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Absolute quantification and modulation of cytochrome P450 3A isoforms in cattle liver

, : Absolute quantification and modulation of cytochrome P450 3A isoforms in cattle liver. Veterinary Journal 202(1): 106-111

In humans and laboratory animals, knowledge about cytochrome P450 (CYP) regulation and function is detailed and very extensive. However, CYPs have still been incompletely characterized in veterinary species so far. In this study, mRNA levels of three CYP3A enzymes (CYP3A28, CYP3A38 and CYP3A48) were measured in cattle liver by using quantitative real-time RT-PCR (qPCR) assays and an absolute quantification approach. In particular, the possible presence of breed-differences in CYP3A expression was investigated in five different meat cattle breeds (Charolais, CH; Piedmontese, PM; Blonde d'Aquitaine, BA; Marchigiana, MA; Valdostana, VALD) and the potential transcriptional effect of the prototypical inducer phenobarbital (PB) upon the CYP3A isoforms was evaluated. Cytochrome P450 3A38 showed the highest amounts of gene copy numbers, followed by CYP3A48 and CYP3A28. Significant breed-differences in CYP3A gene abundances were found, and PB significantly up-regulated all the CYP3A isoforms. The data provide new information about CYP3A expression in cattle, particularly the heterogeneity in the pattern of expression of distinct hepatic CYP3As (CYP3A38 > 3A48 >> 3A28), the significant effect of breed, and their common up-regulation following the exposure to PB, although with different orders of magnitude.


PMID: 25193407

DOI: 10.1016/j.tvjl.2014.07.028

Other references

Pompeiano, O.; Wand, P.; Sontag, K.H., 1975: The relative sensitivity of Renshaw cells to orthodromic group Ia volleys caused by static stretch and vibrations of extensor muscles. 1. Activity of Renshaw cells monosynaptically excited by ventral root stimulation and disynaptically excited by electric stimulation of the group Ia afferents in the gastrocnemius-soleus (GS) nerve, was recorded in precollicular decerebrate cats....

Annandale, N., 1920: Report on the aquatic fauna of Seistan, with subsidiary studies. Part III. Note on the occurrence of the leech Limnatis nilotica in Seistan and the Afghan Baluch desert. Records of the Indian Museum, 18 135-136

Williams, C.L., 1891: Case of Persistent Hyaloid Artery. Indian Medical Gazette 26(12): 365-365

Dixon, S.E.; Bhatti, M.M.; Uversky, V.N.; Dunker, A.Keith.; Sullivan, W.J., 2011: Regions of intrinsic disorder help identify a novel nuclear localization signal in Toxoplasma gondii histone acetyltransferase TgGCN5-B. We have previously shown that protozoan parasites, such as Toxoplasma gondii, contain a high prevalence of intrinsically disordered regions in their predicted proteins. Here, we determine that both TgGCN5-family histone acetyltransferases (HATs) c...

Marriner, A., 1976: Evaluation of personnel. Supervisor Nurse 7(5): 36-39

Varga L., 1991: Odorvar channeling, southern Bukk Mountains. Foldrajzi Ertesito = Geographical Bulletin 40(1-2): 133-146

Oliver, L.R.; Klingman, T.E.; Eldrige, I.L., 1992: Influence of hairy vetch on weed control and soyabean yield. In field trials of soyabeans cv. Bedford in 1986-88 on clay, a comparison was made of 5 weed control treatments: no-tillage with hairy vetch, alone + imazaquin pre-em. and + imazaquin pre-em. followed by (FB) sethoxydim + crop oil FB acifluorfen +...

Leigh, P.G., 1968: Small vessel dissecting aneurysm with haemoperitoneum. South African Journal of Surgery. Suid-Afrikaanse Tydskrif Vir Chirurgie 6(2): 71-77

Brouwer, L.; Komdeur, J.; Richardson, D.S., 2007: Heterozygosity-fitness correlations in a bottlenecked island species: a case study on the Seychelles warbler. We used capture-mark-recapture models to investigate the effects of both individual and parental heterozygosity, measured at microsatellite loci on the survival of Seychelles warblers (Acrocephalus sechellensis), an endemic island species which w...

Umemoto, S.; Ose, S.; Takamatsu, H., 1963: Syntheses Of 1-Acyl-3-Phenylacetylurea Derivatives. Yakugaku Zasshi 83: 753-756