+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Antioxidant and hepatoprotective effect of (-)-epigallocatechin 3-O-(3-O-methyl) gallate (EGCG3″Me) from Chinese oolong tea

, : Antioxidant and hepatoprotective effect of (-)-epigallocatechin 3-O-(3-O-methyl) gallate (EGCG3″Me) from Chinese oolong tea. Journal of Agricultural and Food Chemistry 62(41): 10046-10054

(-)-Epigallocatechin 3-O-(3-O-methyl) gallate (EGCG3″Me) has exhibited various biological activities in oolong tea. However, little information about its hepatoprotective activity is available. The objectives of the present study, therefore, were to determine the hepatoprotective activity of EGCG3″Me. First, high-purity EGCG3″Me was prepared from Chinese oolong tea by column chromatography. In antioxidant assay in vitro, EGCG3″Me exhibited potential antioxidant activity. For hepatoprotective activity in vitro, it was observed that EGCG3″Me effectively alleviated the changes induced by alcohol in a concentration-dependent manner. For hepatoprotective activity in vivo, the administration of EGCG3″Me at a dose of 100 mg/kg BW per day significantly decreased the serum levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) from 64.6 ± 3.17 and 97.6 ± 3.78 to 39.6 ± 2.72 and 59.6 ± 3.02 U/L, decreased the liver level of malondialdehyde (MDA) from 1.14 ± 0.08 to 0.77 ± 0.03 nmol/mg protein, and remarkably restored the liver activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) from 247 ± 20.1 U/mg and 6.12 ± 0.17 nmol/mg protein to 261 ± 9.98 U/mg and 8.10 ± 0.03 nmol/mg protein, respectively, in alcohol-induced liver injury mice. This suggested that the protective effect of EGCG3″Me against alcohol-induced liver injury is possibly via its antioxidant activity to protect biological systems against oxidative stress.


PMID: 25259858

DOI: 10.1021/jf5016335

Other references

Rozsos, I., 1973: Pathological reflexes of the ileocecal region and the gastroduodenal tract. Orvosi Hetilap 114(50): 3022-3025

Burnett, Lou, 2010: Steve Morris 1956-2009. Comparative Biochemistry and Physiology Part A Molecular & Integrative Physiology 155(1): 12-13

Naito, K.; Skog, S.; Tribukait, B.; Andersson, L.; Hisazumi, H., 1987: Cell cycle related [3H]thymidine uptake and its significance for the incorporation into DNA. Cellular uptake of [3H]thymidine [( 3H]TdR) and incorporation into DNA of Ehrlich ascites tumour cells were studied in relation to the cell cycle by measuring the activity in the acid-soluble and insoluble parts of the cell material. Cells were sy...

Zee D.S.; Yamazaki A.; Butler P.H.; Gucer G., 1981: Effects of ablation of flocculus and paraflocculus on eye movements in primate macaca mulatta. Eye movements were recorded in 4 rhesus monkeys [M. mulatta] before and after bilateral ablations of the flocculi and portions of the paraflocculi (flocculectomy). Animals were trained to fixate and follow targets so that pursuit, saccades and ves...

Job, J.C.; Joab, N.; Toublanc, J.E.; Canlorbe, P., 1984: Final results of treatment with human growth hormone. The authors report the results of treatment with human growth hormone (hGH) (for 3 1/2 years to 11 3/4 years, mean: 5 years 4 months) in 34 children presenting with complete growth hormone deficiency and an average growth failure reaching 3.9 stan...

Jansen, L.; Schulz, S., 2011: Grains, components and mixtures in biomedical ontologies. In biomedical ontologies, mereological relations have always been subject to special interest due to their high relevance in structural descriptions of anatomical entities, cells, and biomolecules. This paper investigates two important subrelation...

Focà, E.; Magro, P.; Motta, D.; Compostella, S.; Casari, S.; Bonito, A.; Brianese, N.; Ferraresi, A.; Rodari, P.; Pezzoli, M.Chiara.; Quiros-Roldan, E.; Castelli, F., 2016: Screening for Neurocognitive Impairment in HIV-Infected Individuals at First Contact after HIV Diagnosis: The Experience of a Large Clinical Center in Northern Italy. Neurocognitive disorders are emerging, probably underestimated, complications in HIV-infected people. The aim of the study was to assess neurocognitive profiles of newly detected HIV-infected patients. We performed an observational retrospective s...

Gong Nengping; L.W.i, 1996: Correction of a theory for testing the tensile strength of rock-like materials by hydraulic fracturing. The classic theory for testing the tensile strength of rock by hydraulic fracturing is to regard the materials as homogeneous, isotropic, linear elastomers. However, rock-like material has the deformation property that the tensile elastic modulus...

Kempson, R.L.; Pokorny, G.E., 1968: Adenocarcinoma of the endometrium in women aged forty and younger. Twenty-two women aged 40 years and younger were diagnosed as endometrial carcinoma at Barnes Hospital from 1930 to 1967. Seven of these were rediagnosed as hyperplasia on review of the histologic sections; the remaining 15 had endometrial changes...

Vincent, P.G.; Kulik, M.M., 1973: Pyrolysis-gas-liquid chromatography of fungi: numerical characterization of species variation among members of the Aspergillus glaucus group. Mycopathologia et Mycologia Applicata 51(4): 251-265