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Acute effects of the sigma-2 receptor agonist siramesine on lysosomal and extra-lysosomal proteolytic systems in lens epithelial cells


, : Acute effects of the sigma-2 receptor agonist siramesine on lysosomal and extra-lysosomal proteolytic systems in lens epithelial cells. Molecular Vision 16(): 819-827

The aim of the present study was to examine the effects of the sigma-2 receptor agonist, siramesine, on morphology, growth, cell death, lysosomal function, and effects on extra-lysosomal proteolytic systems in human lens epithelial cells. Human lens epithelial cells in culture were exposed to siramesine and examined for morphological changes using Nomarski optics or calcein. Lysosomes were evaluated using acridine orange and Magic Red (RR-cresyl violet). Nuclear morphology was studied using Hoechst 33342 and propidium iodide. Enzymatic activities in living cells or cell lysates were studied using fluorogenic substrates. Siramesine at low concentrations increased the cytoplasmic proteolytic activity of the proteasome and the calpain system. Effects were also observed with respect to lysosomal morphology, acidity and function. In addition, activation of caspase-3 and the appearance of nuclei with an apoptotic morphology was found. Siramesine at low concentrations affects lens epithelial cells with perturbations of the major proteolytic systems and lysosomal morphology, resulting in caspase activation and cell death. Siramesine may be a possible substance for the treatment or prevention of posterior capsular opacification (PCO).

US$29.90

PMID: 20461150


Other references

Justus Philip S.; Stamatakos John; Ferrill D.A.; Waiting D.J.; Morris A.P.; Sims D.W.; Ghosh A., 2004: Methods developed by the U. S. Nuclear Regulatory Commission to evaluate risk from fault displacements through a potential waste repository, Yucca Mountain, Nevada, USA. International Geological Congress, Abstracts = Congres Geologique International, Resumes 32, Part 1: 117

Cho E S.; Sharer L.R., 1990: Hiv encephalopathy in children. Kozlowski, P B , Et Al (Ed ) Brain in Pediatric Aids; Conference on Brain And Behavior in Pediatric Hiv Infection, New York, New York, Usa, July 24-25, 1989 Xv+204p S Karger Ag: Basel, Switzerland; New York, New York, Usa Illus 158-164

Anonymous, 2011: Lowering the color of crystals in sugar factories. Like diamonds, sugar crystals ideally are very pure and low in color. Now studies led by US Department of Agriculture chemist Gillian Eggleston have provided a better understanding of the source of undesirable color in factory sugar.

Hight-Laukaran, V.; Rutstein, S.O.; Peterson, A.E.; Labbok, M.H., 1996: The use of breast milk substitutes in developing countries: the impact of women's employment. Objectives: This study quantified the influence of employment, specifically a mother's employment away from her infant, on the use of breast milk substitutes in developing countries. Methods: Data from the Demographic and Health Surveys were...

Karhade, A.V.; Fandino, L.; Gupta, S.; Cote, D.J.; Iorgulescu, J.B.; Broekman, M.L.; Aglio, L.S.; Dunn, I.F.; Smith, T.R., 2016: Impact of operative length on post-operative complications in meningioma surgery: a NSQIP analysis. NlmCategory="UNASSIGNED">Many studies have implicated operative length as a predictor of post-operative complications, including venous thromboembolism [deep vein thrombosis (DVT) and pulmonary embolism (PE)]. We analyzed the American...

Tsurikisawa, N.; Oshikata, C.; Tsuburai, T.; Sugano, S.; Nakamura, Y.; Shimoda, T.; Tamama, S.; Adachi, K.; Horita, A.; Saito, I.; Saito, H., 2016: Th17 cells reflect colon submucosal pathologic changes in active eosinophilic granulomatosis with polyangiitis. Chronic eosinophilic pneumonia (CEP) or eosinophilic gastroenteritis (EG), or both, with asthma precede the onset of eosinophilic granulomatosis with polyangiitis (EGPA) in half of all EGPA patients. It is not known what determines whether patient...

Carter, M., 1967: C. Metabolism and mechanisms of 3-amino-1,2,4-triazole in higher plants. Metabolism of aminotriazole in higher plants appeared to be restricted to conjugation. In vitro studies showed little evidence of an aminoglucoside derivative in higher plants. Two conjugates reported in a variety of plants were identified as 3-(3...

Mohamed A.K.A.; Nelson F.R.S., 1984: Toxic effects of nomuraea rileyi extract on heliothis spp. Toxic extract produced in vitro in submerged culture was extracted from mycelia of N. rileyi (Farlow) Samson by methanol and chromatographed in columns of Ottawa sand and silica gel with benzene-ethyl acetate as the eluent. Third-instar H. Zea (Bo...

Burgwald Balstad, L.A.; Sanson, D.W.; Riley, M.L.; Gaebe, R.J.; Paisley, S.I.; Taylor, T.L., 1995: Effect of condition score of mature ewes on intake of alfalfa cubes. Journal of Animal Science 73(SUPPL 1): 339

Gulati, R.B.; Wadia, R.S.; Shaikh, R.A.; Soares, P.M.; Grant, K.B., 1977: Precordial mapping in acute myocardial infarction. Serial precordial mapping was done in 30 patients with acute anterior myocardial infarction, 27 transmural and 3 subendorcardial. The sum of ST elevations in the 48 lead map was designated as sigma ST. Normal sigma ST was calculated as 27.1+/-3.1...