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Cell activity analysis by capillary zone electrophoresis combined with specific cell staining

, : Cell activity analysis by capillary zone electrophoresis combined with specific cell staining. Electrophoresis 32(3-4): 455-463

Capillary zone electrophoresis (CZE) was used to determine Hela cells activity with Hela treated by 0-46 μM methylmercury (MeHg) as the apoptosis model. The treated and untreated cells were stained by four different dyes (Janus Green B, Rhodamine 123, Neutral Red and Trypan Blue) and analyzed by CZE with UV/Vis detection. The absorbance of cells at 214 nm could indicate the degree of cell shrinkage and component leakage induced by MeHg. The intensity of cell absorbance at maximum visible absorption wavelength of dyes represented mitochondrial activity, lysosome phagocytosis ability and cell membrane integrity. For different concentrations of MeHg treatment, the change of cell activity was in good agreement with Janus Green B uptake colorimetric assay (R² =0.914) and 3-(4, 5-dimethylthiazol-2-yl)-2, 5-di-phenyltetrazolium bromide (MTT) assay (R² =0.892). 80% of RSD (n=3) values were in the range of 0.5-15.0%. The established CZE method could be used to analyze intact cells with only UV-Vis detector. The CZE method has some features equivalent to the existing universal method, and it has the potential to be a universal tool for cell activity determination.


PMID: 21298670

DOI: 10.1002/elps.201000324

Other references

Bowen, T.J., 1966: Modern trends in ultracentrifugation. Laboratory Practice 15(3): 292-298

Vodrazka, J., 1960: Evaluation of diethylcarbamazine and cyanacethydrazide against lungworms in calves by tracheotomy.. Agauzebag for collecting worms expelled from the lungs was connected to the trachea of 33 calves with moderate to severe husk. Diethylcarbamazine at a daily dose of 20 mg./kg. was then given i/m to 18 calves for 3 days; 3, 011 (67.8%) of 4, 438 lu...

Tinwell, H.; Rascle, J.B.; Colombel, S.; A.K.ansa, I.; Freyberger, A.; Bars, R., 2012: A novel method for measuring aromatase activity in tissue samples by determining estradiol concentrations. Increasing scrutiny of endocrine disrupters has led to changes to European pesticide and biocide legislation and to the introduction of the Endocrine Disrupter Screening Program by the US EPA. One element of endocrine disrupter identification is t...

Kirichenko, I.Ia., 1972: Control of injuries in children--an important task of public health agencies. Meditsinskaia Sestra 31(4): 44-47

Guseva N.G., 1990: Local therapy for systemic scleroderma. Terapevticheskii Arkhiv 62(5): 110-113

Karabatak, Mustafa, 1997: The time of spawning and seasonal variations in the length-weight relationship and condition of chub, Leuciscus lepidus in Lake Beysehir. The chub showed a peak in spawning in June. The weight and condition (K) at the same lengths and the length-weight regression (W=aLb) coefficients of the sexes did not differ statistically for the same season, but they were slightly higher in fema...

Heinze A.; Stahl U.; Raasch M L., 1991: Synchronization of ovulation of adult sows studies into dosage of gonavet berlin chemie depending on month of mating. The accepted doses of 25 .mu.g of Gonavet "Berlin-Chemie" were used in two series of experiments to stimulate ovulation in roughly 10,800 ovulation-synchronised adult sows, with fertility performance being subsequently determined. Farrow...

Ouyang, Y.; Ma, J., 2006: Classification of multi-spectral remote sensing data using a local transfer function classifier. This paper introduces a new neural network, called the local transfer function classifier (LTF-C), for classification of multi-spectral remote sensing data. The network structure of LTF-C is similar to that of the radial basis function neural netw...

Kardos, I.; Szent-Györgyi, A., 1975: A talk with Albert Szent-Györgyi. New Hungarian Quarterly 16(57): 136-150

Brougher, J.C., 2014: Further studies with methergine and its effect on the pregnant uterus. Western Journal of Surgery, Obstetrics, and Gynecology 55(7): 371-374