geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Abiotic stress response in yeast and metal-binding ability of a type 2 metallothionein-like protein (PutMT2) from Puccinellia tenuiflora


, : Abiotic stress response in yeast and metal-binding ability of a type 2 metallothionein-like protein (PutMT2) from Puccinellia tenuiflora. Molecular Biology Reports 41(9): 5839-5849

Metallothioneins are low-molecular weight and cysteine-rich metal-binding proteins that play predominant cellular roles in the scavenging of reactive oxygen species and in mediating metal metabolism. To evaluate the role of a type-2 metallothionein-like gene from Puccinellia tenuiflora (PutMT2), the gene was over-expressed in yeast, and growth was assessed under a variety of abiotic stress conditions including peroxide (H2O2), salinity (NaCl and NaHCO3), and metal stress. PutMT2 overexpression in yeast improved the tolerance of cells to H2O2, NaCl, NaHCO3, Zn(2+), Fe(2+), Fe(3+), Cd(2+), Cr(6+), and Ag(+), but increased the sensitivity of cells to Mn(2+), Co(2+), Cu(2+), and Ni(2+) compared with control cells. PutMT2 was then expressed in Escherichia coli BL21as a glutathione S-transferase (GST) fusion protein (GST-PutMT2), and the metal-binding ability of GST-PutMT2 was analyzed and compared with GST alone using inductively coupled plasma atomic emission spectroscopy. Results showed that PutMT2 could bind to Cr, Cd, Co, Ag, Ba, Pb, Mn, Zn, Fe, Cu, P, Al, and Mg, but not Ni and As. There was no evidence to suggest that PutMT2 exhibited a specific or selective binding tendency to any individual metal ion. PutMT2 protein bound to Zn, Na, and Cu in vivo, perhaps with the highest affinity for Cu. Taken together, our results suggest that PutMT2 protein could play an important role in improving metal tolerance by metal binding in yeast. However, additional studies are required to confirm these results and to clarify the function of PutMT2 in transgenic plants.

US$19.90

PMID: 24973879

DOI: 10.1007/s11033-014-3458-1


Other references

Zdravev, P., 1950: Contribution to the study of treatment of otogenous abscesses of the cerebellum. Les Annales D'oto-Laryngologie 67(11-12): 782-796

Shimada, N.; Kawamoto, H.; Saka, S., 2007: Solid-state hydrolysis of cellulose and methyl alpha- and beta-D-glucopyranosides in presence of magnesium chloride. Solid-state hydrolysis proceeded with cellulose and methyl alpha- and beta-D-glucopyranosides in the presence of hydrated magnesium chloride. This reaction was effective even at >100 degrees C since the hydrated water, which is held by MgCl(2)...

Levy, M.M.; Baylor, M.S.; Bernard, G.R.; Fowler, R.; Franks, T.J.; Hayden, F.G.; Helfand, R.; Lapinsky, S.E.; Martin, T.R.; Niederman, M.S.; Rubenfeld, G.D.; Slutsky, A.S.; Stewart, T.E.; Styrt, B.A.; Thompson, B.Taylor.; Harabin, A.L., 2004: Clinical issues and research in respiratory failure from severe acute respiratory syndrome. The National Heart, Lung, and Blood Institute, along with the Centers for Disease Control and Prevention and the National Institute of Allergy and Infectious Diseases, convened a panel to develop recommendations for treatment, prevention, and rese...

Tian, D.H.; Wan, B.; Bannon, P.G.; Misfeld, M.; LeMaire, S.A.; Kazui, T.; Kouchoukos, N.T.; Elefteriades, J.A.; Bavaria, J.; Coselli, J.S.; Griepp, R.B.; Mohr, F.W.; Oo, A.; Svensson, L.G.; Hughes, G.Chad.; Yan, T.D., 2013: A meta-analysis of deep hypothermic circulatory arrest versus moderate hypothermic circulatory arrest with selective antegrade cerebral perfusion. A recent concern of deep hypothermic circulatory arrest (DHCA) in aortic arch surgery has been its potential association with increased risk of coagulopathy, elevated inflammatory response and endorgan dysfunction. Recently, moderate hypothermic c...

Childs, P.A.; Penforld, J.C., 1986: Criteria audit of surgery for carcinoma of large bowel. A comparison between Melbourne teaching hospitals and Royal Perth Hospital. Australian Clinical Review 6(20): 34-37

Smith, J., 2014: A home-birthed neonate in cardiogenic shock. Journal of Emergency Nursing: Jen 39(4): 353-354

Rosler, Hans-Jurgen., 1991: Rachow's notho, the jewel from East Africa. Tropical Fish Hobbyist. May; 399: 30-36

Karanikas, E.P., 2011: Psycho-immunological mechanisms in schizophrenia. Immune alterations in schizophrenia have been described for decades. Both the unspecific and specific arms of the immune system seem to be involved. Several lines of evidence support that an infectious and/or autoimmune process might play a role i...

Nakatani, N.; Fukuda, H.; Fuwa, H., 1979: Major anthocyanin of Bolivian purple corn (Zea mays L.). The main pigment of grain and ears of Bolivian purple maize was identified as cyanidin 3- beta -glucoside.

Klingelfuss, L.H.; Yorinori, J.T., 2000: Latent infection by Colletotrichum truncatum and Cercospora kikuchii and effect of fungicides on late season diseases of soybean. The residual effect and efficacy of fungicides (benomyl, carbendazim, tebuconazole, difenoconazole and acibenzolar-S-methyl) applied to control late season diseases (caused by Septoria glycines and Cercospora kikuchii) of soyabeans were investigat...