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Anticancer drugs exert differential apoptotic and cytotoxic effects on glioblastoma primary cultures with various EGFR and bcl-2 profiles


, : Anticancer drugs exert differential apoptotic and cytotoxic effects on glioblastoma primary cultures with various EGFR and bcl-2 profiles. Journal of Experimental Therapeutics & Oncology 8(2): 105-116

The aim of this study was to determine the apoptotic and cytotoxic effects induced on glioblastoma cells by various anticancer agents that possess different mechanisms of action (alkylating drugs, anti-EGFR (Epidermal Growth Factor receptor), proteasome inhibitor). Primary cell cultures were obtained from patients who underwent surgery for their glioblastoma. The cytotoxic effects of drugs were determined by MTT (dimethylthiazolyl diphenyl tetrazolium bromide) assay and apoptosis was evaluated by measuring mitochondrial potential by flow cytometry. Biological markers (EGFR, bcl-2) were studied by a immunoblotting technique to find out predictive markers of response. We found a large interindividual sensitivity, thus confirming the interest of the primary cultures. New proteasome inhibitor bortezomib had considerable cytotoxic and apoptotic potential in glioblastoma, even at very low concentrations. Moreover, the characterization of patients' cells for EGFR and bcl-2 status could constitute an interest, with the evaluation of other markers, in the study of expected chemotherapy response.

US$29.90

PMID: 20192117


Other references

Dzulkifli, M.A.; Sharpe, H.L.; Wilding, E.L., 2004: Separating item-related electrophysiological indices of retrieval effort and retrieval orientation. The relationship between two classes of retrieval process-retrieval orientation and retrieval effort-was investigated using electrophysiological (ERP) and behavioural measures. ERPs were recorded during retrieval phases of exclusion tasks in which...

Taylor, K., 2000: The clinical e-mail explosion. The computer is transforming patient-physician communication. Physicians are already using electronic mail (e-mail) in physician-to-physician consultation, medical journal dissemination, and hospital-physician communication. This article addresses...

Munoz Batet, J.; Lopez-Colon, J.I.nacio, 1999: Third capture of Ochodaeus inermis Reitter, 1893 in Catalonia. Boletin de la SEA 25 (Junio): 30

Parr, C.J.; Sharma, R.; Garber, P.J., 2017: Apical hypertrophic cardiomyopathy treated as ST-elevation myocardial infarction. Electrocardiographic changes resulting from apical hypertrophic cardiomyopathy may mimic an acute coronary syndrome. A 67-year-old Sudanese male without cardiac risk factors presented to hospital with chest pain and electrocardiographic findings o...

Moll, H., 1967: Medical first aid in child accidents. Der Landarzt 43(8): 341-346

Cohen, C., 2009: Low-level viremia in HIV-1 infection: consequences and implications for switching to a new regimen. Virologic failure, or the inability to maintain or achieve viral suppression below detectable limits (<50 copies/mL), occurs in some patients with human immunodeficiency virus (HIV)-1 infection, despite being on a potent antiretroviral (ARV) re...

Naujoks-Heinrich, S.; Dölle, W., 1982: Epidemiology of the most common tumors of the gastrointestinal tract. Der Internist 23(1): 1-9

Castell C.H.; Smith B.; Dyer W.J., 1974: Simultaneous measurements of tri methylamine and di methylamine in fish and their use for estimating quality of frozen stored gadoid fillets. Journal of the Fisheries Research Board of Canada 31(4): 383-389

VanderHoof Vertress Lawrence, 1933: Pliocene vertebrate fauna from Sierra Foothills of central California. Pan-American Geologist 59(5): 376-377

Chang, C.C.; Huang, S.Y., 2005: Eikenella corrodens arthritis of the knee after a toothpick injury: Report of one case. This is a case of an 11-year-old boy who developed septic arthritis of the right knee after a stabbing injury by a toothpick. Arthroscopic debridement of the distal femur and arthrotomy with synovectomy were performed at two hospitals. Cultures fr...