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Association of gastric emptying with ghrelin, obestatin and receptor (GHSR, GPR-39) in hypothalamus of diabetic rats


, : Association of gastric emptying with ghrelin, obestatin and receptor (GHSR, GPR-39) in hypothalamus of diabetic rats. Zhonghua Yi Xue Za Zhi 90(16): 1137-1140

To investigate the association of gastric emptying with ghrelin, obestatin and GHSR, GPR-39 in hypothalamus of diabetic rats. Sixty Wistar rats were randomly divided into three groups: a normal control group (NC, n = 20), a diabetes mellitus group (DM, n = 20) induced by intraperitoneal injection of streptozotocin (STZ) and an insulin treated group (INS, n = 20). After two and six weeks of STZ injection, gastric emptying was measured by intragastric administration of phenol red, ghrelin and obestatin in hypothalamus measured by ELISA (enzyme-linked immunosorbent assay) and GHSR and GPR-39 by RT-PCR (reverse transcription-polymerase chain reaction). After two weeks of STZ injection, gastric emptying (%) (74 +/- 8, 40 +/- 5), ghrelin level(ng/g) (52 +/- 9, 51 +/- 7) and ratio of ghrelin/obestatin (3.8 +/- 1.0, 2.8 +/- 1.0) increased significantly in DM and INS groups compared to those in NC group [32% +/- 7%, (39 +/- 11) ng/g, 2.1 +/- 0.8, all P < 0.05]. Obestatin level(ng/g) (14.2 +/- 2.0) of hypothesis decreased significantly in DM group as compared to those in NC group (21.7 +/- 4.7) while GHSR/beta-actin increased significantly (1.26 +/- 0.46 vs 0.77 +/- 0.21, P < 0.05). Gastric emptying was positively correlated with ghrelin, ghrelin/obestatin and GHSR/beta-actin of hypothalamus (r = 0.49; r = 0.63; r = 0.73; P < 0.01). But there was a negative correlation with obestatin of hypothalamus (r = -0.74, P < 0.01). After six weeks of STZ injection, gastric emptying (78.97% +/- 8.13% vs 44.06% +/- 5.06%) increased significantly in DM and INS groups as compared to those in NC group (35.06% +/- 3.91%, P < 0.01). Gastric emptying was positively correlated with ghrelin/obestatin of hypothalamus (r = 0.40, P < 0.05). There was no detection of GPR-39 in hypothalamus. The rapid gastric emptying may be due to the rising levels of ghrelin and GHSR in hypothalamus during early hyperglycemia. And the duration of hyperglycemia is affected by the rising ratio of ghrelin/obestatin.

US$29.90

PMID: 20646435


Other references

Melamed, N.B.; Satya-Murti, S., 1983: Obturator neuropathy after total hip replacement. Annals of Neurology 13(5): 578-579

Ferguson R.A., 1987: Innovative environmental management approaches for cost effective control of industrial residuals. Draggan, S. Cohrssen And R. E. Morrison (ed.). Environmental Monitoring Assessment, And Management: The Agenda For Long-Term Research And Development. Xxxv 128p. Praeger Publishers: New York, New York, Usa; London, England, Uk. Illus. 109-124

Bersani, F.Saverio.; Minichino, A.; Fattapposta, F.; Bernabei, L.; Spagnoli, F.; Mannarelli, D.; Francesconi, M.; Pauletti, C.; Corrado, A.; Vergnani, L.; Taddei, I.; Biondi, M.; Delle Chiaie, R., 2015: Prefrontocerebellar transcranial direct current stimulation increases amplitude and decreases latency of P3b component in patients with euthymic bipolar disorder. Neurocognitive impairments have been observed in patients with bipolar disorder (BD) even during the euthymic phase of the disease, potentially representing trait-associated rather than state-associated characteristics of the disorder. In the pres...

Michels, K., 1990: Determination of herbicides in soils. A universal analytical method based on HPLC was developed by which 31 herbicides can be analysed in soils in concentrations of 0.01 mg active ingredient /kg soil. Substances to which this methodology applies include: aryloxycarboxylic acids, benzo...

Cameron, Robert., 1992: Recent reports. Molluscs. A new snail for Shropshire. Shropshire Naturalist. Spring; 11: 45

Harman S.H., 1935: Some tin deposits of the Burma-Malayan peninsula. Mining Magazine (London) 52(3): 148-152

Subramaniam, V.Nathan., 2016: Pocket-sized iron regulators: one size fits all?. Blood 128(2): 153-154

Pellicci, P.M.; Wilson, P.D., 1979: Chondrolysis of the hips associated with severe burns. A case report. A 27 yr old man is presented with bilateral chondrolysis of the hip joint associated with severe burns. Pelvic roentgenograms are employed to evaluate joint erosion prior to prosthetic total hip replacement. Histologic chondrolysis is found follow...

Degtyarev, PA.; Fukshanskij, LYa., 1973: The effect of photic rhythms on the biological clock phase. Zhurnal obshch Biol, 345: 738-744

Maldonado, D.Jr, 1977: The Mexican American grows old. Later years: social applications of gerontology edited by Richard A Kalish: 3