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Characterization of n-Hexane sub-fraction of Bridelia micrantha (Berth) and its antimycobacterium activity

, : Characterization of n-Hexane sub-fraction of Bridelia micrantha (Berth) and its antimycobacterium activity. Bmc Complementary and Alternative Medicine 11(): 28-28

Tuberculosis, caused by Mycobacterium tuberculosis (MTB), is the most notified disease in the world. Development of resistance to first line drugs by MTB is a public health concern. As a result, there is the search for new and novel sources of antimycobacterial drugs for example from medicinal plants. In this study we determined the in vitro antimycobacterial activity of n-Hexane sub-fraction from Bridelia micrantha (Berth) against MTB H37Ra and a clinical isolate resistant to all five first-line antituberculosis drugs. The antimycobacterial activity of the n-Hexane sub-fraction of ethyl acetate fractions from acetone extracts of B. micrantha barks was evaluated using the resazurin microplate assay against two MTB isolates. Bioassay-guided fractionation of the ethyl acetate fraction was performed using 100% n-Hexane and Chloroform/Methanol (99:1) as solvents in order of increasing polarity by column chromatography and Resazurin microtiter plate assay for susceptibility tests. The n-Hexane fraction showed 20% inhibition of MTB H37Ra and almost 35% inhibition of an MTB isolate resistant to all first-line drugs at 10 μg/mL. GC/MS analysis of the fraction resulted in the identification of twenty-four constituents representing 60.5% of the fraction. Some of the 24 compounds detected included Benzene, 1.3-bis (3-phenoxyphenoxy (13.51%), 2-pinen-4-one (10.03%), N(b)-benzyl-14-(carboxymethyl) (6.35%) and the least detected compound was linalool (0.2%). The results show that the n-Hexane fraction of B. micrantha has antimycobacterial activity.


PMID: 21481267

DOI: 10.1186/1472-6882-11-28

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Nichita, G.; Sarandan, H.; Padeanu, I.; Casaleanu, I.; Gogoloiu, C., 1981: Eglantine (Rosa canina) seeds for feeding broiler chickens and lambs. From 2 weeks old groups of 35 meat chickens were given for 42 days a diet without or with 1% ground eglantine (Rosa canina) seeds. For 60 days groups of 30 lambs were given a diet with 58% roughage and 42% mixed feed without or with 10 or 20% grou...

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Hartel, H.; Scholtyssek, S.; Friedrich, W., 1970: Effect of form of feed and method of pelleting on fattening performance of broilers given feeds with different levels of protein. In a trial for 49 days 2880 Nichols broiler chickens in groups of 12 males and 12 females were given feeds supplying 3150 kcal metabolisable energy per kg and 26 or 12% protein. Each feed was given as meal or pressed into pellets of 2, 3 or 4 mm w...

Eiche, A., 1975: Mutations induced in two Drosophila melanogaster populations differing in somatic sensitivity to irradiation. Hereditas 9 (1) 149-150

Mclean, B.; Humphries, J.; Juniper, B.E., 1994: A freeze-fracture study of mesophyll cell membranes in the embryonic Phaseolus leaf, in the dry seed and during the initial stages of germination. Using rapid-freezing and freeze-fracturing techniques, we have examined cellular organisation within mesophyll cells in primary leaves of Phaseolus, in the dry seed and over a period of 48 hr from the onset of germination. The rapidly-frozen, unet...

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Miloshenko, V.V.; Rozental' , A.M., 1971: Morphological characteristics of udders of Russian Brown cows on the Stravropol' Institute's teaching farm. Mean 90-day 1st lactation yields of 6 cows with cup-shape udders, 9 with round-shape udders and 2 with goat's udder-shape udders were respectively 911, 635 and 347 kg. Corresponding values for 300-day 2nd-5th lactations of 34, 27 and 2 cows r...

Fleischmann, J.; Resnick, M.I., 1989: Diagnostic approaches to the renal mass. Seminars in Urology 7(3): 153-157

Zerulla, W.; Erhardt, K.; Dressel, J.; Locquenqhien, K.H. von; Leibold, E.; Barth, T., 1999: DMPP - a new nitrification inhibitor for agriculture and horticulture. The mode of action and chemical structure of DMPP (dimethylpyrazolephosphate) are described.