+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Activation and molecular recognition of the GPCR rhodopsin--insights from time-resolved fluorescence depolarisation and single molecule experiments

, : Activation and molecular recognition of the GPCR rhodopsin--insights from time-resolved fluorescence depolarisation and single molecule experiments. European Journal of Cell Biology 91(4): 300-310

The cytoplasmic surface of the G-protein coupled receptor (GPCR) rhodopsin is a key element in membrane receptor activation, molecular recognition by signalling molecules, and receptor deactivation. Understanding of the coupling between conformational changes in the intramembrane domain and the membrane-exposed surface of the photoreceptor rhodopsin is crucial for the elucidation of the molecular mechanism in GPCR activation. As little is known about protein dynamics, particularly the conformational dynamics of the cytoplasmic surface elements on the nanoseconds timescale, we utilised time-resolved fluorescence anisotropy experiments and site-directed fluorescence labelling to provide information on both, conformational space and motion. We summarise our recent advances in understanding rhodopsin dynamics and function using time-resolved fluorescence depolarisation and single molecule fluorescence experiments, with particular focus on the amphipathic helix 8, lying parallel to the cytoplasmic membrane surface and connecting transmembrane helix 7 with the long C-terminal tail.


PMID: 21803442

DOI: 10.1016/j.ejcb.2011.03.009

Other references

Boissard, R.; Fort, P.; Gervasoni, D.; Barbagli, B.; Luppi, P.H.rve, 2003: Localization of the GABAergic and non-GABAergic neurons projecting to the sublaterodorsal nucleus and potentially gating paradoxical sleep onset. We recently determined in rats that iontophoretic application of bicuculline or gabazine [two GABAa antagonists] and kainic acid (a glutamate agonist) in the sublaterodorsal nucleus (SLD) induces with a very short latency a paradoxical sleep-like...

Goeden, Richard, D., 2002: Life history and description of immature stages of Oxyna palpalis (Coquillett) (Diptera: Tephritidae) on Artemisia tridentata Nuttall (Asteraceae) in southern California. Oxyna palpalis (Coquillett) is a univoltine, circumnatal tephritid uniquely reproducing as an inquiline in rosette galls of Rhopalomyia florella Gagne (Diptera: Cecidomyiidae) of terminal buds on branches of Artemisia tridentata Nuttall. Its larva...

Ryumina I.I., 1967: Investigation of the molluscan fauna of the 'Vastyanskiy kon' outcrop, lower Pechora River. Pages 35-36 1967

Diaconu, I.; Vadineanu, A.; Risnoveanu, G., 1994: Changes of the structure and functioning of the benthic Oligochaete communities from the Danube Delta aquatic ecosystems 2 The assessment of their role on ecosystems productivity. This paper is dealing with the investigation of some aspects regarding energy flow in five lakes of the Danube Delta during 1976-1982 interval. The parameters of energy budget, the assimilation efficiency (U-1), the efficiency of utilization for g...

DeLORIMIER.A.A., 1951: Reflex hyperemic deossifications. Bulletin of the Hospital for Joint Diseases 12(2): 22-37

Hartlapp, J.H.; Kürten, J.; Gugler, R., 1978: Metabolism of clofibrate in hepatitis and liver cirrhosis. Verhandlungen der Deutschen Gesellschaft für Innere Medizin: 1108-1110

Roy, J.; Lin, X., 2000: Latent variable models for longitudinal data with multiple continuous outcomes. Multiple outcomes are often used to properly characterize an effect of interest. This paper proposes a latent variable model for the situation where repeated measures over time are obtained on each outcome. These outcomes are assumed to measure an...

DuBourdieu, D.J.; Kawaguchi, H.; Shier, W.T., 1987: Molecular weight variations in the diversity of phospholipase A2 forms in reptile venoms. Reptile venoms exhibit a wide diversity of phospholipase A2 forms when examined by electrophoretic and chromatographic techniques which separate on the basis of net charge. In principle, diversity in charge among the enzyme forms could result from...

Fleck, B.A.; Ling, N.; Chen, C., 2007: Substituted NDP-MSH peptides paired with mutant melanocortin-4 receptors demonstrate the role of transmembrane 6 in receptor activation. The melanocortin-4 receptor (MC4R) is involved in regulating energy homeostasis and is, a potential therapeutic target for obesity and cachexia. Molecular interactions between peptide ligands and MC4R have been studied in detail. Less is known re...

Sólyomváry, A.; Mervai, Z.; Molnár-Perl, I.; Boldizsár, I., 2014: Specific hydrolysis and accumulation of antiproliferative lignans in the fruit of Leuzea carthamoides (Willd.) DC. Dibenzylbutyrolactone-type lignan glycosides (tracheloside and carthamoside), their aglycones (trachelogenin and carthamogenin) and feruloyl-serotonin isomers were determined in the fruits of Leuzea carthamoides by using LC-UV, LC-MS/MS and GC-MS...