geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Cadmium and copper inhibit both DNA repair activities of polynucleotide kinase


, : Cadmium and copper inhibit both DNA repair activities of polynucleotide kinase. Dna Repair 9(1): 83-89

Human exposure to heavy metals is of increasing concern due to their well-documented toxicological and carcinogenic effects and rising environmental levels through industrial processes and pollution. It has been widely reported that such metals can be genotoxic by several modes of action including generation of reactive oxygen species and inhibition of DNA repair. However, although it has been observed that certain heavy metals can inhibit single strand break (SSB) rejoining, the effects of these metals on SSB end-processing enzymes has not previously been investigated. Accordingly, we have investigated the potential inhibition of polynucleotide kinase (PNK)-dependent single strand break repair by six metals: cadmium, cobalt, copper, nickel, lead and zinc. It was found that micromolar concentrations of cadmium and copper are able to inhibit the phosphatase and kinase activities of PNK in both human cell extracts and purified recombinant protein, while the other metals had no effect at the concentrations tested. The inhibition of PNK by environmentally and physiologically relevant concentrations of cadmium and copper suggests a novel means by which these toxic heavy metals may exert their carcinogenic and neurotoxic effects.

US$19.90

PMID: 19962355

DOI: 10.1016/j.dnarep.2009.11.004


Other references

Orbach, D.; M.D.well, H.; Rey, A.; Bouvet, N.; Kelsey, A.; Stevens, M.C., 2012: Sparing strategy does not compromise prognosis in pediatric localized synovial sarcoma: experience of the International Society of Pediatric Oncology, Malignant Mesenchymal Tumors (SIOP-MMT) Working Group. The aim of this analysis was to identify if the modified indications of radiotherapy (RT) or radical surgery compromised survival in pediatric synovial sarcoma (SS). Children with non-metastatic SS, prospectively enrolled in three trials, were ana...

Hourdez, S.; Lallier, F.H.; D.C.an, M.C.cile; Green, B.N.; Weber, R.E.; Toulmond, A., 2000: Gas transfer system in Alvinella pompejana Functional properties of intracellular and extracellular hemoglobins. Alvinella pompejana is a tubicolous polychaete that dwells in the hottest part of the hydrothermal vent ecosystem in a highly variable mixture of vent (350degreeC, anoxic, CO2- and sulfide-rich) and deep-sea (2degreeC, mildly hypoxic) waters. This...

Johansen, B.V., 1977: High resolution bright field electron microscopy of biological specimens. Various parameters which affect the information content in bright field electron micrographs of biological specimens are discussed. Special attention is given to the resolution of phase contrast imaging, specimen supports and radiation damage.

Iyer, C.S.; Kamath, V.A., 1980: Determination of niobium in steels. The determination of niobium at levels of 0.01% and below is required in certain specifications for stainless-steel welding electrodes, containing 2-3% molybdenum and 0.01% titanium. A method has been developed, based on initial extraction of niob...

Mahadevan C.; Visvanath S.N., 1952: On the colour of blue quartz. Proceedings of the Indian Science Congress Part 3: 170

Castillo P.; Gonzalez Pais M.A.; Gomez Barcina A., 1989: The genus gracilacus raski 1962 in spain paratylenchinae tylenchida. During a nematological survey on several natural plant communities at the Sierra de Cazorla, were found 3 species of the genus Gracilacus Raski, 1962 [G. goodeyi, G. peratica and G. steineri]. Morphology and measurements are reported and compared...

Rothschild, M.A.; Oratz, M.; Wimer, E.; Schreiber, S.S., 1961: Studies on albumin synthesis: the effects of dextran and cortisone on albumin metabolism in rabbits studied with albumin-I-131. Journal of Clinical Investigation 40: 545-554

Ballard, D.J., 1996: Hips and knees: state of evidence regarding effectiveness of quality improvement interventions in orthopedic surgery. Mayo Clinic Proceedings 71(2): 208-210

Ein, S.H.; Miller, S.; Rutka, J.T., 2006: Appendicitis in the child with a ventriculo-peritoneal shunt: a 30-year review. Each year, about 270 children are treated at our hospital for appendicitis, and there are 200 ventriculo-peritoneal (VP) shunt procedures. The incidence of primary peritonitis after a VP shunt is 8% to 12%. The purpose of this article is to try an...

Wang, J.K.; Li, J.L.; Li, M.L.; Hua, D.; Chen, H.M., 2006: Assay of DNA-binding proteins with a dsDNA-coupled plate. Objectives: This paper fabricated a cost-effective dsDNA-coupled plate (dcPlate) and applied it to measure the abundance and DNA-binding activity of a DNA-binding protein (DBP).Design and methods: The dcPlate was manufactured by covalently immobil...