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Characterization of the single-chain Fv-Fc antibody MBP10 produced in Arabidopsis alg3 mutant seeds


, : Characterization of the single-chain Fv-Fc antibody MBP10 produced in Arabidopsis alg3 mutant seeds. Transgenic Research 20(5): 1033-1042

ER resident glycoproteins, including ectopically expressed recombinant glycoproteins, carry so-called high-mannose type N-glycans, which can be at different stages of processing. The presence of heterogeneous high-mannose type glycans on ER-retained therapeutic proteins is undesirable for specific therapeutic applications. Previously, we described an Arabidopsis alg3-2 glycosylation mutant in which aberrant Man(5)GlcNAc(2) mannose type N-glycans are transferred to proteins. Here we show that the alg3-2 mutation reduces the N-glycan heterogeneity on ER resident glycoproteins in seeds. We compared the properties of a scFv-Fc, with a KDEL ER retention tag (MBP10) that was expressed in seeds of wild type and alg3-2 plants. N-glycans on these antibodies from mutant seeds were predominantly of the intermediate Man(5)GlcNAc(2) compared to Man(8)GlcNAc(2) and Man(7)GlcNAc(2) isoforms on MBP10 from wild-type seeds. The presence of aberrant N-glycans on MBP10 did not seem to affect MBP10 dimerisation nor binding of MBP10 to its antigen. In alg3-2 the fraction of underglycosylated MBP10 protein forms was higher than in wild type. Interestingly, the expression of MBP10 resulted also in underglycosylation of other, endogenous glycoproteins.

US$19.90

PMID: 21188635

DOI: 10.1007/s11248-010-9475-5


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Ohnuki-Tierney, E., 1977: The classification of the habitual illnesses of the sakhalin ainu russian sfsr ussr. A detailed description is presented of afflictions which the northwest coast Sakhalin Ainu [Russian, SFSR, USSR] view to be of minor significance in comparison with other bodily and mental afflictions (in which the Ainu perceive an involvement of...

Tsukamura M.; Toyama H., 1969: Effect of orotic acid and 4 amino 5 imidazolecarboxamide orotate on infection of mice with tubercle bacilli antibody formation rabbit. Kekkaku 44(4): 117-121

Green, Hugh R., 1938: Inexpensive soil sample pulverizer. The construction of a soil sample pulverizer from a clothes wringer and 1/4 h.p. motor at a cost of $10.00 is descr. Over 600 samples, including both top and subsoils, were pulverized in the machine in an average of about 1 min. each.

Bruev, S.N.; Kozanova, N.I., 1971: Grape storage. In 6 months' storage trials a high percentage of marketable grapes was obtained from the vine cvs Puhljakovskij (91.1%) and Muscat of Hamburg (90.7%) when the fruit was cooled at 0 to -2 degrees C. for 20-25 days and stored in boxes covered w...

Yamaguchi, H., 1969: The onset of DNA synthesis after soaking of rice seed as disclosed by the induction of mutations. At various times after soaking. rice seeds were treated with X rays. tritiated thymidine and aminopterin. The resulting pattern of mutations suggests that de novo synthesis of DNA in a cell from which meristematic tissue will be formed takes place...

Dürr, W., 1979: Infection following open cavity injuries (thoracic and abdominal cavity). Hefte Zur Unfallheilkunde 138: 151-156

Ogden, John, 1993: Population increase and nesting patterns of the black noddy Anous minutus in Pisonia forest on Heron Island: Observations in 1978, 1979 and 1992. Estimates of the population size of black noddy Anous minutus on Heron Island were made by counting nests in permanently marked plots in Pisonia grandis forest in 1978, 1979 and 1992. The results, and published data, indicate that the population h...

Kilinc M.; Ozkanca R., 1991: The vegetation of the coastal dune of central black sea region. In this study, the vegetation of coastal dune of Central Black Sea region (from Unye to Alacam) has been researched from the phytosociological point of view and the vegetation according to Braun-Blanquet (1) method has been classified in respect o...

Iwasaki, Y.W.; Murano, K.; Ishizu, H.; Shibuya, A.; Iyoda, Y.; Siomi, M.C.; Siomi, H.; Saito, K., 2016: Piwi Modulates Chromatin Accessibility by Regulating Multiple Factors Including Histone H1 to Repress Transposons. PIWI-interacting RNAs (piRNAs) mediate transcriptional and post-transcriptional silencing of transposable element (TE) in animal gonads. In Drosophila ovaries, Piwi-piRNA complexes (Piwi-piRISCs) repress TE transcription by modifying the chromatin...

Rabinovitch, A., 1979: The Canada health survey. Journal of Clinical Computing 8(5): 202-213