geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Analysis of clinical characteristics and S gene mutation of hepatitis B virus (HBV) in patients with hepatitis B surface antigen RIA negative and HBV DNA positive


, : Analysis of clinical characteristics and S gene mutation of hepatitis B virus (HBV) in patients with hepatitis B surface antigen RIA negative and HBV DNA positive. Korean Journal of Laboratory Medicine 29(3): 224-230

We investigated hepatitis B virus (HBV) infection cases, who were HBsAg negative by radioimmunoassay (RIA) and HBV DNA positive for their clinical characteristics, the S gene mutation of hepatitis B virus (HBV), and usefulness of other HBsAg immunoassay. Among the patients requested for HBV DNA quantification, 16 patients positive in HBV DNA but negative in HBsAg RIA (BNIBT HBsAg Kit, China) were enrolled. The "a" determinant of HBV S gene was sequenced and clinical characteristics were reviewed. Additional HBsAg assay was performed using Architect HBsAg kit (Abbott laboratories, USA) employing chemiluminescent immunoassay method. Eleven of the 16 patients showed multiple mutations in the "a" determinant. These patients received liver transplantation several years ago and have been treated with hepatitis B immune globulin (HBIG) and antiviral drugs. G145R mutation was found in 8 patients and G145K, D144G, and D144A were also frequently found. Among 9 of the 11 patients tested for HBsAg by Architect HBsAg kit, 8 showed positive results. Among 4 of the remaining 5 patients, only 2 showed weak positive results (< or = 1 IU/mL) in Architect HBsAg kit. HBV DNA-positive/HBsAg RIA-negative results were mostly observed in the patients treated with HBIG after liver transplantation, in whom HBIG escape mutations were found. Majority of these cases were positive in Architect HBsAg assay, and it is recommended to use other HBsAg immunoassay methods that are more sensitive than RIA in the detection limit as well as in the detection of escape mutant in hospitals performing liver transplantation.

US$19.90

PMID: 19571620

DOI: 10.3343/kjlm.2009.29.3.224


Other references

Jeppsson Lennart, 1996: Silurian events; a review of the knowledge in 1996. International Symposium on the Silurian System Program and Abstracts

Waser, P.G.; Osterwalder, M.; Schoenenberger, E., 1978: Auto radiography of carbon 14 choline uptake in end plates and skeletal muscle of mice. The uptake of 14C-choline into the axonal part of the motor endplate and muscle of mouse diaphragms was investigated by autoradiography. With i.v. doses of 0.1 .mu.g/g choline chloride, the uptake into the nerve endings was fast (< 2 min) and i...

Rădulescua, A.; Adams, P., 2014: Hebbian crosstalk and input segregation. Hebbian synapses respond to input/output correlations, and thus to input statistical structure. However, recent evidence suggests that strength adjustments are not completely connection-specific, and this "crosstalk" could distort, or ev...

Martynova, O.M., 1957: Les Mecopteres de la faune de l'URSS. II. Fam. Panorpidae. Entomologicheskoe Obozrenie, 36: 721-747

Freese, U.E.; Maciolek, B.J., 1969: Plastoid injection studies of the uteroplacental vascular relationship in the human. Obstetrics and Gynecology 33(2): 160-169

Adily, A.; Ward, J.E., 2005: Enhancing evidence-based practice in population health: staff views, barriers and strategies for change. To determine barriers and enablers for evidence-based practice (EBP) in population health and potential strategies for change. Self-administered survey of 104 professional staff (response rate, 73%) in the Division of Population Health, South West...

Gerasimenko V.N.; Tkhostov A.Sh; Artyushenko Y.V., 1981: Psychological rehabilitation of oncologic patients. On the basis of results of psychological studies of cancer patients, typical pathopsychological disturbances and their impact on the rehabilitation process are described. Patient's awareness of his illness, methods of psychological defense, a...

Ichinoe K., 1977: Changes brought about in blood coagulation in the new born by administration of vitamin k 2. Thrombosis & Haemostasis: 267

Burton, A.K.; Tillotson, K.M.; Main, C.J.; Hollis, S., 1995: Psychosocial predictors of outcome in acute and subchronic low back trouble. Study Design. A prospective survey of patients seeking primary care for low back pain. clinical and psychosocial data, available at presentation, were explored for predictors of outcome at 1 year. Objectives. To determine the relative value of cli...

Thomas, J.M.; Lopez, J.Tessa., 2004: Conjoined twins--the anaesthetic management of 15 sets from 1991-2002. The Red Cross War Memorial Children's Hospital, Cape Town, South Africa is the only dedicated children's hospital in sub-Saharan Africa and, as such, is the referral hospital for complex procedures from this region. Fifteen sets of conjo...