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Adaptation in a mouse colony monoassociated with Escherichia coli K-12 for more than 1,000 days

, : Adaptation in a mouse colony monoassociated with Escherichia coli K-12 for more than 1,000 days. Applied and Environmental Microbiology 76(14): 4655-4663

Although mice associated with a single bacterial species have been used to provide a simple model for analysis of host-bacteria relationships, bacteria have been shown to display adaptability when grown in a variety of novel environments. In this study, changes associated with the host-bacterium relationship in mice monoassociated with Escherichia coli K-12 over a period of 1,031 days were evaluated. After 80 days, phenotypic diversification of E. coli was observed, with the colonizing bacteria having a broader distribution of growth rates in the laboratory than the parent E. coli. After 1,031 days, which included three generations of mice and an estimated 20,000 generations of E. coli, the initially homogeneous bacteria colonizing the mice had evolved to have widely different growth rates on agar, a potential decrease in tendency for spontaneous lysis in vivo, and an increased tendency for spontaneous lysis in vitro. Importantly, mice at the end of the experiment were colonized at an average density of bacteria that was more than 3-fold greater than mice colonized on day 80. Evaluation of selected isolates on day 1,031 revealed unique restriction endonuclease patterns and differences between isolates in expression of more than 10% of the proteins identified by two-dimensional electrophoresis, suggesting complex changes underlying the evolution of diversity during the experiment. These results suggest that monoassociated mice might be used as a tool for characterizing niches occupied by the intestinal flora and potentially as a method of targeting the evolution of bacteria for applications in biotechnology.


PMID: 20472724

DOI: 10.1128/AEM.00358-10

Other references

Boenisch R., 1989: Rhythm and symmetry in the second Lausitz seam horizon; a contribution to seam genesis. Zeitschrift fuer Angewandte Geologie 35(4): 106-110

Ninnemann J.L., 1978: Bone marrow derived cell activation of suppressor thymus derived cells in melanoma. Federation Proceedings 37(6): 1665

Gadir, A.E.A.; Khalil, K.M.; Rahman, M.M.A.; E.R.yah, I.E.; E.M.lik, K.H., 2006: Application of card agglutination test and card indirect-agglutination antigen test for detection of camel trypanosomosis in Western Sudan. A total of 81 blood samples from traditionally managed camels were examined parasitologically and serologically for the presence of camel trypanosomiasis in Northern and Southern Kordofan States in western Sudan. Parasitological examinations revea...

Kuzmina, O.; Bordes, E.; Schmauck, J.; Hunt, P.A.; Hallett, J.P.; Welton, T., 2016: Solubility of alkali metal halides in the ionic liquid [C4C1im][OTf. The solubilities of the metal halides LiF, LiCl, LiBr, LiI, NaF, NaCl, NaBr, NaI, KF, KCl, KBr, KI, RbCl, CsCl, CsI, were measured at temperatures ranging from 298.15 to 378.15 K in the ionic liquid 1-butyl-3-methylimidazolium trifluoromethanesulf...

Schaeffer, A.J., 2012: Editorial comment. Urology 79(3): 562; Author Reply 562-3

Agrawal, N.K., 1999: Fodder and fuel: from deficit to surplus. An afforestation project in Sakwa village, Gujarat, financially and technically supported by the Aga Khan Rural Support Programme-India but run by the village level organization, Gram Vikas Mandal, is described. Progress from 1989-95 is traced wit...

Anonymous, 1989: Geological excursion to Parnu District. Pages 16-22 1989

Bétrémieux, P.; Gold, F.; Parat, S.; Farnoux, C.; Rajguru, M.; Boithias, C.; Mahieu-Caputo, D.; Jouannic, J-M.; Hubert, P.; Simeoni, U., 2010: Implementing palliative care for newborns in various care settings. Part 3 of "Palliative care in the neonatal period". Palliative care in newborns may take place in the delivery room and then continued either in maternity wards or in the neonatal unit. For babies developing a chronic condition, going home may be advantageous. The population concerned includes babi...

Ward, D.J.; Chen, Y.; Platt, E.; Robson, B., 1991: Development and testing of protocols for computer-aided design of peptide drugs, using oxytocin. Considerable clinical interest in neuropeptides and peptide hormones has stimulated recent research and development of peptide-based drugs. This process differs from most classical drug discovery procedures because peptide molecules have considera...

Rogers, S.O.; Beaulieu, G.C.; Bendich, A.J., 1993: Comparative studies of gene copy number. Methods in Enzymology 224: 243-251