+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Alternating RF/DC Isolations for Quantitation with Coeluting Internal Standards in Gas Chromatography/Ion Trap Mass Spectrometry

, : Alternating RF/DC Isolations for Quantitation with Coeluting Internal Standards in Gas Chromatography/Ion Trap Mass Spectrometry. Journal of the American Society for Mass Spectrometry 3(1): 85-88

A new ion trap scan function for gas chromatography/mass spectrometry (GC/MS) quantitation is described that employs alternating mass-selective storage (rf/dc isolation) of ions from an analyte and its coeluting isotopically labeled internal standard. This scan includes two separate ionization/isolation/mass analysis sequences within the same scan function, each optimized for either the analyte or the internal standard. This results in alternating between analyzing the analyte and the internal standard during their coelution. The method is conceptually similar to using two different scan functions to analyze either the analyte or the internal standard in alternating scans; however, it is much faster because it eliminates the slow procedure of continuously downloading alternating scan functions from disk. This allows more data points to be obtained over a GC peak, resulting in more reproducible GC peak profiles as well as better sensitivity and precision. Results of calibration curves spanning four orders of magnitude (0.5 pg to 5 pg injected on column) obtained by using this method give excellent linear correlations (r (2) > 0.9990) and precision (relative standard deviations of triplicate injections < 10%).


PMID: 24242841

DOI: 10.1016/1044-0305(92)85022-C

Other references

Shkorbatov, G.L., 1964: The effects of environmental temperatures on the activity of blood catalase and liver dehydrase in carp, Amur carp and their hybrids. Blood catalase activity was determined at 2 to 50[degree]C, and liver catalase activity at 10 to 50[degree]. Maximum catalase activity was observed at 20[degree]. In carp, a thermophilic form, the catalase activity was 71% at 10[degree] (taking th...

Varghese, Z.; Moorhead, J.F.; Tatler, G.L.; Baillod, R.A.; Wills, M.R., 1973: Plasma hydroxyproline in renal osteodystrophy. Proceedings of the European Dialysis and Transplant Association. European Dialysis and Transplant Association 10(0): 187-196

Saleh, K.; Bessei, W., 1981: The genetics of locomotor activity in quails. In 7-wk-old male and female Japanese quails belonging to 3 lines, and reciprocal crosses of 2 of the lines, the h2 of locomotor activity in single cages was 0.31, and that of body weight 0.35, both estimated from full-sib correlations. The genetic...

Sibbald, B., 2006: New Day dawns. Cmaj 175(6): 566-566

Kulapaditharom, B.; Boonkitticharoen, V., 1998: Laser-induced fluorescence imaging in localization of head and neck cancers. Laser-induced fluorescence tumor imaging exploits the difference in tissue autofluorescence properties between normal and cancerous tissues. The effectiveness and reliability of fluorescence imaging with a lung imaging fluorescence endoscopy (LIFE...

Tsukada, M.; Sheng, H.; Kamachi, T.; Niwano, Y., 2016: Microbicidal action of photoirradiated aqueous extracts from wine lees. NlmCategory="UNASSIGNED">Wine lees, a major waste product of winemaking, is a rich source of polyphenolic compounds. LED-light irradiation at 400-nm elicited microbicidal activity of aqueous extract from wine lees (WLE) against <i&...

Polyakov, A.A., 1956: In the USA for the antibiotics conference Referat Zhur, Biol, 1957, No 93. Detailed reports of the papers related to the use of antibiotics in animal husbandry.

Sandvik, A.W., 2003: Stochastic series expansion method for quantum Ising models with arbitrary interactions. A quantum Monte Carlo algorithm for the transverse Ising model with arbitrary short- or long-range interactions is presented. The algorithm is based on sampling the diagonal matrix elements of the power-series expansion of the density matrix (stoc...

Amoedo Mon, M., 1959: Polycystic kidney: review of the literature & report of 4 cases. Archivos. Hospital Universitario General Calixto Garcia 11(1): 24-33

Soulairac, A., 1959: Psycho-physiological regulation of hunger. Lyon Medical 91(11): 470-472