geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Alterations of protein profile in zebrafish liver cells exposed to methyl parathion: a membrane proteomics approach


, : Alterations of protein profile in zebrafish liver cells exposed to methyl parathion: a membrane proteomics approach. Chemosphere 87(1): 68-76

Methyl parathion (MP) is an extensively used organophosphorus pesticide, which has been associated with a wide spectrum of toxic effects on environmental organisms. The aim of this study is to investigate the alterations of membrane protein profiles in zebrafish liver (ZFL) cell line exposed to MP for 24 h using proteomic approaches. Two-dimensional gel electrophoresis revealed a total of 13 protein spots, whose expression levels were significantly altered by MP. These differential proteins were subjected to matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry analysis, and nine proteins were identified to be membrane proteins, among which seven were up-regulated, while two were down-regulated. In addition, the mRNA levels corresponding to these differential membrane proteins were further analyzed by quantitative real-time PCR. And the differential expression of arginase-2 was specially validated via Western blotting. Regarding the physiological functions, these proteins are involved in molecular chaperon, cytoskeleton system, cell metabolism, signal transduction, transport and hormone receptor respectively, suggesting the complexity of MP-mediated toxicity to ZFL cell. These data could provide useful insights for better understanding the hepatotoxic mechanisms of MP and develop novel protein biomarkers for effectively monitoring MP contamination level in aquatic environment.

US$19.90

PMID: 22182705

DOI: 10.1016/j.chemosphere.2011.11.061


Other references

Hancock, C., 1993: New dimensions in caring. Nursing the Elderly 5(1): 10-12

Bernstein, S.H.; Brunetto, V.L.; Davey, F.R.; Wurster-Hill, D.; Mayer, R.J.; Stone, R.M.; Schiffer, C.A.; Bloomfield, C.D., 1996: Acute myeloid leukemia-type chemotherapy for newly diagnosed patients without antecedent cytopenias having myelodysplastic syndrome as defined by French-American-British criteria: a Cancer and Leukemia Group B Study. Purpose: To determine the treatment out come of standard acute myeloid leukemia (AML)-type chemotherapy in a subset of patients with newly diagnosed myelodysplastic syndromes (MDS) compared with that of patients with de novo AML as defined using F...

Sannino, G.; Marchetto, A.; Kirchner, T.; Grünewald, T.G.P., 2017: Epithelial-to-Mesenchymal and Mesenchymal-to-Epithelial Transition in Mesenchymal Tumors: A Paradox in Sarcomas?. The epithelial-to-mesenchymal transition (EMT) is a reversible process comprised of various subprograms via which epithelial cells reduce their intercellular adhesions and proliferative capacity while gaining a mesenchymal phenotype with increased...

Westin, B.; Parentela, A.; Ziliotto, D.; Odeblad, E., 1956: Na24 clearance in hypo- and hyperthermic rats. Acta Chirurgica Scandinavica 110(4): 316-318

Yan, Z.X.; Chen, D.Y.; Sun, T.; Zhang, H.M., 1985: A preliminary report of study on nuclear polyhedrosis viruses in Parasa consocia (Lep.: Cochlidiidae). A nuclear polyhedrosis virus was isolated from larvae of the limacodid Parasa consocia. The polyhedra were 1.16-2.1 micro m in diameter, and its particles were rod-shaped, 383.6 x 58.6 nm, with 2-3 rods in a bundle. The virus belonged to subgroup...

Taghzouti, K.; Simon, H.; L.M.al, M., 1986: Disturbances in exploratory behavior and functional recovery in the Y and radial mazes following dopamine depletion of the lateral septum. The effects of 6-hydroxydopamine (6-OHDA) injected into the lateral septum in rats were investigated for spontaneous alternation behavior in a Y maze and for spatially oriented behavior in an 8-arm radial maze. The performance of the animals in th...

Hsieh WeinChang; Chen JangFu; Cheng YuKuei, 2002: Effects of different growth period and grass ensiling on oxalate content and quality of napiergrass. The purpose of the study is to determine the effects of different growth seasons, cutting stages and grass ensiling on oxalate content of napier grass. Results shows that napier grass had higher oxalate content in summer and low content during win...

Ruff-Jamison, S.; Campos-González, R.; Glenney, J.R., 1991: Heavy and light chain variable region sequences and antibody properties of anti-phosphotyrosine antibodies reveal both common and distinct features. Phosphotyrosine and similar analogs have been used to elicit antibodies that have found widespread use in the study of cellular tyrosine phosphorylation. In order to better understand the anti-phosphotyrosine immune response and to elucidate the d...

Ciadamidaro, S.; Mancini, L.; Rivosecchi, L., 2016: Black flies (Diptera, Simuliidae) as ecological indicators of stream ecosystem health in an urbanizing area (Rome, Italy). The effectiveness of Simuliidae (Diptera, Nematocera) as indicators of stream health was tested in the urbanizing area of Rome, Central Italy. 108 sampling sites were investigated in the spring to acquire data on environmental variables and black...

Pastrana B.; Nieves I.; Lopez C.; Santiago G., 1986: Determination of binding constant between vanadyl and calmodulin by esr. Abstracts of Papers American Chemical Society 192