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Array-based capture, distribution, counting and multiplexed assaying of beads on a centrifugal microfluidic platform

, : Array-based capture, distribution, counting and multiplexed assaying of beads on a centrifugal microfluidic platform. Lab on A Chip 12(7): 1289-1295

We present a novel centrifugal microfluidic platform for the highly efficient manipulation and analysis of particles for applications in bead-based assays. The platform uses an array of geometrical V-cup barriers to trap particles using stopped-flow sedimentation under highly reproducible hydrodynamic conditions. The impact parameters governing the occupancy distribution and capture efficiency of the arrayed traps are investigated. The unique, nearly 100% capture efficiency paired with the capability to establish sharply peaked, single occupancy distributions enables a novel, digital readout mode for color-multiplexed, particle-based assays with low-complexity instrumentation. The presented technology marks an essential step towards a versatile platform for the integration of bead- and cell-based biological assays.


PMID: 22334354

DOI: 10.1039/c2lc21170j

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