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Alphaherpesvirus axon-to-cell spread involves limited virion transmission


, : Alphaherpesvirus axon-to-cell spread involves limited virion transmission. Proceedings of the National Academy of Sciences of the United States of America 109(42): 17046-17051

The spread of viral infection within a host can be restricted by bottlenecks that limit the size and diversity of the viral population. An essential process for alphaherpesvirus infection is spread from axons of peripheral nervous system neurons to cells in peripheral epithelia (anterograde-directed spread, ADS). ADS is necessary for the formation of vesicular lesions characteristic of reactivated herpesvirus infections; however, the number of virions transmitted is unknown. We have developed two methods to quantitate ADS events using a compartmentalized neuronal culture system. The first method uses HSV-1 and pseudorabies virus recombinants that express one of three different fluorescent proteins. The fluorescence profiles of cells infected with the virus mixtures are used to quantify the number of expressed viral genomes. Strikingly, although epithelial or neuronal cells express 3-10 viral genomes after infection by free virions, epithelial cells infected by HSV-1 or pseudorabies virus following ADS express fewer than two viral genomes. The second method uses live-cell fluorescence microscopy to track individual capsids involved in ADS. We observed that most ADS events involve a single capsid infecting a target epithelial cell. Together, these complementary analyses reveal that ADS events are restricted to small numbers of viral particles, most often a single virion, resulting in a single viral genome initiating infection.

US$19.90

PMID: 23027939

DOI: 10.1073/pnas.1212926109


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Wehl, G.; Rossler, J.; Otten, J.E.; Boehm, N.; Uhl, M.; Kontny, U.; Niemeyer, C., 2004: Response of progressive fibromatosis to therapy with liposomal doxorubicin. Background: Patients with fibromatosis not amenable to surgery may suffer from high morbidity. Various chemotherapeutic regimens have been tried in these patients with limited success. Here, we report on the successful use of pegylated liposomal d...

Nolan, M.S.; Lindsey, N.J.; Savas, C.P.; Slater, D.N.; Fox, M., 1982: Pancreatic transplantation in the rat. A simplified method using aortic interposition and cuff techniques. Transplantation 33(3): 327-329

Iolevski, M.P.nkov, M.K.adzhiianakiev, A., 1974: An improved system for designati. Pochvoznanie i agrokhimieiia: (6) 112-116

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Callaghan, M.N., 1960: Haemo-lytic anemia associated with megaloblastic erythropoiesis. Three cases of hemolytic anemia associated with megaloblastic erythropoiesis are described. The megaloblastic condition was due to folic acid deficiency, vitamin B12 deficiency, and pernicious anemia respectively.

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