+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Caspase assays: identifying caspase activity and substrates in vitro and in vivo

, : Caspase assays: identifying caspase activity and substrates in vitro and in vivo. Methods in Enzymology 446: 351-367

The measurement of general caspase activity and the quantification of purified recombinant caspases in vitro can be accomplished with relative ease. But the determination of which caspases are active in a cellular context is much more challenging. This is because commercially available small molecule substrates and inhibitors do not display sufficient specificity to dissect the complex interplay of caspase pathways. Here we describe procedures that can be used to validate which caspases are active in cell culture models and determine which caspases are responsible for specific cleavage events. We also recommend methods for working with recombinant initiator caspases in vitro and suggest ways to accurately assess the cleavage efficiency of natural caspase substrates.


PMID: 18603133

DOI: 10.1016/S0076-6879(08)01621-2

Other references

Chihota, C.M.; Rennie, L.F.; Kitching, R.P.; Mellor, P.S., 2001: Mechanical transmission of lumpy skin disease virus by Aedes aegypti (Diptera: Culicidae). Aedes aegypti female mosquitoes are capable of the mechanical transmission of lumpy skin disease virus (LSDV) from infected to susceptible cattle. Mosquitoes that had fed upon lesions of LSDV-infected cattle were able to transmit virus to suscepti...

Landen; Winfield; Burgess; Kilkenny; Lee, 1985: Production of dense, cool plasmas by resonance pumping of sodium vapor. Physical Review. A, General Physics 32(5): 2963-2971

Pertseva, A.N., 1950: Microbiological processes in the composting of deep-lying peat.. Previous work had shown that deep-lying bog peat had a much richer microflora than surface peat. Microbiological studies were made of the composting of deep-lying peat with lime and P and N fertilizers. During the first 15 days ammonifiers develop...

Woods R., 1992: Water management at Tarong. Pages 66-77 1992

Sahoo, N.Gopal.; Bao, H.; Pan, Y.; Pal, M.; Kakran, M.; Cheng, H.Kuo.Feng.; Li, L.; Tan, L.Poh., 2011: Functionalized carbon nanomaterials as nanocarriers for loading and delivery of a poorly water-soluble anticancer drug: a comparative study. Carbon nanomaterials such as multiwalled carbon nanotubes (MWCNTs) and graphene oxide (GO) have been functionalized by highly hydrophilic and biocompatible poly(vinyl alcohol) (PVA) for loading and delivery of an anticancer drug, camptothecin (CPT...

Asano, T.; Kume, H.; Taki, F.; Ito, S.; Hasegawa, Y., 2011: Thalidomide attenuates airway hyperresponsiveness and eosinophilic inflammation in a murine model of allergic asthma. Asthma is characterized by chronic eosinophilic inflammation and hyperresponsiveness of the airways. We hypothesized that thalidomide, which has numerous immunomodulatory properties, may have anti-inflammatory effects in allergic asthma. BALB/c mi...

Jacober, K.Emmerle, M.P., 1956: Prophylaxis and therapy of antibiotic side-effects. Giornale Italiano di Chemioterapia 3(3-4): 602-608

Gaylor, J.L., 1966: Inhibition of cholesterol biosynthesis. New York State Journal of Medicine 66(9): 1097-1099

Rosenbaum, J., 2002: Intraflagellar transport. Current Biology 12(4): R125-R125

Thomson, N., 2014: Harm reduction history, response, and current trends in Asia. HIV epidemics in Asia have been initially driven through injecting drug use and the use of shared needles and syringes. Molecular epidemiological work has shown that where there is heroin trafficking and use, so too is there HIV. Given the often s...