geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Antimicrobial peptides as new recognition molecules for screening challenging species


, : Antimicrobial peptides as new recognition molecules for screening challenging species. Sensors and Actuators. B, Chemical 121(1): 150-157

The goal of this study was to evaluate binding of four targets of biodefense interest to immobilized antimicrobial peptides (AMPs) in biosensor assays. Polymyxins B and E, melittin, cecropins A, B, and P, parasin, bactenecin and magainin-1, as well as control antibodies, were used as capture molecules for detection of Cy3-labeled Venezuelan equine encephalitis virus (VEE), vaccinia virus, C. burnetti and B. melitensis. Although VEE, vaccinia virus and C. burnetti did not show any binding activity to their corresponding capture antibodies, B. melitensis bound to immobilized anti-Brucella monoclonal antibodies. The majority of the immobilized AMPs included in this study bound labeled VEE, vaccinia virus and C. burnetti in a concentration-dependent manner, and B. melitensis bound to polymyxin B, polymyxin E, and bactenecin. No binding was observed on immobilized magainin-1. In contrast to all bacterial targets tested to date, VEE and vaccinia virus demonstrated similar patterns of binding to all peptides. While the direct assay is generally replaced by a sandwich assay for analysis of real-world samples, direct binding experiments are commonly used to characterize specificity and sensitivity of binding molecules. In this case, they clearly demonstrate the capability of AMPs as recognition molecules for four biothreat agents.

US$19.90

PMID: 18231571

DOI: 10.1016/j.snb.2006.09.044


Other references

Isoda, K.; Maeda, T.; Hamamoto, Y., 1987: Collagen-producing mesothelial cells in adriamycin-induced pleuritis in rat. Microautoradiographic study utilizing tritiated proline. By the microautoradiographic method using 3H-proline, collagen production by mesothelial cells was investigated in adriamycin-induced pleuritis in rats. In subpleural granulation tissue formed at 4 to 7 days after the intrapleural injection of adr...

Pardos, M.; Benninghoff, C.; Guéguen, C.; Thomas, R.; Dobrowolski, J.; Dominik, J., 2000: Acute toxicity assessment of Polish (waste) water with a microplate-based Hydra attenuata assay: a comparison with the Microtox test. The use of Hydra attenuata in acute toxicity assessment is a potentially useful tool in (waste) water biomonitoring. The purpose of this study was to compare the sensitivity of H. attenuata with the extensively used Microtox test on 14 (waste) wat...

Rosenblum, I., 1972: Effects of drugs and chemicals on subcellular organelles: mitochondrial contraction and coronary dilators. Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine 141(3): 932-935

Darby, W.J.; Jones, E., 1949: Observations on two newer hemopoietic vitamins, vitamin B12 and animal protein factor. American Journal of Medicine 7(2): 241-241

Seok, H-Yeon.; Park, H-Yeon.; Park, J-Im.; Lee, Y-Mi.; Lee, S-Young.; An, G.; Moon, Y-Hwan., 2010: Rice ternary MADS protein complexes containing class B MADS heterodimer. To investigate ternary MADS protein complexes involved in the regulation of floral organ development in rice, we identified MADS proteins interacting with the class B MADS heterodimers, OsMADS16-OsMADS4 and OsMADS16-OsMADS2, using yeast three-hybr...

Kapelak, B.; Sadowski, J.; Pfitzner, R.; Garlicki, M.; Zmudka, K.; Pietrzak, I.; Podolec, P.; Pieniazek, P.; Drwila, R.; Kozanecki, A.; Wójcik, S.; Pietrzyk, E.; Grudzień, G.; Rudziński, P.; Dziatkowiak, A., 1999: Coronary bypass grafting after failed percutaneous angioplasty (PTCA). Between January 1991 and September 1997, in the Cardiovascular Surgery Department of the Institute of Cardiology of Jagiellonian University Medical School, 23 patients underwent emergency CABG due to acute myocardial ischaemia in result of failed...

Harasewych, M.G.; Kiel, S., 2007: Upper Jurassic Pleurotomariidae (Gastropoda) from southwestern Madagascar. This paper describes four new species of Upper Jurassic Pleurotomariidae from southwestern Madagascar: Obornella thompsonorum, Bathrotoniaria annejoffeae, Eathrotomaria bedetteae, and Leptomaria takahashii. In addition, the previously described Le...

Pearlman, S., 1968: Pearlman named to dental school. Journal of the Colorado Dental Association 46(2): 11-11

Romey, William L., 1996: Individual differences make a difference in the trajectories of simulated schools of fish. A simulation model was designed in which each individual in a group can be programmed with a different set of movement decision rules based on: the distance of others within the group, velocity of individuals, its own previous vector (momentum), a...

Kram Mark; Lory Ernest, 1998: Use of SCAPS suite of tools to rapidly delineate a large MTBE plume. Proceedings of the Symposium on the Application of Geophysics to Environmental and Engineering Problems (SAGEEP): Pages 85-99. 1998.