinyl chloride plastic primary collection bag with 40 percent weight volume glycerol at minus 80 celsius. Human red blood cell concentrates drawn from normal healthy volunteers with hematocrits of 85 .+-. 5 V % were stored in the CPDA-1 [citrate, phosphate, dextrose, adenine], CPDA-2 or CPDA-3 anticoagulant at 4.degree. C for 35 days. After liquid sto...

Busque, J.; Fernandez, N.; Fernandez, B., 2006: A decision support tool to design rangeland sustainable grazing systems. Mountain rangelands of northern Spain are valuable ecological and economical communal resources. Cattle and horse summer grazing have historically shaped these landscapes and continue to be their main revenue. In the last decades temporal and spat...

Jayaraman S.; Sheth A.R., 1980: Contraceptive efficacy of testosterone estradiol implant in male rats. The contraceptive efficacy of simultaneous administration of testosterone and estradiol through subdermal silastic implants was investigated in adult male rats. All the males showed complete sterility after 7 wk of treatment, which persisted till...