+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

An injectable oxidated hyaluronic acid/adipic acid dihydrazide hydrogel as a vitreous substitute

, : An injectable oxidated hyaluronic acid/adipic acid dihydrazide hydrogel as a vitreous substitute. Journal of Biomaterials Science. Polymer Edition 22(13): 1777-1797

Vitrectomy is a common procedure for treating ocular-related diseases. The surgery involves removing the vitreous humor from the center of the eye, and vitreous substitutes are needed to replace the vitreous humor after vitrectomy. In the present study, we developed a colorless, transparent and injectable hydrogel with appropriate refractive index as a vitreous substitute. The hydrogel is formed by oxidated hyaluronic acid (oxi-HA) cross-linked with adipic acid dihydrazide (ADH). Hyaluronic acid (HA) was oxidized by sodium periodate to create aldehyde functional groups, which could be cross-linked by ADH. The refractive index of this hydrogel ranged between 1.3420 and 1.3442, which is quite similar to human vitreous humor (1.3345). The degradation tests demonstrated that the hydrogel could maintain the gel matrix over 35 days, depending on the ADH concentration. In addition, the cytotoxicity was evaluated on retina pigmented epithelium (RPE) cells cultivated following the ISO standard (tests for in vitro cytotoxicity), and the hydrogel was found to be non-toxic. In a preliminary animal study, the oxi-HA/ADH hydrogel was injected into the vitreous cavity of rabbit eyes. The evaluations of slit-lamp observation, intraocular pressure, cornea thickness and histological examination showed no significant abnormal biological reactions for 3 weeks. This study suggests that the injectable oxi-HA/ADH hydrogel should be a potential vitreous substitute.


PMID: 20843434

DOI: 10.1163/092050610X522729

Other references

Brändén, C.I.; Eklund, H., 1977: Coenzyme-induced conformational changes and substrate binding in liver alcohol dehydrogenase. The apoenzyme and holoenzyme structures of liver alcohol dehydrogenase have been determined by X-ray methods to obtain details about coenzyme binding, substrate specificity and the catalytic mechanism. Coenzyme binding induces a conformational cha...

Armstrong, S., 2016: What happens to data gathered by health and wellness apps?. Bmj 353: I3406-I3406

Beetz, Kj, 1970: Experiences with the growth regulator CCC in vines which have already borne fruit. Weinberg und Keller: 17 (9) 413-426

Lodos N., 1968: Studies on cocoa d pod pentatomid bathycoelia thalassina. Cocoa Research Institute (Ghana Academy of Sciences) Annual Report : 1969, 1967

Negus, V.E., 1931: Atrophic OEsophagitis in Region of Crico-pharyngeal Fold. Proceedings of the Royal Society of Medicine 24(9): 1209-1210

Anonymous, 1994: Cream resists the recession. The German market for cream has proved resistant compared with the downward trends in the consumption of all milk products during 1993. Amounts of whipping cream, creme fraiche and double cream sold to consumers have increased by just over 5%. Hou...

Messner, G.N.; Gregoric, I.D.; Chu, T.; Radovancevic, B.; Kar, B.; Flamm, S.D.; Frazier, O.H., 2006: Orthotopic heart transplantation in a patient with D-transposition of the great arteries: after a Mustard procedure. During heart transplantation, the atrial septum is most often used to reconstruct the right atrial reservoir. We report the case of a patient with corrected D-transposition of the great arteries who underwent successful cardiac transplantation des...

Kimura, H.; Sohmura, T.; Watanabe, T., 1989: Three dimensional shape measurement of teeth (Part 2). The accuracy of the three dimensional measurement using a laser displacement meter. The three dimensional shape measurement system of teeth was improved. The system was assembled by using a high precision laser displacement meter and computer controlled scanning machine (CAMM-3). At the fissure of the occlusal plane, the abnormal...

Li, D.; Rozen, T.D., 2002: The clinical characteristics of new daily persistent headache. New daily persistent headache (NDPH) is a subtype of chronic daily headache. The literature on NDPH is scant and its true aetiology is unknown. A retrospective chart review was carried out from a computerized database at the Jefferson Headache Cen...

Wang, Y.Y.; Thani, N.B.; Han, T.F., 2010: Optic nerve penetration by a carotico-ophthalmic artery aneurysm. Fenestration of a cranial nerve by an internal carotid artery aneurysm is rarely identified at surgical repair of intracranial aneurysms. Classically this condition is associated with visual disturbance in either acuity, fields or diplopia. We des...