+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Cartilage intermediate layer protein 2 (CILP-2) is expressed in articular and meniscal cartilage and down-regulated in experimental osteoarthritis

, : Cartilage intermediate layer protein 2 (CILP-2) is expressed in articular and meniscal cartilage and down-regulated in experimental osteoarthritis. Journal of Biological Chemistry 286(43): 37758-37767

Using transcriptome profiling to determine differential gene expression between the permanent mouse articular cartilage and the transient growth plate cartilage, we identified a highly expressed gene, Cilp2, which is expressed differentially by articular chondrocytes. CILP-2 is highly homologous to CILP-1 (cartilage intermediate layer protein 1), which is expressed in the intermediate zone of articular cartilage and has been linked to cartilage degenerative diseases. We demonstrated that Cilp2 has a restricted mRNA distribution at the surface of the mouse articular cartilage during development, becoming localized to the intermediate zone of articular cartilage and meniscal cartilage with maturity. Although the extracellular CILP-2 protein localization is broadly similar to CILP-1, CILP-2 appears to be more localized in the deeper intermediate zone of the articular cartilage extracellular matrix at maturity. CILP-2 was shown to be proteolytically processed, N-glycosylated, and present in human articular cartilage. In surgically induced osteoarthritis in mice, Cilp1 and Cilp2 gene expression was dysregulated. However, whereas Cilp1 expression was increased, Cilp2 gene expression was down-regulated demonstrating a differential response to mechanically induced joint destabilization. CILP-2 protein was reduced in the mouse osteoarthritic cartilage. Ultrastructural analysis also suggested that CILP-2 may be associated with collagen VI microfibrils and thus may mediate interactions between matrix components in the territorial and inter-territorial articular cartilage matrix. mRNA expression analysis indicated that whereas Cilp1 and Cilp2 are expressed most abundantly in cartilaginous tissues, expression can be detected in muscle and heart.


PMID: 21880736

DOI: 10.1074/jbc.M111.248039

Other references

Scoggin, J.F.; Whipple, T.L., 1992: A potential complication of endoscopic carpal tunnel release. Complications of carpal tunnel release have been well documented in the literature. Recently, a procedure for endoscopic release of the transverse carpal ligament has been described. This case report demonstrates a potential complication of endosc...

Hyndman Donald W.; Alt David; Nold John L., 1968: Some limits to diffusion during regional metamorphism north of the Idaho Batholith. Special Paper - Geological Society of America Pages 106

Ferrante, A.; Robinson, B.S.; Singh, H.; Jersmann, H.P.A.; Ferrante, J.V.; Huang, Z.H.; Trout, N.A.; Pitt, M.J.; Rathjen, D.A.; Easton, C.J.; Poulos, A.; Prager, R.H.; Lee, F.S.; Hii, C.S.T., 2006: A novel beta-oxa polyunsaturated fatty acid downregulates the activation of the IkappaB kinase/nuclear factor kappaB pathway, inhibits expression of endothelial cell adhesion molecules, and depresses inflammation. Several novel polyunsaturated fatty acids (PUFAs) that contain either an oxygen or sulfur atom in the beta-position were found to exhibit more selective antiinflammatory properties than their natural PUFA counterparts. One of these, beta-oxa-23:4n...

Fechtner, L.; E.A.i, M.; Sattar, A.; Moore, M.; Strohl, K.P., 2016: Fentanyl effects on breath generation in C57BL/6J and A/J mouse strains. We examined the effect of fentanyl on chemoresponsiveness in mouse strains divergent in the expression of spontaneous and post-hypoxic pauses. Frequency and tidal volume were recorded with plethysmography in A/J and C57BL/6J (B6) male mice. Mice s...

Kumar, P.; Heiss, C.; Santiago-Tirado, F.H.; Black, I.; Azadi, P.; Doering, T.L., 2015: Pbx proteins in Cryptococcus neoformans cell wall remodeling and capsule assembly. The cryptococcal capsule is a critical virulence factor of an important pathogen, but little is known about how it is associated with the cell or released into the environment. Two mutants lacking PBX1 and PBX2 were found to shed reduced amounts o...

Heyn, H.; Esteller, M., 2013: EZH2: an epigenetic gatekeeper promoting lymphomagenesis. In this issue of Cancer Cell, Béguelin and colleagues highlight EZH2 as an essential regulator for B cell activation and report an addiction of germinal center-derived neoplasms to EZH2 activity. This reversible process is specifically targetable...

Mikula I.; Pistl J., 1990: The use of the migration inhibition test of leukocytes lmi in the assessment of the activity of leukocytic dialysate specific for salmonella spp. The capillary migration inhibition test of leucocytes was administered to assay the activity of leukocytic dialysate (DLE). For this purpose there was used DLE derived from animals infected with S. typhimurium (DLEinf.), immunized with a vaccine c...

Wedel, K.W., 1955: Use of pyridoxin in postanesthetic vomiting. Der Anaesthesist 4(4): 122-123

Rossi, G., 1987: A flourishing art: China--Guizhou women continue to embroider their legends. Threads magazine: (9) 30-32

Meienhofer, Johannes, 1966: Synthesis of a decapeptide derivative with the sequence 21 - 30 of the B-chain of human insulin. The C-terminal decapeptide (B 21 - 30) of the human insulin B-chain has been prepared as protected derivative, (benzyl-oxycarbonyl-L-glutamyl-gamma-tert. -butylester-w-tosyl-L-arginyl-glycyl-L-phenyl-alanyl-L-phenylalanyl-L-trosyl-L-threonyl-L-pro...