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Bacteriocin protein BacL1 of Enterococcus faecalis targets cell division loci and specifically recognizes L-Ala2-cross-bridged peptidoglycan


, : Bacteriocin protein BacL1 of Enterococcus faecalis targets cell division loci and specifically recognizes L-Ala2-cross-bridged peptidoglycan. Journal of Bacteriology 197(2): 286-295

Bacteriocin 41 (Bac41) is produced from clinical isolates of Enterococcus faecalis and consists of two extracellular proteins, BacL1 and BacA. We previously reported that BacL1 protein (595 amino acids, 64.5 kDa) is a bacteriolytic peptidoglycan D-isoglutamyl-L-lysine endopeptidase that induces cell lysis of E. faecalis when an accessory factor, BacA, is copresent. However, the target of BacL1 remains unknown. In this study, we investigated the targeting specificity of BacL1. Fluorescence microscopy analysis using fluorescent dye-conjugated recombinant protein demonstrated that BacL1 specifically localized at the cell division-associated site, including the equatorial ring, division septum, and nascent cell wall, on the cell surface of target E. faecalis cells. This specific targeting was dependent on the triple repeat of the SH3 domain located in the region from amino acid 329 to 590 of BacL1. Repression of cell growth due to the stationary state of the growth phase or to treatment with bacteriostatic antibiotics rescued bacteria from the bacteriolytic activity of BacL1 and BacA. The static growth state also abolished the binding and targeting of BacL1 to the cell division-associated site. Furthermore, the targeting of BacL1 was detectable among Gram-positive bacteria with an L-Ala-L-Ala-cross-bridging peptidoglycan, including E. faecalis, Streptococcus pyogenes, or Streptococcus pneumoniae, but not among bacteria with alternate peptidoglycan structures, such as Enterococcus faecium, Enterococcus hirae, Staphylococcus aureus, or Listeria monocytogenes. These data suggest that BacL1 specifically targets the L-Ala-L-Ala-cross-bridged peptidoglycan and potentially lyses the E. faecalis cells during cell division.

US$19.90

PMID: 25368300

DOI: 10.1128/JB.02203-14


Other references

Parker, Bruce C., 1993: Introduction Changing focus of algal biotechnology. Journal of Phycology 29(3 SUPPL ): 5

Stern, D.N.; Fieve, R.R.; Neff, N.H.; Costa, E., 1969: The effect of lithium chloride administration on brain and heart norepinephrine turnover rates. Psychopharmacologia 14(4): 315-322

Li, Z.; Yu, Y.; Li, Z.; Wu, T., 2016: A review of biosensing techniques for detection of trace carcinogen contamination in food products. Carcinogen contaminations in the food chain, for example heavy metal ions, pesticides, acrylamide, and mycotoxins, have caused serious health problems. A major objective of food-safety research is the identification and prevention of exposure to t...

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Kumar A.R.mesh; Kumar E.S.mpath, 2001: Occurrence of fluoride in groundwater of Kodavanar Watershed, Dindigul District, Tamilnadu. Fluorine has significant effect on human health as because of its highly reactive nature it easily gets absorbed in the human system. The most commonly noticed effect of excess fluorine in human body is in the form of dental and skeletal fluorosis...

Rasidovic, D.; Kearney, D.; Boyle, K-Marie.; Bund, S.J., 2010: The assessment of rat ureteral pressure generation in vitro: regional heterogeneity and influence of distending pressure. Contraction of ureteral smooth muscle drives the urine bolus to the urinary bladder for storage prior to micturition. This study describes a novel approach to the measurement of ureteral pressure generation in vitro and the influence of distending...

Lindskog, M.; Winman, A.; Juslin, P., 2014: The association between higher education and approximate number system acuity. Humans are equipped with an approximate number system (ANS) supporting non-symbolic numerosity representation. Studies indicate a relationship between ANS-precision (acuity) and math achievement. Whether the ANS is a prerequisite for learning math...

Larsson P.T.; Olsson G.; Hjemdahl P., 1990: Influence of beta 1 blockade on alterations in human platelet aggregability by mental stress and adrenaline infusions. European Journal of Pharmacology 183(2): 332

Dyuzhikov O.A.; Distler V.V.; Kavardin G.I.; Golubkov V.S.; Sluzhenikin S.F., 1986: Geologic position, deep structure and ore-magmatic systems in the Norilsk region. Pages 204-219 1986

Kohyama K.; Matsunaga R., 1987: Calculation of energy intensities. The energy intensity is the total amount of energy required for the unit production (per 1 yen) of a specified industrial sector, which is the sum of the direct energy requirement and that of the indirect one including the fixed capital consumptio...