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A role of functional T-type Ca2+ channel in hepatocellular carcinoma cell proliferation


, : A role of functional T-type Ca2+ channel in hepatocellular carcinoma cell proliferation. Oncology Reports 22(5): 1229-1235

The role of T-type Ca2+ channels in hepatocellular carcinoma cell proliferation was investigated in vitro. Eleven hepatocellular carcinoma cell lines and one immortalized liver cell line (LO2) were examined for the status of T-type Ca2+ channels with RT-PCR and voltage-clamp recordings. Except HBxF344, other cell lines tested had one, two or all three of alpha1-subunits (alpha1G, alpha1H and alpha1I) mRNA expression. Obvious T-type current was recorded in SNU449 cells, while others exhibited a minimal or no T-type current. SNU449, PLC/PRF5, Hep3B and LO2 cell lines were subjected to growth assay in the presence of Mibefradil, a T-type Ca2+ channel blocker, only the proliferation of SNU449 cell which had functional T-type Ca2+ channel was reduced by Mibefradil treatment. Furthermore, the persistent increase of phosphorylated ERK1/2 in SNU449 cells was found when treated with Mibefradil. A microarray assay also demonstrated some down-regulated genes were mainly associated with cell cycle and cell proliferation in Mibefradil treated SNU449 cells. In conclusion, this study showed that the functional T-type Ca2+ channels probably participate in modulating the proliferation of some hepatocellular carcinoma cells. The cell proliferation reduction of SNU449 with Mibefradil treatment is possibly associated with the persistent increase of phosphorylated ERK1/2.

US$29.90

PMID: 19787244


Other references

Buchan, J.C.; Gilbert, C., 2003: Ocular and financial health. British Journal of Ophthalmology 87(4): 518-518

Schraven, S.P.; Bisdas, S.; Wagner, W., 2013: Synchronous spontaneous cerebrospinal fluid leaks in the nose and ear. The majority of spontaneous cerebrospinal fluid leaks occur at the anterior skull base; few cases at the temporal bone have been described. There have been no previous reports of synchronous leaks at the anterior skull base and temporal bone in th...

Mcquaid, C.D.; Froneman, P.W., 2004: The Southern Ocean Group at Rhodes University: seventeen years of biological oceanography in the Southern Ocean reviewed. This paper reviews the main findings of the Southern Ocean Group at Rhodes University over the last 17 years. A primary contribution has been the development of conceptual models of the physicalbiological driving mechanisms that support enormous s...

Liyana Pathirana, C.; Shahidi, F.; Whittick, A., 2002: Comparison of nutrient composition of gonads and coelomic fluid of green sea urchin Strongylocentrotus droebachiensis. The compositional characteristics of sea urchin gonads and coelomic fluid from Strongylocentrotus droebachiensis harvested in the coasts of Newfoundland and thereafter reared in an aquaculture facility and fed on a Laminaria diet for a 3-week peri...

Rosatte, R.C.; Power, M.J.; MacInnes, C.D., 1991: Ecology of urban skunks, raccoons, and foxes in metropolitan Toronto. Unknown

Bhatia, J.; Rossignol, C.; Falls, D.G.eer; Rassin, D.K., 1994: Expression of glutamate dehydrogenase in parenterally-fed neonatal piglets. Pediatric Research 37(4 PART 2): 303A

Lang, E.K., 1964: Meatal Stenosis With Resulting Ureteral Reflux. Journal of the Indiana State Medical Association 57: 765-766

Galicia, M.A.; Pelegri, A.; Rodriquez, J.A.; Olmos, A.; Segarra, A.; Piera, L., 1993: Therapy of secondary hyperparathyrodism with intermittent oral bolus of 1,25 dihydroxyvitamin D3. Bone (New York) 14(1): 76

van den Nieuwenhuizen, Arend., 2002: A tropical house in the living-room Een tropenhuis in de huiskamer slot. Aquariumwereld. December; 5512: 286-290

Schreiber, B., 1931: Due casi di anomalia negli ossicini di Weber. Atti Soc Milano: 228-239