geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Analysis of a unique interaction between the complement regulatory protein factor H and the periodontal pathogen Treponema denticola


, : Analysis of a unique interaction between the complement regulatory protein factor H and the periodontal pathogen Treponema denticola. Infection and Immunity 77(4): 1417-1425

Treponema denticola, a spirochete associated with periodontitis, is abundant at the leading edge of subgingival plaque, where it interacts with gingival epithelia. T. denticola produces a number of virulence factors, including dentilisin, a protease which is cytopathic to host cells, and FhbB, a unique T. denticola lipoprotein that binds complement regulatory proteins. Earlier analyses suggested that FhbB specifically bound to factor H (FH)-like protein 1 (FHL-1). However, by using dentilisin-deficient mutants of T. denticola, we found that T. denticola preferentially binds FH and not FHL-1, and that FH is then cleaved by dentilisin to yield an FH subfragment of approximately 50 kDa. FH bound to dentilisin-deficient mutants but was not cleaved and retained its ability to serve as a cofactor for factor I in the cleavage of C3b. To assess the molecular basis of the interaction of FhbB with FH, mutational analyses were conducted. Replacement of specific residues in widely separated domains of FhbB and disruption of a central alpha helix with coiled-coil formation probability attenuated or eliminated FH binding. The data presented here are the first to demonstrate the retention at the cell surface of a proteolytic cleavage product of FH. The precise role of this FH fragment in the host-pathogen interaction remains to be determined.

US$19.90

PMID: 19204088

DOI: 10.1128/IAI.01544-08


Other references

Crapo R.O.; Crapo J.D.; Morris A.H., 1980: Comparison of morphometric and gas re breathing measurements of lung tissue volume. Clinical Research 28(1): 57A

Elmer, H.E., 1934: Early Diagnosis of Primary Syphilis : Practical Darkfield Examination by Mail. American Journal of Public Health and the Nation's Health 24(6 Pt 1): 629-630

Kader, M.; Bhuiyan, A.; Manzur, A.; Khuda, I., 1988: The reproductive biology of Gobioides rubicundus (Ham. Buch.) in the Karnaphuli River Estuary Chittagong. Indian Journal of Fisheries 35(4): 239-250

Chaturvedi; Sandhya; Srinivasan; Simon, 1990: Thermal counterparts of nonclassical states in quantum optics. Physical Review. A, Atomic, Molecular, and Optical Physics 41(7): 3969-3974

Ferris, C.D., 2006: Taxonomic note on Eupithecia cupressata Pearsall (Lepidoptera: Geometridae: Eupitheciini). The male of Eupithecia cupressata Pearsall is described, and illustrations of adults and genitalia of both sexes are provided.

Burek, M.J.; Nordeen, K.W.; Nordeen, E.J., 1994: Ontogeny of sex differences among newly-generated neurons of the juvenile avian brain. In zebra finches, only males sing and brain regions controlling song exhibit sex differences in neuron number that stem from actions of estrogen during a critical developmental period. In certain song nuclei, these dimorphisms emerge long after ne...

Smith, N.L.; Chan, J.D.; Rea, T.D.; Wiggins, K.L.; Gottdiener, J.S.; Lumley, T.; Psaty, B.M., 2004: Time trends in the use of beta-blockers and other pharmacotherapies in older adults with congestive heart failure. Background Evidence supporting pharmacotherapy of congestive heart failure (CHF) has grown substantially over the past decade and includes large, placebo-controlled trials with mortality end points. We describe beta-blocker and other medication te...

Villalobos, G.H.; Vargas, Aélica.M.; Rondón, M.A.; Felknor, S.A., 2013: Design of psychosocial factors questionnaires: a systematic measurement approach. Evaluation of psychosocial factors requires instruments that measure dynamic complexities. This study explains the design of a set of questionnaires to evaluate work and non-work psychosocial risk factors for stress-related illnesses. The measurem...

Wuest Georg, 1938: Die Grossgliederung des atlantischen Tiefseebodens. Major morphologic features of the Atlantic ocean floor.

Mattout-Drubezki, A.; Gruenbaum, Y., 2003: Dynamic interactions of nuclear lamina proteins with chromatin and transcriptional machinery. The nuclear lamina is a filamentous nuclear structure intimately connected to the inner nuclear membrane. It is composed of lamins, which are also present in the nuclear interior, and lamin-associated proteins. The nuclear lamina is involved direc...