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A transmembrane domain-containing surface protein from Toxoplasma gondii augments replication in activated immune cells and establishment of a chronic infection


, : A transmembrane domain-containing surface protein from Toxoplasma gondii augments replication in activated immune cells and establishment of a chronic infection. Infection and Immunity 77(9): 3731-3739

Toxoplasma gondii mutants identified as defective in the establishment of chronic infection were screened to isolate those specifically impaired in their ability to replicate within activated macrophages. One of the identified mutants contains an insertion in the hypothetical gene TGME49_111670. Genetic complementation restores the ability of the mutant to replicate in immune cells and produce cysts in the brains of mice. While the mutant is more sensitive to nitric oxide than is its parental strain, it is not defective in its ability to suppress nitric oxide. The disrupted protein has no significant homology to proteins with known functions, but is predicted to have one transmembrane domain. Immunofluorescence shows the protein on the parasite surface, even in activated macrophages, colocalizing with a tachyzoite surface antigen, SAG1, and oriented with its C-terminal end external. Western analysis reveals that the protein is downregulated in bradyzoites. Despite the tachyzoite specificity of this protein, mice infected with the mutant succumb to acute infection similarly to those infected with the parent strain. Serum samples from mice with chronic T. gondii infection react to a polypeptide from TGME49_11670, indicating that the protein is seen by the immune system during infection. This study is the first to characterize a T. gondii surface protein that contains a transmembrane domain and show that the protein contributes to parasite replication in activated immune cells and the establishment of chronic infection.

US$19.90

PMID: 19581395

DOI: 10.1128/IAI.00450-09


Other references

Stein, J.H.; Carlsson, C.M.; Papcke Benson, K.; Aeschlimann, S.E.; Carnes, M.L.; Mcbride, P.E., 2001: Aggressive treatment of endothelial dysfunction in geriatric patients The simvastatin, ascorbate, and vitamin E in seniors study. Journal of the American College of Cardiology 37(2 Supplement A): 193A-194A, February

Gow, J.G.; Ross, J.C.; S.H.Ll.C.A., 1959: Partial nephrectomy: a critical study. Lancet 2(7106): 759-761

Castelino, P.Anil.; Naik, P.; Dasappa, J.Prasad.; Sujayraj, R.S.; Sharath Chandra, K.; Chaluvaiah, K.; Nair, R.; Sandya Kumari, M.V.; Kalthur, G.; Adiga, S.Kumar., 2015: Synthesis of novel thiadiazolotriazin-4-ones and study of their mosquito-larvicidal and antibacterial properties. A series of novel 3-tert-butyl-7-(aryloxymethyl)-4H-[1,3,4]thiadiazolo[2,3-c][1,2,4]triazin-4-ones (5a-5n) were synthesized by refluxing 3,3-dimethyl-2-oxobutanoic acid (trimethyl pyruvic acid) (1) and thiocarbohydrazide (2) in ethanol as solvent...

Whitby, C.P.; Fischer, F.E.; Fornasiero, D.; Ralston, J., 2011: Shear-induced coalescence of oil-in-water Pickering emulsions. This work reports on coalescence in oil-in-water Pickering emulsions subjected to simple shear flow. The emulsions were stabilized by silanized fumed silica particles forming layers a few hundred nanometers thick around drops that are tens of micr...

Husted, Søren.; Mattsson, M.; Möllers, C.; Wallbraun, M.; Schjoerring, J.K., 2002: Photorespiratory NH(4)(+) production in leaves of wild-type and glutamine synthetase 2 antisense oilseed rape. Exposure of oilseed rape (Brassica napus) plants to increasing leaf temperatures between 15 degrees C and 25 degrees C increased photorespiratory NH(4)(+) production from 0.7 to 3.5 micromol m(-2) s(-1). Despite the 5-fold increase in the rate of...

Artaud, P.; Bernard, R.; Desanti, F.; Montagnier, J., 1957: Recurrent chondroma in a patient of exostosis. Pediatrie 12(1): 84-84

Saavedra, R.; Pinto, J., 1973: Results in the treatment of cervix uteri carcinoma. Revista Chilena de Obstetricia Y Ginecologia 38(3): 121-131

Bhargava, K.; Kler, T.S.; Wellens, H.J., 2008: An example of combined sinoatrial and atrioventricular block. Combined sinoatrial and atrioventricular block is rare and has been reported in patients on digitalis. We report a case of combined Mobitz type II sinoatrial block and 2:1 atrioventricular block in a patient on no medication who presented with rec...

Martinasso, B.; Lombardi, G., 2004: Remote sensing in NW Italian Alps for pastoral inventory and improvement of grassland management. Thematic maps are helpful tools in the ecologic and agronomic management of native pastures for the possibility of representing land from several different point of view. Drawing territorial maps may take advantage from the use of satellite images...

Maull, K.I.; Yarbrough, B., 1983: Experience with a multidisciplinary emergency medicine clerkship. In 1979 a required emergency medicine clerkship was added to the senior curriculum at the Medical College of Virginia. Coordinated by the Department of Surgery but receiving active support from other disciplines, the emergency medicine clerkship c...