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Antimalarial and safety evaluation of Pluchea lanceolata (DC.) Oliv. & Hiern: in-vitro and in-vivo study

, : Antimalarial and safety evaluation of Pluchea lanceolata (DC.) Oliv. & Hiern: in-vitro and in-vivo study. Journal of Ethnopharmacology 149(3): 797-802

Many of the effective therapeutic strategies have been derived from ethnopharmacologically used natural products. Pluchea lanceolata is an herb employed in Indian folk medicine for malaria like fever but it lacks proper pharmacological intervention. To evaluate antimalarial and safety profile of Pluchea lanceolata: an in-vitro, in-vivo for its ethnopharmacological validation. Methanol, butanol, ethyl acetate, chloroform, hexane extracts and its isolate, taraxasterol acetate (TxAc) were obtained from air dried aerial part of Pluchea lanceolata. These were tested in-vitro against chloroquine-sensitive strain of Plasmodium falciparum NF54 by measuring the parasite specific lactate dehydrogenase activity. The most potent hexane extract and TxAc were further validated for in-vivo antimalarial and safety evaluation. TxAc, a pentacyclic-triterpene isolated from the most active fraction was further evaluated with special emphasis on inflammatory mediators involved in malaria pathogenesis. Murine malaria was induced by intra-peritoneal injection of Plasmodium berghei infected red blood cells to the male Swiss inbred mice. Mice were orally treated following Peters 4-Day suppression test. In-vivo antimalarial efficacy was examined by evaluating the parasitaemia, percent survival, mean survival time, blood glucose, haemoglobin and pro-inflammatory mediators involved in malaria pathogenesis. Hexane extract and TxAc showed promising antimalarial activity in-vitro and in-vivo condition. TxAc attributed in inhibition of the pro-inflammatory cytokines as well as afford to significant increase in the blood glucose and haemoglobin level when compared with vehicle treated infected mice. We have not observed the synergistic action of combinations of chloroquine and TxAc from our experimental results. In-vitro and in-vivo safety evaluation study revealed that hexane extract is non toxic at higher concentration. Present study further validates the ancient Indian traditional knowledge and use of Pluchea lanceolata as an antimalarial agent. Study confirms the suitability of Pluchea lanceolata as a candidate for further studies to obtain a prototype for antimalarial medicine.


PMID: 23954323

DOI: 10.1016/j.jep.2013.08.003

Other references

Zhang, J.; Chen, X.Guang.; Peng, W.Bing.; Liu, C.Sheng., 2008: Uptake of oleoyl-chitosan nanoparticles by A549 cells. The aim of the present study was to evaluate cellular uptake of oleoyl-chitosan (OCH) nanoparticles by using A549 cells, a human lung carcinoma cell line, for drug and gene delivery applications. In this study, self-assembled OCH nanoparticles enc...

Blanc Jouvan F.; Rose Pitet L.; Brichon P.Y.; Brambilla E.; Coulomb M.; Latreille R.; Sarrazin R.; Paramelle B.; Brambilla C., 1987: Comparison of magnetic resonance imaging mri and computed tomography ct for the assessment of lymph node metastasis and tumoral extension of bronchial carcinoma in 61 patients. American Review of Respiratory Disease 135(4 PART 2): A241

Bhargava, M.; Becker, T.L.; Viken, K.J.; Jagtap, P.D.; Dey, S.; Steinbach, M.S.; Wu, B.; Kumar, V.; Bitterman, P.B.; Ingbar, D.H.; Wendt, C.H., 2015: Proteomic profiles in acute respiratory distress syndrome differentiates survivors from non-survivors. Acute Respiratory Distress Syndrome (ARDS) continues to have a high mortality. Currently, there are no biomarkers that provide reliable prognostic information to guide clinical management or stratify risk among clinical trial participants. The obj...

Lin, C.C.; Kao, E.L., 1988: Bronchial arteriographic evaluation and surgical treatment of pulmonary aspergillosis. This report is of ten cases experience in bronchial arteriographic evaluation and surgical treatment of pulmonary aspergillosis at Kaohsiung medical College Hospital from January 1987 to June 1988. Although hemoptysis was a high challenge in the s...

Malmström, S.; Hannoun, C.; Lindh, M., 2007: Mutation analysis of lamivudine resistant hepatitis B virus strains by TaqMan PCR. Hepatitis B virus infected patients on long-term lamivudine treatment are exposed to a 15-32% risk compounded annually of developing resistance mutations. Such resistance results in a progression of the liver damage caused by chronic hepatitis B,...

Zhu, Y.Fang.; Wang, X-Yu.; Lowie, B-Jo.; Parsons, S.; White, L.; Kunze, W.; Pawelka, A.; Huizinga, J.D., 2015: Enteric sensory neurons communicate with interstitial cells of Cajal to affect pacemaker activity in the small intestine. Enteric sensory neurons (the AH neurons) play a role in control of gastrointestinal motor activity; AH neuron activation has been proposed to change propulsion into segmentation. We sought to find a mechanism underlying this phenomenon. We formula...

Eskov, K., 1984: A new fossil spider family from the Jurassic of Transbaikalia Araneae Chelicerata. Neues Jahrbuch fuer Geologie und Palaeontologie Monatshefte, 11: 645-653

Tanraev, A.D.; Aubakirov, S.A., 1982: Eradication of sheep helminthiases at a farm. Control programmes were implemented between 1976 and 1980 against gastrointestinal nematodes and cestodes and against larval cestode infections at a sheep farming complex in the Tselinograd region, USSR. Therapeutic and chemoprophylactic treatment...

Rees, M., 1993: Null models and dispersal distributions: a comment on an article by Caley. American Naturalist 141(5): 812-815

Gross S.S.; Kobolev V.P.; Kozlenko Y.V.; Korchagin I.N.; Solov yev V.D.; Yakimchuk N.A., 1999: Algorithms and processing programs for data interpretation of marine gradientometric measurement of magnetic field. The problem of computer registration, processing and interpreting of marine magnetometric measurements is considered. The computer technology of the registration and a set of programs of the in-line processing of the data of the magnetic field mea...