geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Angiopoietin-1 induces neurite outgrowth of PC12 cells in a Tie2-independent, beta1-integrin-dependent manner


, : Angiopoietin-1 induces neurite outgrowth of PC12 cells in a Tie2-independent, beta1-integrin-dependent manner. Neuroscience Research 64(4): 348-354

Overexpression of angiopoietin (Ang) 1 in the brain results in increased vascularization and altered neuronal dendrite configuration. We hypothesized that Ang1 acts directly on neurons inducing neurite outgrowth. We stimulated PC12 cells with Ang1 and observed outgrowth levels comparable to nerve growth factor (NGF). Western blotting and RT-PCR demonstrated the absence of the Ang1 receptor, Tie2 and the presence of beta1-integrin. Downstream of beta1-integrin, Ang1 stimulation led to a approximately 2.6 fold increase in focal adhesion kinase (FAK) phosphorylation and no change in the activation of mitogen-activated protein kinase (MAPK) nor c-Jun N-terminal kinase (JNK). Conversely, NGF stimulation had no effect on FAK phosphorylation but led to a approximately 3.1 and approximately 2 fold increase in phosphorylation of MAPK and JNK. Ang1, but not NGF-mediated outgrowth was attenuated following functional inhibition of beta1-integrin and FAK, and Wortmannin inhibited neurite outgrowth mediated by both. Our results suggest that Ang1 induces neurite outgrowth in PC12 cells in a Tie2-independent, beta1-integrin-FAK-PI3K-Akt-dependent manner and that NGF and Ang1 mediate neurite outgrowth via two independent signaling mechanisms.

US$19.90

PMID: 19379779

DOI: 10.1016/j.neures.2009.04.007


Other references

Guern, N., 1982: Changes in sensitivity to heat of isolated tobacco cells cultured at 26 deg C. Sensitivity to heat was estimated by degree of permeability to erythrosine B of cells of tobacco cv. Wisconsin cultured for 40 days at 26 deg C. Sensitivity varied widely according to cell age and increased during active division with a max. just...

Bierman, H.R.; Larsen, V.R., 1946: Reactions of the human to impact forces revealed by high speed motion picture technique. Journal of Aviation Medicine 17(5): 407-412

Budai D.; Kasa P., 1985: Trifluoperazine decreases the hippocampal acetylcholine turnover rate. Journal of Neurochemistry 44(SUPPL): S104

Schmidtlein, S.; Ewald, J., 2003: Landscape patterns of indicator plants for soil acidity in the Bavarian Alps. Aim Electronic distribution atlases and lists of ecological indicator values are becoming important tools in plant geography. In this contribution, we combine a geographical and an ecological data bank, and map out patterns of indicator value spec...

Murata, K.; Yajima, H.; Maegawa, N.; Hattori, K.; Takakura, Y., 2008: Investigation of segmental carpal tunnel pressure in patients with idiopathic carpal tunnel syndrome--is it necessary to release the distal aponeurotic portion of the flexor retinaculum in endoscopic carpal tunnel release surgery?. Segmental carpal tunnel pressure was measured in 12 hands of 11 idiopathic carpal tunnel syndrome patients before and after two-portal endoscopic carpal tunnel release. We aimed to determine at which part of the carpal tunnel the median nerve coul...

Roberts, P.J.; Toms, N.J., 1994: Cyclic AMP enhances NMDA receptor-mediated stimulation of rat cerebellar nitric oxide formation. British Journal of Pharmacology 111(PROC SUPPL JAN ): 57P

Heier, A.; Gröne, A.; Völlm, J.; Kübber-Heiss, A.; Bacciarini, L.N., 2003: Immunohistochemical study of retinol-binding protein in livers of polar bears (Thalarctos maritimus). Liver tumours of unknown cause have frequently been described in polar bears. Concurrent decrease of vitamin A levels and chronic liver disease are associated with hepatic carcinogenesis in humans. More than 90% of the body's vitamin A is sto...

Torrenti, L.Amez, C., 1955: A rare tumor of the shoulder. Bordeaux Chirurgical: 173-174

Fletcher, J.J., 2002: Virtues, moral decisions, and health care. Nursingconnections 12(4): 26-32

Coscolla Ramon, R., 1981: Algunas observaciones sobre el pteromalido Dibrachys affinis Masi, parasito de Lobesia botrana Den y Schiff polilla del racimo de la vid. Boletin Servicio de Defensa contra Plagas e Inspeccion Fitopatologica, 71-2: 57-63